LOCUS       40924_45723       10443 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUMat-attB-GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10443)
  AUTHORS   Irion U.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2018) ECNV, Max Planck Institute for Developmental
            Biology, Max-Planck-Ring 5, Tuebingen 72076, Germany
REFERENCE   2  (bases 1 to 10443)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 10443)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (16-MAY-2018) ECNV, Max Planck Institute for Developmental Biology, 
            Max-Planck-Ring 5, Tuebingen 72076, Germany"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10443
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            666..4802
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."
     regulatory      4808..6803
                     /gene="alpha Tub67C"
                     /label=promoter from Drosophila melanogaster
                     /note="promoter from Drosophila melanogaster"
                     /regulatory_class="promoter"
     gene            4808..6803
                     /gene="alpha Tub67C"
                     /label=alpha Tub67C
     CDS             6819..7640
                     /codon_start=1
                     /product="mGFP6"
                     /label=mGFP6
                     /protein_id="AYK27211.1"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALDPNEKRDHMVLLEFV
                     TAAGITHGMDELYKLEVDGIDKLDIEFLQPGGSHMRSRPTSSRACSPGDPI"
     primer_bind     7529..7545
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      7690..7934
                     /label=alphaTub84B 3'UTR
                     /note="alphaTub84B 3'UTR"
                     /regulatory_class="terminator"
     protein_bind    7967..8036
                     /label=attB
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     promoter        complement(8255..8273)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8294..8310)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8318..8334
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8342..8372)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8387..8408)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8696..9284)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9458..10315)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(10316..10420)
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccag gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggcactaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcatt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agcattacat tacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgctattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatttggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagtta gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaaaat aaataaattt catttaactc gcgaacatgt tgaagatatg
     3181 aatattaatg agatgcgagt aacattttaa tttgcagatg gttgccatct tgattggcct
     3241 catctttttg ggccaacaac tcacgcaagt gggcgtgatg aatatcaacg gagccatctt
     3301 cctcttcctg accaacatga cctttcaaaa cgtctttgcc acgataaatg taagtcttgt
     3361 ttagaataca tttgcatatt aataatttac taactttcta atgaatcgat tcgatttagg
     3421 tgttcacctc agagctgcca gtttttatga gggaggcccg aagtcgactt tatcgctgtg
     3481 acacatactt tctgggcaaa acgattgccg aattaccgct ttttctcaca gtgccactgg
     3541 tcttcacggc gattgcctat ccgatgatcg gactgcgggc cggagtgctg cacttcttca
     3601 actgcctggc gctggtcact ctggtggcca atgtgtcaac gtccttcgga tatctaatat
     3661 cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt atcataccat
     3721 tcctgctctt tggcggcttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt
     3781 tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg
     3841 acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca
     3901 aggtcatcct ggagacgctt aacttctccg ccgccgatct gccgctggac tacgtgggtc
     3961 tggccattct catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc
     4021 gacgcaagga gtagccgaca tatatccgaa ataactgctt gttttttttt tttaccatta
     4081 ttaccatcgt gtttactgtt tattgccccc tcaaaaagct aatgtaatta tatttgtgcc
     4141 aataaaaaca agatatgacc tatagaatac aagtatttcc ccttcgaaca tccccacaag
     4201 tagactttgg atttgtcttc taaccaaaag acttacacac ctgcatacct tacatcaaaa
     4261 actcgtttat cgctacataa aacaccggga tatatttttt atatacatac ttttcaaatc
     4321 gcgcgccctc ttcataattc acctccacca caccacgttt cgtagttgct ctttcgctgt
     4381 ctcccacccg ctctccgcaa cacattcacc ttttgttcga cgaccttgga gcgactgtcg
     4441 ttagttccgc gcgattcggt tcgctcaaat ggttccgagt ggttcatttc gtctcaatag
     4501 aaattagtaa taaatatttg tatgtacaat ttatttgctc caatatattt gtatatattt
     4561 ccctcacagc tatatttatt ctaatttaat attatgactt tttaaggtaa ttttttgtga
     4621 cctgttcgga gtgattagcg ttacaatttg aactgaaagt gacatccagt gtttgttcct
     4681 tgtgtagatg catctcaaaa aaatggtggg cataatagtg ttgtttatat atatcaaaaa
     4741 taacaactat aataataaga atacatttaa tttagaaaat gcttggattt cactggaact
     4801 aggctaggat cccacggccg cttggaggga gattagcaaa acgcagcgcg tgatcaaggt
     4861 gacaatgaag ccaccgacca ccacggtggc gcccaacaag gcacgagtgg tttgcatgtc
     4921 cgacacgcac tccctgacgc cctacatcaa gttcgatata ccagatggcg atatattcat
     4981 acatgccggc gattttacca agtgcggcca actggaggag gtggaggagt ttaacacctg
     5041 gattggagca ctgccccatc gccacaagat cgtgattgct ggcaatcatg agctcagctt
     5101 cgatagaacc ttcacccatc ctttccagaa gagcaaaagt catgcttcga gcagcaaaca
     5161 caccggcatg tccatcctcg atgatctgcc cactttgggc aacgccaagg agaacttgga
     5221 gagcgctgtg caaacgcaga atgtccgcga tgttctgacc aactgtcggt atttggagga
     5281 tgaactactt gagatttggg gtattcaaat atatggctcg ccatggcaac cggaattctg
     5341 tcgctgggcc tttaatgttc cacgtggaac tgcctgtttg gataagtgga atcaaatccc
     5401 ggaaggcatc gatatcctgg tcacccacac gccgcccgtg ggtcacggcg atctctgctg
     5461 ttccggcgta cgagccggtt gtgtggagct actcagcaca gtgcagcaac gagtgcgccc
     5521 gaaataccat gtcttcggac atgtacacga aggctacggc atcaccagcg atggcaggat
     5581 catctttgtg aatgcctcaa cgtgtgacat caactaccta cccaacaatg cccccatcgt
     5641 tttcgatgtg acgctgcccc ccggatttcg caaggattag catgcctaga tgattattta
     5701 ttgtgtcaca ggttcaatga atttattttc gtcccacttt atgtttatgt taaggtattt
     5761 cgtctgtaaa gattttgaag cacaattgta ggtgtctaga gagatagaat tcaattcgca
     5821 cgaaattaaa aaaagaaatt cacacttaag atgccataaa atatttattc aagtagagtg
     5881 catttgtcaa tatatgaatt tgtaaaaata aacaagtttt gaagacacct aaacttttga
     5941 agattttcaa tatgatatta tatagttttt aataataatt taagtaaagg taaagcaaat
     6001 cctgagatac atacatacta tttcaaatta gtatcgataa gccgccagaa atgggttgga
     6061 aaaaccttgg aatgattatt ggttagcagc cctgtctccc aatttgacat tcctctttgg
     6121 cgctctgtaa cggcgctttt ccagtgactc cccaatcagc gaaccaacta acctgaggct
     6181 gcacttttcg cgtggcgacc aaaaaaatta taaaaaaaag gtcccgcatt agattttaat
     6241 ttgtatacac ccaattttag tccagcaaaa aaaaactaat caattggtga gaagaaggaa
     6301 aaactaatta gtttaagatt gctgaaagcg ggaagtgaat ttaaatcaga aaagatgtgt
     6361 aagaagatgt gaaatgaaaa ggtgtgggaa gtgaacatga ataaatgttc attgaaaatg
     6421 aacagctaat tgcgaatttt ttgaaactta actgttaact gttactgctc acaaatcctt
     6481 gaaaaatatt aagatcgatc ttagaattgt ttatttaaaa tgaattcctc gtatgtatta
     6541 taacagacaa tcaagtttat taaagaatat ttaaacttgt attttttttt gagtttgata
     6601 aaatgtccca aatataaaag agtattacct tttcgtcata taatactgtt cttaatatta
     6661 gttttagtta gtaatgtgtt tataaagtaa attacaaagg gaggacctaa cctaacctta
     6721 aacaatggcg ccttctcttg cccagctcat cacttttatt gtttcacccg ccagcgcgaa
     6781 gtagtgtcca tccagattgg gacctgcagg gtaccaacat gagtaaagga gaagaacttt
     6841 tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt
     6901 ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt
     6961 gcactactgg aaaactacct gttccatggc caaccctggt caccaccctg acctacggcg
     7021 tgcagtgctt ctcccgttac cctgatcata tgaagcggca cgacttcttc aagagcgcca
     7081 tgcctgaggg atacgtgcag gagaggacca tcttcttcaa ggacgacggg aactacaaga
     7141 cacgtgctga agtcaagttt gagggagaca ccctcgtcaa caggatcgag cttaagggaa
     7201 tcgatttcaa ggaggacgga aacatcctcg gccacaagtt ggaatacaac tacaactccc
     7261 acaacgtata catcatggcc gacaagcaaa agaacggcat caaagccaac ttcaagaccc
     7321 gccacaacat cgaagacggc ggcgtgcaac tcgctgatca ttatcaacaa aatactccaa
     7381 ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa tctgccctag
     7441 atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta
     7501 cacatggcat ggatgaacta tacaaactcg aggtcgacgg tatcgataag cttgatatcg
     7561 aattcctgca gcccggggga tcccatatga gatctaggcc tactagttct agagcctgca
     7621 gcccggggga tcccatatga gatctaggcc tactagttct agagcggccg ctaagtagct
     7681 agtctcgaga ggaattcgag ctcgcccact aagcgtcgcg ccacttcaac gctcgatggg
     7741 agcgtcattg gtgggcgggg taaccgtcga aatcagtgtt tacgcttcca atcgcaacaa
     7801 aaaattcact gcaacactga aaagcatacg aaaacgatga agattgtacg agaaaccata
     7861 aagtatttta tccacaaaga cacgtatagc agaaaagcca agttgggcga gctcgaattc
     7921 cttgcagcca agctgatcca ctagtgtcga cgatgtaggt cacggtctcg aagccgcggt
     7981 gcgggtgcca gggcgtgccc ttgggctccc cgggcgcgta ctccacctca cccatctggt
     8041 ccatcatgat gaacgggtcg aggtggcggt agttgatccc ggcgaacgcg cggcgcaccg
     8101 ggaagccctc gccctcgaaa ccgctgggcg cggtggtcac ggtgagcacg ggacgtgcga
     8161 cggcgtcggc gggtgcggat acgcggggca gcgtcagcgg gttctcgacg gtcacggcgg
     8221 gcatgtcgac actagttcta gccagctttt gttcccttta gtgagggtta atttcgagct
     8281 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     8341 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     8401 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     8461 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     8521 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     8581 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     8641 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     8701 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     8761 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     8821 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     8881 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     8941 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     9001 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     9061 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     9121 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     9181 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     9241 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     9301 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     9361 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     9421 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     9481 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     9541 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     9601 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     9661 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     9721 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     9781 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     9841 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     9901 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     9961 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
    10021 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
    10081 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    10141 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    10201 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    10261 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    10321 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    10381 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    10441 cac
//
