LOCUS       Exported               11718 bp DNA     circular SYN 10-JUL-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11718)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11718
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      2..590
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    776..916
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      1260..1454
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(1520..2593)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3022..3651)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    4952..4976
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     5179..5195
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5211..7203
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             complement(7241..7546)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFRVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     promoter        complement(7898..7928)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     terminator      7971..8223
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(8240..8256)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8264..8280
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8288..8318)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8333..8354
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8545..9221
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             9284..10081
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    10138..10312
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    10390..10414
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             10839..11633
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
       61 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
      121 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
      181 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
      241 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
      301 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
      361 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
      421 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
      481 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
      541 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
      601 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
      661 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
      721 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
      781 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
      841 gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga
      901 ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
      961 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     1021 gaggttttca ccgtcatcac cgaaacgcgc gaggcagggt gccttgatgt gggcgccggc
     1081 ggtcgagtgg cgacggcgcg gcttgtccgc gccctggtag attgcctggc cgtaggccag
     1141 ccatttttga gcggccagcg gccgcgatag gccgacgcga agcggcgggg cgtagggagc
     1201 gcagcgaccg aagggtaggc gctttttgca gctcttcggc tgtgcgctgg ccagacagtt
     1261 atgcacaggc caggcgggtt ttaagagttt taataagttt taaagagttt taggcggaaa
     1321 aatcgccttt tttctctttt atatcagtca cttacatgtg tgaccggttc ccaatgtacg
     1381 gctttgggtt cccaatgtac gggttccggt tcccaatgta cggctttggg ttcccaatgt
     1441 acgtgctatc cacaggaaag agaccttttc gacctttttc ccctgctagg gcaatttgcc
     1501 ctagcatctg ctccgtacat taggaaccgg cggatgcttc gccctcgatc aggttgcggt
     1561 agcgcatgac taggatcggg ccagcctgcc ccgcctcctc cttcaaatcg tactccggca
     1621 ggtcatttga cccgatcagc ttgcgcacgg tgaaacagaa cttcttgaac tctccggcgc
     1681 tgccactgcg ttcgtagatc gtcttgaaca accatctggc ttctgccttg cctgcggcgc
     1741 ggcgtgccag gcggtagaga aaacggccga tgccgggatc gatcaaaaag taatcggggt
     1801 gaaccgtcag cacgtccggg ttcttgcctt ctgtgatctc gcggtacatc caatcagcta
     1861 gctcgatctc gatgtactcc ggccgcccgg tttcgctctt tacgatcttg tagcggctaa
     1921 tcaaggcttc accctcggat accgtcacca ggcggccgtt cttggccttc ttcgtacgct
     1981 gcatggcaac gtgcgtggtg tttaaccgaa tgcaggtttc taccaggtcg tctttctgct
     2041 ttccgccatc ggctcgccgg cagaacttga gtacgtccgc aacgtgtgga cggaacacgc
     2101 ggccgggctt gtctcccttc ccttcccggt atcggttcat ggattcggtt agatgggaaa
     2161 ccgccatcag taccaggtcg taatcccaca cactggccat gccggccggc cctgcggaaa
     2221 cctctacgtg cccgtctgga agctcgtagc ggatcacctc gccagctcgt cggtcacgct
     2281 tcgacagacg gaaaacggcc acgtccatga tgctgcgact atcgcgggtg cccacgtcat
     2341 agagcatcgg aacgaaaaaa tctggttgct cgtcgccctt gggcggcttc ctaatcgacg
     2401 gcgcaccggc tgccggcggt tgccgggatt ctttgcggat tcgatcagcg gccgcttgcc
     2461 acgattcacc ggggcgtgct tctgcctcga tgcgttgccg ctgggcggcc tgcgcggcct
     2521 tcaacttctc caccaggtca tcacccagcg ccgcgccgat ttgtaccggg ccggatggtt
     2581 tgcgaccgct cacgccgatt cctcgggctt gggggttcca gtgccattgc agggccggca
     2641 ggcaacccag ccgcttacgc ctggccaacc gcccgttcct ccacacatgg ggcattccac
     2701 ggcgtcggtg cctggttgtt cttgattttc catgccgcct cctttagccg ctaaaattca
     2761 tctactcatt tattcatttg ctcatttact ctggtagctg cgcgatgtat tcagatagca
     2821 gctcggtaat ggtcttgcct tggcgtaccg cgtacatctt cagcttggtg tgatcctccg
     2881 ccggcaactg aaagttgacc cgcttcatgg ctggcgtgtc tgccaggctg gccaacgttg
     2941 cagccttgct gctgcgtgcg ctcggacggc cggcacttag cgtgtttgtg cttttgctca
     3001 ttttctcttt acctcattaa ctcaaatgag ttttgattta atttcagcgg ccagcgcctg
     3061 gacctcgcgg gcagcgtcgc cctcgggttc tgattcaaga acggttgtgc cggcggcggc
     3121 agtgcctggg tagctcacgc gctgcgtgat acgggactca agaatgggca gctcgtaccc
     3181 ggccagcgcc tcggcaacct caccgccgat gcgcgtgcct ttgatcgccc gcgacacgac
     3241 aaaggccgct tgtagccttc catccgtgac ctcaatgcgc tgcttaacca gctccaccag
     3301 gtcggcggtg gcccatatgt cgtaagggct tggctgcacc ggaatcagca cgaagtcggc
     3361 tgccttgatc gcggacacag ccaagtccgc cgcctggggc gctccgtcga tcactacgaa
     3421 gtcgcgccgg ccgatggcct tcacgtcgcg gtcaatcgtc gggcggtcga tgccgacaac
     3481 ggttagcggt tgatcttccc gcacggccgc ccaatcgcgg gcactgccct ggggatcgga
     3541 atcgactaac agaacatcgg ccccggcgag ttgcagggcg cgggctagat gggttgcgat
     3601 ggtcgtcttg cctgacccgc ctttctggtt aagtacagcg ataaccttca tgcgttcccc
     3661 ttgcgtattt gtttatttac tcatcgcatc atatacgcag cgaccgcatg acgcaagctg
     3721 ttttactcaa atacacatca cctttttaga cggcggcgct cggtttcttc agcggccaag
     3781 ctggccggcc aggccgccag cttggcatca gacaaaccgg ccaggatttc atgcagccgc
     3841 acggttgaga cgtgcgcggg cggctcgaac acgtacccgg ccgcgatcat ctccgcctcg
     3901 atctcttcgg taatgaaaaa cggttcgtcc tggccgtcct ggtgcggttt catgcttgtt
     3961 cctcttggcg ttcattctcg gcggccgcca gggcgtcggc ctcggtcaat gcgtcctcac
     4021 ggaaggcacc gcgccgcctg gcctcggtgg gcgtcacttc ctcgctgcgc tcaagtgcgc
     4081 ggtacagggt cgagcgatgc acgccaagca gtgcagccgc ctctttcacg gtgcggcctt
     4141 cctggtcgat cagctcgcgg gcgtgcgcga tctgtgccgg ggtgagggta gggcgggggc
     4201 caaacttcac gcctcgggcc ttggcggcct cgcgcccgct ccgggtgcgg tcgatgatta
     4261 gggaacgctc gaactcggca atgccggcga acacggtcaa caccatgcgg ccggccggcg
     4321 tggtggtgtc ggcccacggc tctgccaggc tacgcaggcc cgcgccggcc tcctggatgc
     4381 gctcggcaat gtccagtagg tcgcgggtgc tgcgggccag gcggtctagc ctggtcactg
     4441 tcacaacgtc gccagggcgt aggtggtcaa gcatcctggc cagctccggg cggtcgcgcc
     4501 tggtgccggt gatcttctcg gaaaacagct tggtgcagcc ggccgcgtgc agttcggccc
     4561 gttggttggt caagtcctgg tcgtcggtgc tgacgcgggc atagcccagc aggccagcgg
     4621 cggcgctctt gttcatggcg taatgtctcc ggttctagtc gcaagtattc tactttatgc
     4681 gactaaaaca cgcgacaaga aaacgccagg aaaagggcag ggcggcagcc tgtcgcgtaa
     4741 cttaggactt gtgcgacatg tcgttttcag aagacggctg cactgaacgt cagaagccga
     4801 ctgcactata gcagcggagg ggttggatca aagtactttg atcccgaggg gaaccctgtg
     4861 gttggcatgc acatacaaat ggacgaacgg ataaaccttt tcacgccctt ttaaatatcc
     4921 gattattcta ataaacgctc ttttctctta ggtttacccg ccaatatatc ctgtcaaaca
     4981 ctgatagttt aaactgaagg cgggaaacga caatctgatc caagctcaag ctgctctagc
     5041 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     5101 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     5161 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcagtgca
     5221 gcgtgacccg gtcgtgcccc tctctagaga taatgagcat tgcatgtcta agttataaaa
     5281 aattaccaca tatttttttt gtcacacttg tttgaagtgc agtttatcta tctttataca
     5341 tatatttaaa ctttactcta cgaataatat aatctatagt actacaataa tatcagtgtt
     5401 ttagagaatc atataaatga acagttagac atggtctaaa ggacaattga gtattttgac
     5461 aacaggactc tacagtttta tctttttagt gtgcatgtgt tctccttttt ttttgcaaat
     5521 agcttcacct atataatact tcatccattt tattagtaca tccatttagg gtttagggtt
     5581 aatggttttt atagactaat ttttttagta catctatttt attctatttt agcctctaaa
     5641 ttaagaaaac taaaactcta ttttagtttt tttatttaat aatttagata taaaatagaa
     5701 taaaataaag tgactaaaaa ttaaacaaat accctttaag aaattaaaaa aactaaggaa
     5761 acatttttct tgtttcgagt agataatgcc agcctgttaa acgccgtcga cgagtctaac
     5821 ggacaccaac cagcgaacca gcagcgtcgc gtcgggccaa gcgaagcaga cggcacggca
     5881 tctctgtcgc tgcctctgga cccctctcga gagttccgct ccaccgttgg acttgctccg
     5941 ctgtcggcat ccagaaattg cgtggcggag cggcagacgt gagccggcac ggcaggcggc
     6001 ctcctcctcc tctcacggca ccggcagcta cgggggattc ctttcccacc gctccttcgc
     6061 tttcccttcc tcgcccgccg taataaatag acaccccctc cacaccctct ttccccaacc
     6121 tcgtgttgtt cggagcgcac acacacacaa ccagatctcc cccaaatcca cccgtcggca
     6181 cctccgcttc aaggtacgcc gctcgtcctc cccccccccc cctctctacc ttctctagat
     6241 cggcgttccg gtccatggtt agggcccggt agttctactt ctgttcatgt ttgtgttaga
     6301 tccgtgtttg tgttagatcc gtgctgctag cgttcgtaca cggatgcgac ctgtacgtca
     6361 gacacgttct gattgctaac ttgccagtgt ttctctttgg ggaatcctgg gatggctcta
     6421 gccgttccgc agacgggatc gatttcatga ttttttttgt ttcgttgcat agggtttggt
     6481 ttgccctttt cctttatttc aatatatgcc gtgcacttgt ttgtcgggtc atcttttcat
     6541 gctttttttt gtcttggttg tgatgatgtg gtctggttgg gcggtcgttc tagatcggag
     6601 tagaattctg tttcaaacta cctggtggat ttattaattt tggatctgta tgtgtgtgcc
     6661 atacatattc atagttacga attgaagatg atggatggaa atatcgatct aggataggta
     6721 tacatgttga tgcgggtttt actgatgcat atacagagat gctttttgtt cgcttggttg
     6781 tgatgatgtg gtgtggttgg gcggtcgttc attcgttcta gatcggagta gaatactgtt
     6841 tcaaactacc tggtgtattt attaattttg gaactgtatg tgtgtgtcat acatcttcat
     6901 agttacgagt ttaagatgga tggaaatatc gatctaggat aggtatacat gttgatgtgg
     6961 gttttactga tgcatataca tgatggcata tgcagcatct attcatatgc tctaaccttg
     7021 agtacctatc tattataata aacaagtatg ttttataatt attttgatct tgatatactt
     7081 ggatgatggc atatgcagca gctatatgtg gattttttta gccctgcctt catacgctat
     7141 ttatttgctt ggtactgttt cttttgtcga tgctcaccct gttgtttggt gttacttctg
     7201 caggtcgact ctagaggatc cccgggtacc cgcctgacat ttatattccc cagaacatca
     7261 ggttaatggc gtttttgatg tcattttcgc ggtggctgag atcagccact tcttccccga
     7321 taacggagac cggcacactg gccatatcgg tggtcatcat gcgccagctt tcatccccga
     7381 tatgcaccac cgggtaaagt tcacgggaga ctttatctga cagcagacgt gcactggcca
     7441 gggggatcac catccgtcgc ccgggcgtgt caataatatc actctgtaca tccacaaaca
     7501 gacgataacg gctctctctt ttataggtgt aaaccctaaa ctgcatttca ccagcccctg
     7561 ttctcgtcag caaaagagcc gttcatttca ataaaccggg cgacctcagc catcccttcc
     7621 tgattttccg ctttccagcg ttcggcacgc agacgacggg cttcattctg catggttgtg
     7681 cttaccagac cggagatatt gacatcatat atgccttgag caactgatag ctgtcgctgt
     7741 caactgtcac tgtaatacgc tgcttcatag catacctctt tttgacatac ttcgggtata
     7801 catatcagta tatattctta taccgcaaaa atcagcgcgc aaatacgcat actgttatct
     7861 ggcttttagt aagccggatc ctaactcaaa atccacacat tatacgagcc ggaagcataa
     7921 agtgtaaagc ctggggtgcc taatggagac tagtgagctc gaatttcccc gatcgttcaa
     7981 acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca
     8041 tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat
     8101 ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa
     8161 acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag
     8221 atcgggaatt cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     8281 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     8341 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     8401 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attggctaga
     8461 gcagcttgcc aacatggtgg agcacgacac tctcgtctac tccaagaata tcaaagatac
     8521 agtctcagaa gaccaaaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct
     8581 cctcggattc cattgcccag ctatctgtca cttcatcaaa aggacagtag aaaaggaagg
     8641 tggcacctac aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcctctgc
     8701 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt
     8761 tccaaccacg tcttcaaagc aagtggattg atgtgaacat ggtggagcac gacactctcg
     8821 tctactccaa gaatatcaaa gatacagtct cagaagacca aagggctatt gagacttttc
     8881 aacaaagggt aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca
     8941 tcaaaaggac agtagaaaag gaaggtggca cctacaaatg ccatcattgc gataaaggaa
     9001 aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga
     9061 ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg
     9121 atatctccac tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct
     9181 ctatataagg aagttcattt catttggaga ggacacgctg aaatcaccag tctctctcta
     9241 caaatctatc tctctcgagc tttcgcagat ctgtcgatcg accatgggga ttgaacaaga
     9301 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     9361 acaacagaca atcggctgct ctgatgccgc cgtgtttcgg ctgtcagcgc aggggcgccc
     9421 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactccagg acgaggcagc
     9481 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     9541 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     9601 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     9661 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     9721 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     9781 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
     9841 cgtgacacat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     9901 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     9961 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
    10021 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
    10081 agcgggactc tggggttcgg atcgatcctc tagctagagt cgatcgacaa gctcgagttt
    10141 ctccataata atgtgtgagt agttcccaga taagggaatt agggttccta tagggtttcg
    10201 ctcatgtgtt gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta
    10261 tcaataaaat ttctaattcc taaaaccaaa atccagtact aaaatccaga tcccccgaat
    10321 taattcggcg ttaattcagt acattaaaaa cgtccgcaat gtgttattaa gttgtctaag
    10381 cgtcaatttg tttacaccac aatatatcct gccaccagcc agccaacagc tccccgaccg
    10441 gcagctcggc acaaaatcac cactcgatac aggcagccca tcagtccggg acggcgtcag
    10501 cgggagagcc gttgtaaggc ggcagacttt gctcatgtta ccgatgctat tcggaagaac
    10561 ggcaactaag ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc atgttgattg
    10621 taacgatgac agagcgttgc tgcctgtgat caccgcggtt tcaaaatcgg ctccgtcgat
    10681 actatgttat acgccaactt tgaaaacaac tttgaaaaag ctgttttctg gtatttaagg
    10741 ttttagaatg caaggaacag tgaattggag ttcgtcttgt tataattagc ttcttggggt
    10801 atctttaaat actgtagaaa agaggaagga aataataaat ggctaaaatg agaatatcac
    10861 cggaattgaa aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc
    10921 ctgctaaggt atataagctg gtgggagaaa atgaaaacct atatttaaaa atgacggaca
    10981 gccggtataa agggaccacc tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg
    11041 aaggaaagct gcctgttcca aaggtcctgc actttgaacg gcatgatggc tggagcaatc
    11101 tgctcatgag tgaggccgat ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc
    11161 ctgaaaagat tatcgagctg tatgcggagt gcatcaggct ctttcactcc atcgacatat
    11221 cggattgtcc ctatacgaat agcttagaca gccgcttagc cgaattggat tacttactga
    11281 ataacgatct ggccgatgtg gattgcgaaa actgggaaga agacactcca tttaaagatc
    11341 cgcgcgagct gtatgatttt ttaaagacgg aaaagcccga agaggaactt gtcttttccc
    11401 acggcgacct gggagacagc aacatctttg tgaaagatgg caaagtaagt ggctttattg
    11461 atcttgggag aagcggcagg gcggacaagt ggtatgacat tgccttctgc gtccggtcga
    11521 tcagggagga tatcggggaa gaacagtatg tcgagctatt ttttgactta ctggggatca
    11581 agcctgattg ggagaaaata aaatattata ttttactgga tgaattgttt tagtacctag
    11641 aatgcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    11701 aaaagatcaa aggatctt
//
