LOCUS       40924_45783        4748 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pURHI, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4748)
  AUTHORS   Lv H, Gong Z.
  TITLE     Cost-effective and repeatable gene targeting in fission yeast using 
            a cloning-based and modular method
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4748)
  AUTHORS   Lv H, Gong Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2012) School of Life Science, Fudan University, 
            Room 618, Science Building, Handan Road 220, Shanghai 200433, China
REFERENCE   3  (bases 1 to 4748)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4748)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-MAR-2012) School of Life Science, Fudan University, Room 618, 
            Science Building, Handan Road 220, Shanghai 200433, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4748
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        235..253
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             298..315
                     /label=6xHis
                     /note="6xHis affinity tag"
     regulatory      325..817
                     /label=Saccharomyces cerevisiae Leu2 terminator
                     /note="Saccharomyces cerevisiae Leu2 terminator"
                     /regulatory_class="terminator"
     regulatory      824..1345
                     /label=Schizosaccharomyces pombe Ura4 promoter
                     /note="Schizosaccharomyces pombe Ura4 promoter"
                     /regulatory_class="promoter"
     CDS             1346..2137
                     /label=S. pombe ura4
                     /note="orotidine 5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     regulatory      2141..2570
                     /label=Schizosaccharomyces pombe Ura4 terminator
                     /note="Schizosaccharomyces pombe Ura4 terminator"
                     /regulatory_class="terminator"
     promoter        complement(2637..2655)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2913..3501)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3675..4532)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4533..4637)
                     /label=AmpR promoter
ORIGIN
        1 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
       61 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
      121 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tggacatatt
      181 gtcgttagaa cgcggctaca attaatacat aaccttatgt atcatacaca tacgatttag
      241 gtgacactat agaacgcggc cgccagctga agcttctcga gccccggatc ctctagacac
      301 catcaccatc accattaaac tagagaaaat ccttgcttaa aaagattctc tttttttatg
      361 atatttgtac ataaacttta taaatgaaat tcataataga aacgacacga aattacaaaa
      421 tggaatatgt tcatagggta gacgaaacta tatacgcaat ctacatacat ttatcaagaa
      481 ggagaaaaag gaggatgtaa aggaatacag gtaagcaaat tgatactaat ggctcaacgt
      541 gataaggaaa aagaattgca ctttaacatt aatattgaca aggaggaggg caccacacaa
      601 aaagttaggt gtaacagaaa atcatgaaac tatgattcct aatttatata ttggaggatt
      661 ttctctaaaa aaaaaaaaat acaacaaata aaaaacactc aatgacctga ccatttgatg
      721 gagtttaagt caataccttc ttgaaccatt tcccataatg gtgaaagttc cctcaagaat
      781 tttactctgt cagaaacggc cttaacgacg tagtcgaaga tctcaaatcc cactggctat
      841 atgtatgcat ttgtgttaaa aaagtttgta tagattattt aatctactca gcattctttc
      901 tctaaatagg aatttgttac ttaatggaga aaaaaatgtt tcgatttacc tagtgtattt
      961 gtttgtatac tcacgtttaa tttcaaacat ccattctatc ttgtgtaatt tttggcatgg
     1021 tgaaaaagat aatcagcctt ataatcttta caaaagtaag aaattctgta aataagcctt
     1081 aatgcccttg ctttaaatta aaatggttct ttttcatgat aatgtttgca ctttgtgaat
     1141 atattttaga tagttctgtg aggtataatt aagatgtttt agagacttat acaattttgt
     1201 ctttataaat tcttaattga ttttaccatc ccagtttaac tatgcttcgt cggcatctct
     1261 gcacatgtcg tgttttctta ccgtattgtc ctaccaagaa cctctttttt gcttggatcg
     1321 aaattaaagg tttaaaagca aagttatgga tgctagagta tttcaaagct attcagctag
     1381 agctgagggg atgaaaaatc ccattgccaa ggaattgttg gctttgatgg aagaaaagca
     1441 aagcaacttg tcagtcgcgg tcgatttgac gaagaaatcc gaaatcttag aattggtaga
     1501 taaaattgga ccctatgtct gtgttatcaa gacacatatt gacgttgtcg aggatttcga
     1561 ccaggatatg gtagaaaaac tggtggcctt aggtaaaaag catcgttttc ttatctttga
     1621 ggatcgcaaa ttcgcagaca ttggaaatac cgtcaagcta caatatgcat ctggtgtgta
     1681 caaaattgct tcttgggctc atatcacaaa ttgccataca gtgccaggcg agggtattat
     1741 acagggcctc aaagaagttg gtttaccttt gggacgtggt ctcttgcttt tggctgaaat
     1801 gtcttccaaa ggctctttgg ctactggttc ctacacagag aaaaccttag aatggtttga
     1861 gaagcatacc gatttttgct ttggctttat agctggtcgt cgatttccta accttcaaag
     1921 cgactacata actatgtccc ctggtatcgg cttggatgtt aaaggagacg ggctgggaca
     1981 gcaatatcgt actcctgaag aagtgattgt aaactgcggt agcgatatca tcattgttgg
     2041 tcgtggagtc tatggagctg gtcgtaatcc tgttgtcgaa gccaagagat atagagaagc
     2101 tggttggaag gcatatcagc aaagactttc tcagcattaa aaaaagacta atgtaaaatt
     2161 tttttggttg gttattgaaa aagtcgatgc cttgtttgcg tttgttttcc taggcgtttt
     2221 atgtcagaag gcatttagaa ttagtataca agtactcttt ggtaaaattt tatgtagcga
     2281 ctaaaatatt aactattata gataaacacc ttgggaataa aaagtaattt gctatagtaa
     2341 tttattaaac atgctcctac aacattacca caatcttttc tcttggattg acattgaata
     2401 agaaaagagt gaattttttt agacttgtaa tgataactat gtacaaagcc aatgaaagat
     2461 gtatgtagat gaatgtaaaa taccatgtag acaaacaaga taaaacttgg ttataaacat
     2521 tggtgttgga acagaataaa ttagatgtca aaaagtttcg tcaatatcac gagctcgaat
     2581 tcatcgatga tatcagatcc actagtggcc tatgcggccg cggatctgcc ggtctcccta
     2641 tagtgagtcg tattaatttc gataagccag gttaacctgc attaatgaat cggccaacgc
     2701 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     2761 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     2821 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     2881 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     2941 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     3001 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     3061 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt
     3121 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     3181 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     3241 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     3301 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     3361 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     3421 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     3481 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     3541 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     3601 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     3661 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     3721 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     3781 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     3841 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     3901 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     3961 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     4021 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     4081 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     4141 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     4201 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     4261 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     4321 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     4381 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     4441 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     4501 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     4561 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     4621 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     4681 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt
     4741 ttcggtga
//
