LOCUS       40924_45773        3378 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUNI(Amp)-GST, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Liu Q, Li MZ, Leibham D, Cortez D, Elledge SJ.
  TITLE     Univector plasmid-fusion system
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Liu Q, Li MZ, Leibham D, Cortez D, Elledge SJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAY-1999) Biochemistry, Baylor College of Medicine, 
            One Baylor Plaza, Houston, TX 77030, USA
REFERENCE   3  (bases 1 to 3378)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3378)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-MAY-1999) Biochemistry, Baylor College of Medicine, One Baylor 
            Plaza, Houston, TX 77030, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3378
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(127..160)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    197..203
                     /label=GST epitope tag cassette
                     /note="GST epitope tag cassette"
     CDS             217..870
                     /codon_start=1
                     /label=GST
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     misc_feature    878..985
                     /label=polylinker region cassette
                     /note="polylinker region cassette"
     polyA_signal    1005..1212
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     terminator      1284..1331
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      complement(1427..1815)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     rep_origin      complement(1830..2285)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(2322..3179)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3180..3284)
                     /label=AmpR promoter
ORIGIN
        1 gctagctgtc cgaaatatta taaattatcg cacacataaa aaccatgctg ttggtgtgtc
       61 tattaaatcg attttttgtt ataacagaca ctgcttgtcc gatatttgat ttaggataca
      121 ggtaccataa cttcgtatag catacattat acgaagttat ctggaattcc ccgggctcga
      181 gaacatatgg ccatggggat ccccgggtta attaatatgt cccctatact aggttattgg
      241 aaaattaagg gccttgtgca acccactcga cttcttttgg aatatcttga agaaaaatat
      301 gaagagcatt tgtatgagcg cgatgaaggt gataaatggc gaaacaaaaa gtttgaattg
      361 ggtttggagt ttcccaatct tccttattat attgatggtg atgttaaatt aacacagtct
      421 atggccatca tacgttatat agctgacaag cacaacatgt tgggtggttg tccaaaagag
      481 cgtgcagaga tttcaatgct tgaaggagcg gttttggata ttagatacgg tgtttcgaga
      541 attgcatata gtaaagactt tgaaactctc aaagttgatt ttcttagcaa gctacctgaa
      601 atgctgaaaa tgttcgaaga tcgtttatgt cataaaacat atttaaatgg tgatcatgta
      661 acccatcctg acttcatgtt gtatgacgct cttgatgttg ttttatacat ggacccaatg
      721 tgcctggatg cgttcccaaa attagtttgt tttaaaaaac gtattgaagc tatcccacaa
      781 attgataagt acttgaaatc cagcaagtat atagcatggc ctttgcaggg ctggcaagcc
      841 acgtttggtg gtggcgacca tcctccaaaa tcggatctgg ttccgcgttg aggcgcgcca
      901 cttctaaata agcggccgcc gagatcatat cactgtggac gttgatgaaa gaatacgtta
      961 ttctttcatc aaatcgtggt cgacgagctc gctatcagcc tcgactgtgc cttctagttg
     1021 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     1081 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     1141 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     1201 gcatgctggg gattctagaa gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
     1261 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
     1321 ggggtttttt gctgaaagga ggaactatat ccggatatca tgcatcgcga gagagagaga
     1381 gagagagaga gagagagaga gagagagaga gacgtgggcc caattctgtc agccgttaag
     1441 tgttcctgtg tcactgaaaa ttgctttgag aggctctaag ggcttctcag tgcgttacat
     1501 ccctggcttg ttgtccacaa ccgttaaacc ttaaaagctt taaaagcctt atatattctt
     1561 ttttttctta taaaacttaa aaccttagag gctatttaag ttgctgattt atattaattt
     1621 tattgttcaa acatgagagc ttagtacgtg aaacatgaga gcttagtacg ttagccatga
     1681 gagcttagta cgttagccat gagggtttag ttcgttaaac atgagagctt agtacgttaa
     1741 acatgagagc ttagtacgtg aaacatgaga gcttagtacg tactatcaac aggttgaact
     1801 gctgatcaac agatcctcta cactagtcta aattgtaagc gttaatattt tgttaaaatt
     1861 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     1921 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     1981 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     2041 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     2101 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     2161 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     2221 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     2281 cgcgtcccat tcgccattca ggctgcacgc gtttcgaatt accaatgctt aatcagtgag
     2341 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     2401 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     2461 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     2521 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     2581 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     2641 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     2701 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     2761 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     2821 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     2881 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     2941 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     3001 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     3061 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     3121 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     3181 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     3241 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     3301 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     3361 atcacgaggc cctttcgt
//
