LOCUS       62056_22390        8185 bp DNA     circular SYN 06-APR-2019
DEFINITION  Transformation vector pUPKV, complete sequence.
ACCESSION   MK424962
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8185)
  AUTHORS   Luo J, Shen P, Chen J.
  TITLE     A modular toolset of phiC31-based fluorescent protein tagging 
            vectors for Drosophila
  JOURNAL   Fly (Austin) (2019) In press
  PUBMED    30885036
REFERENCE   2  (bases 1 to 8185)
  AUTHORS   Luo J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2019) Model Animal Research Center, Nanjing 
            University, 12 Xu-fu Rd Pu-kou Dist, Nanjing, Jiangsu 210061, China
REFERENCE   3  (bases 1 to 8185)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fly 
            (Austin) (2019) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-JAN-2019) Model Animal Research Center, Nanjing University, 12 
            Xu-fu Rd Pu-kou Dist, Nanjing, Jiangsu 210061, China"
FEATURES             Location/Qualifiers
     source          1..8185
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    667..2546
                     /label=vermilion
                     /note="vermilion"
     protein_bind    2553..2586
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    2587..3017
                     /label=gypsy insulator
                     /note="chromatin insulator from Drosophila"
     misc_feature    complement(3027..4407)
                     /label=K10 polyA signal sequence
                     /note="K10 polyA signal sequence"
     misc_feature    complement(4457..4519)
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    complement(4501..4745)
                     /label=Delta2-3 promoter
                     /note="Delta2-3 promoter"
     misc_feature    complement(4757..5103)
                     /label=UAS Gal4 binding sites
                     /note="UAS Gal4 binding sites"
     misc_feature    complement(5140..5201)
                     /label=GAGA sites
                     /note="GAGA sites"
     misc_feature    5240..5670
                     /label=gypsy insulator
                     /note="gypsy insulator"
     protein_bind    5709..5778
                     /label=attB
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     promoter        complement(5997..6015)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6036..6052)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6060..6076)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6084..6114)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6129..6150)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6438..7026)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7200..8057)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(8058..8162)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggccccattt gtgtgcgccg cactttcacc tcaagtgatt gataattccc agcctatctg
      721 gcagtgccca tcgcccagat caccgactgt gcaatcagtc ggaactggag ctctctcgct
      781 ctgttatcgg ttcgctgggg tctcatctcc ggtccgctgg cggagatcag ttcgccagca
      841 tccgccgctc gaggagtcac gatctgatct gagctgtgca ccatgagctg tccctatgca
      901 ggaaacgggt gagcaccagc acgtgctgtc caggaatgcc aatcgatctt cagttctgcg
      961 attcaattca aacccataca gaaacgatca cgatgattcg gcggtgccat taaccacgga
     1021 agtgggcaaa atctatggag agtatctgat gctggacaaa ctgctggatg cccagtgtat
     1081 gctgtccgag gaggacaagc gacccgtgca cgatgagcat ctgttcatca tcacgcacca
     1141 gggtgagtag gtttacaact ttgatgacaa cactcaatgg catttaagta ccttcgccac
     1201 agcctacgag ctttggttca agcagatcat ctttgagttc gactccatac gagacatgtt
     1261 ggatgcagag gtcatcgatg aaaccaagac gctggagatt gtcaagcgac tgaaccgagt
     1321 ggttctgatt ctaaaagtga gtgctttctg aatctcttac caaaatccgt ttataacttc
     1381 ctttgtacag ctcctggtgg accaagtgcc cattctggag accatgaccc cgctagactt
     1441 catggacttc cgcaagtacc tggcacccgc atctggtttt cagtcgctgc agttccgttt
     1501 gatcgagaac aagctgggag ttctgacaga gcagcgggtg agatacaacc agaagtactc
     1561 ggatgtcttt agcgacgagg aggcgcggaa ctcgattcgc aactcggaga aagatccctc
     1621 gctactggag ctagtgcagc gatggctgga gaggacgccc ggactggagg agagtggctt
     1681 caacttctgg gccaagtttc aggagagcgt cgatcgattc ctggaggcgc aggtacagag
     1741 cgccatggag gagcccgtgg agaaggcgaa aaactaccgc ctcatggaca ttgagaagcg
     1801 acgcgaggtg tatcgctcca tctttgatcc ggcagtgcac gatgcactgg tgcgtcgtgg
     1861 ggatcgccgg tttagccatc gtgcccttca gggagccatc atgatcacct tctataggga
     1921 tgaacccagg ttcagccaac cacaccagtt gctcaccctg ctcatggaca tcgactcgtt
     1981 aataaccaag tggagatgta agtattgcat tctttgatac tcttttataa atatatctta
     2041 tgtttaagac tggttttcct aaccaaatac tttctattcc cgccgcagac aatcacgtga
     2101 tcatggtgca acgcatgatt ggatcccaac agttgggcac tggtggctcg tctggatatc
     2161 aatatctgcg ctccactctc aggtgatcat cgcagatgtg attatatcgg ggatcaatga
     2221 actcaaactg ttctcccttt gttttttttt ggtttcagtg atcggtacaa ggtgtttctg
     2281 gatctgttca atctgtccac ttttctgatt ccccgcgagg cgattccacc gctggacgag
     2341 accattcgca agaaactgat caacaaaagt gtctgacaat cggcagggta tccaattcgt
     2401 caatgtttgg ctatgcgttg tttgttctgc ctactgtttt gtcgttttgg tgtaataaaa
     2461 ttacttgttt agtctttgtt atcacatttg atgtgttcct tttctttatg tctgacatat
     2521 aatacatata acataacaaa ataaatgcta gtataacttc gtataatgta tgctatacga
     2581 agttattggc cacgtaataa gtgtgcgttg aatttattcg caaaaacatt gcatattttc
     2641 ggcaaagtaa aattttgttg cataccttat caaaaaataa gtgctgcata ctttttagag
     2701 aaaccaaata attttttatt gcatacccgt ttttaataaa atacattgca taccctcttt
     2761 taataaaaaa tattgcatac tttgacgaaa caaattttcg ttgcataccc aataaaagat
     2821 tattatattg catacccgtt tttaataaaa tacattgcat accctctttt aataaagaat
     2881 attgcatacg ttgacgaaac aaattttcgt tgcataccca ataaaagatt attatattgc
     2941 ataccttttc ttgccatacc atttagccga tcaattgtgc tcggcaacag tatatttgtg
     3001 gtgtgccaac caacaacacg cgtacttcag ccaatccgcc gcaccctcag ctccaaagca
     3061 cgcatatgac ccgtgagcag gcagagaaac gcaggcggat aggagtcact agcgcagttc
     3121 tgtatagcct gcactatcaa gccgaagagc tggtagatat tgatgagcac atagtttctg
     3181 gtggagtgca tccagtcaac gccgaaccag gccggataca ggagctctcg atccgggcga
     3241 acgaccacgc ggacgcaact caaagtagtg ccaaatacga aaatactggc aaaggtgcgg
     3301 aaagtgccct gtgcgatatt cacttccttc ctcaaggcag aaatctcctc gggatcgccc
     3361 accagccggg ctctagcgtc catgagcctt aggagagagc gaatctcatg gagctgcttc
     3421 ctcaccatgt acacattcgc aaacttcaaa ttggccacaa tatcggtaat agttatggtc
     3481 aggttctcgc acaatccggc catgttggtg gtgaacagca gcctggccag caagctcaag
     3541 ggaaacagca cggtgaccac caagttgacc gtaatggagt ataccacgta aagcaggctt
     3601 gaaacgcccg gaggacgcat caggccagtg agctcccaaa cgcgccagtg gtagtacaca
     3661 gcactgtggg tgttcagttt gaaatcctct tgcttctcca ttgtctcggt tacctttggt
     3721 caaatccatg ggttctattg cctatatact cttgcgatta ccagtgattg cgctattagc
     3781 tattagatgg attgttggcc aaacttgtcg cttaagtggc tgggaattgt aaccgtaggc
     3841 ccgagtgtaa tgatccccca taaaaagttt tcgcaatgcc tttatttttt gttgcaaatc
     3901 tctctttatt ctgcggtatt cttcattatt gcggggatgg ggaaagtgtt tatatagaag
     3961 caacttacga ttgaacccaa atgcacctga caagcaaggt caaagggcca gatttttaaa
     4021 tatattattt agtcttagga ctctctattt gcaattaaat tactttgcta cctgagggtt
     4081 aaatcttccc cattgataat aataattcca ctatatgttc aattgggttt caccgcgctt
     4141 agttacatga cgagccctaa tgagccgtcg gtggtctata aactgtgcct tacaaatact
     4201 tgcaactctt ctcgttttga agtcagcaga gttattgcta attgctaatt gctaattgct
     4261 tttaactgat ttcttcgaaa ttggtgctat gtttatggcg ctattaacaa gtatgaatgt
     4321 caggtttaac caggggatgc ttaattgtgt tctcaacttc aaaggcagaa atgtttactc
     4381 ttgaccatgg gtttaggtat aatgttatca agctcctcga gttaacgtta cgttaacgtt
     4441 aacgttcgag gtcgactcta gaggatccag atccactagt ggcctatgcg gccgcggatc
     4501 tgatccccgg gcgggtacca atgaacagga cctaacgcac agtcacgtta ttgtttacat
     4561 aaatgatttt ttttactatt caaacttact ctgtttgtgt actcccactg gtatagcctt
     4621 cttttatctt ttctggttca ggctctatca ccggttttca aaaaaaaatt cgtccgcaca
     4681 caacctttcc tctcaacaag caaacgtgca ctgaatttaa gtgtatactt cggtaagctt
     4741 cggctatcga ctcgaccctt gccggaggac agtactccgc cagcccggag gacagtactc
     4801 cgccttgccg gaggacagta ctccgccagc ccggaggaca gtactccgcc ttgccggagg
     4861 acagtactcc gccagcccgg aggacagtac tccgccttgc cggaggacag tactccgcca
     4921 gcccggagga cagtactccg ccttgccgga ggacagtact ccgccagccc ggaggacagt
     4981 actccgcctt gccggaggac agtactccgc cagcccggag gacagtactc cgccttgccg
     5041 gaggacagta ctccgccagc ccggaggaca gtactccgcc ttggaggcct aagcttgatg
     5101 acctcgagaa agagagagag aagagaagag agagaacatt cgagaaagag agagagaaga
     5161 gaagagagag aacattcgag ggggggctag agcggccaat tagatcctgc aggcatgcaa
     5221 gcttggatcc tcaaggcctt ggccacgtaa taagtgtgcg ttgaatttat tcgcaaaaac
     5281 attgcatatt ttcggcaaag taaaattttg ttgcatacct tatcaaaaaa taagtgctgc
     5341 atacttttta gagaaaccaa ataatttttt attgcatacc cgtttttaat aaaatacatt
     5401 gcataccctc ttttaataaa aaatattgca tactttgacg aaacaaattt tcgttgcata
     5461 cccaataaaa gattattata ttgcataccc gtttttaata aaatacattg cataccctct
     5521 tttaataaag aatattgcat acgttgacga aacaaatttt cgttgcatac ccaataaaag
     5581 attattatat tgcatacctt ttcttgccat accatttagc cgatcaattg tgctcggcaa
     5641 cagtatattt gtggtgtgcc aaccaacaac actagagatc cactagtgtc gacgatgtag
     5701 gtcacggtct cgaagccgcg gtgcgggtgc cagggcgtgc ccttgggctc cccgggcgcg
     5761 tactccacct cacccatctg gtccatcatg atgaacgggt cgaggtggcg gtagttgatc
     5821 ccggcgaacg cgcggcgcac cgggaagccc tcgccctcga aaccgctggg cgcggtggtc
     5881 acggtgagca cgggacgtgc gacggcgtcg gcgggtgcgg atacgcgggg cagcgtcagc
     5941 gggttctcga cggtcacggc gggcatgtcg acactagttc tagccagctt ttgttccctt
     6001 tagtgagggt taatttcgag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     6061 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     6121 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     6181 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     6241 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     6301 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     6361 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     6421 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     6481 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     6541 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     6601 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     6661 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     6721 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     6781 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     6841 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     6901 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     6961 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     7021 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     7081 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     7141 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     7201 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     7261 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     7321 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     7381 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     7441 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     7501 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     7561 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     7621 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     7681 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     7741 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     7801 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     7861 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     7921 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     7981 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     8041 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     8101 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     8161 cgcacatttc cccgaaaagt gccac
//
