LOCUS       40924_45698        4657 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pUK-S, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4657)
  AUTHORS   Krieg AM, Wu T, Weeratna R, Efler SM, Love-Homan L, Yang L, Yi AK, 
            Short D, Davis HL.
  TITLE     Sequence motifs in adenoviral DNA block immune activation by 
            stimulatory CpG motifs
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 95 (21), 12631-12636 (1998)
  PUBMED    9770537
REFERENCE   2  (bases 1 to 4657)
  AUTHORS   Wu T, Efler SM, Davis HL, Krieg AM, Schorr J.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-1998) HGD, Loeb Health Research Institute, 725 
            Parkdale Ave., Ottawa, ON K1Y 4E9, Canada
REFERENCE   3  (bases 1 to 4657)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4657)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "1998"; volume: "95"; issue: "21"; pages: 
            "12631-12636"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-MAR-1998) HGD, Loeb Health Research Institute, 725 Parkdale 
            Ave., Ottawa, ON K1Y 4E9, Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4657
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    77..284
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(312..328)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(336..352)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    complement(407..428)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(716..1304)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1450..2256)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3202..3218
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3225..3243
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3258..3274)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        3303..3682
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3683..3886
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             3958..4635
                     /codon_start=1
                     /label=HBsAg (S)
                     /note="major/small (S) envelope protein of hepatitis B
                     virus"
                     /translation="MENITSGFLGPLLVLQAGFFLLTRILTIPQSLDSWWTSLNFLGGT
                     TVCLGQNSQSPTSNHSPTSCPPTCPGYRWMCLRRFIIFLFILLLCLIFLLVLLDYQGML
                     PVCPLIPGSSTTSTGPCRTCMTTAQGTSMYPSCCCTKPSDGNCTCIPIPSSWAFGKFLW
                     EWASARFSWLSLLVPFVQWFVGLSPTVWLSVIWMMWYWGPSLYSILSPFLPLLPIFFCL
                     WVYI"
ORIGIN
        1 gaattcgagc tcccgggtac catggcatgc atcgatagat ctcgagtcta gactagagct
       61 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
      121 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
      181 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
      241 agcaaggggg aggattggga agacaatagc aggcatgctg gggaaggcct cggactagtg
      301 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
      361 aacatacgag ccgcggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
      421 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
      481 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
      541 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
      601 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
      661 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
      721 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
      781 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
      841 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
      901 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
      961 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     1021 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     1081 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     1141 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     1201 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     1261 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     1321 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     1381 gcttgcgccg tcccgtcaag tcagcgtaat gctctgccag tgttacaacc aattaaccaa
     1441 ttctgattag aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt
     1501 atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca
     1561 gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat
     1621 acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt
     1681 gacgactgaa tccggtgaga atggcaaaag tttatgcatt tctttccaga cttgttcaac
     1741 aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg
     1801 tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta aaaggacaat tacaaacagg
     1861 aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc
     1921 aggatattct tctaatacct ggaatgctgt ttttccgggg atcgcagtgg tgagtaacca
     1981 tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag
     2041 ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt
     2101 cagaaacaac tctggcgcat cgggcttccc atacaagcga tagattgtcg cacctgattg
     2161 cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa
     2221 tcgcggcctc gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat
     2281 gtaagcagac agttttattg ttcatgatga tatattttta tcttgtgcaa tgtaacatca
     2341 gagattttga gacacaacgt ggctttcccc ccccccccca tgacattaac ctataaaaat
     2401 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
     2461 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     2521 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
     2581 tcagagcaga ttgtactgag agtgcaccat aaaattgtaa acgttaatat tttgttaaaa
     2641 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aatagaccga aatcggcaaa
     2701 atcccttata aatcaaaaga atagcccgag atagagttga gtgttgttcc agtttggaac
     2761 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     2821 ggcgatggcc caccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     2881 gaagggaaga aagcgaaagg agcgggcgct aaggcgctgg caagtgtagc ggtcacgctg
     2941 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgta ctatggttgc
     3001 tttgacgtat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     3061 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     3121 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     3181 ggttttccca gtcacgacgt tgtaaaacga cggccagtga attgtaatac gactcactat
     3241 agggcgaatt ggggatcgat ccactagttc tagatccgat gtacgggcca gatatacgcg
     3301 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
     3361 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     3421 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     3481 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     3541 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     3601 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     3661 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     3721 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     3781 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     3841 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag
     3901 agaacccact gcttactggc ttatcgaaat tgcggccgcc acggcgatat cctgaacatg
     3961 gagaacatca catcaggatt cctaggaccc cttctcgtgt tacaggcggg gtttttcttg
     4021 ttgacaagaa tcctcacaat accgcagagt ctagactcgt ggtggacttc tctcaatttt
     4081 ctagggggaa ctaccgtgtg tcttggccaa aattcgcagt ccccaacctc caatcactca
     4141 ccaacctctt gtcctccaac ttgtcctggt tatcgctgga tgtgtctgcg gcgttttatc
     4201 atcttcctct tcatcctgct gctatgcctc atcttcttgt tggttcttct ggactatcaa
     4261 ggtatgttgc ccgtttgtcc tctaattcca ggatcctcaa caaccagcac gggaccatgc
     4321 cggacctgca tgactactgc tcaaggaacc tctatgtatc cctcctgttg ctgtaccaaa
     4381 ccttcggacg gaaattgcac ctgtattccc atcccatcat cctgggcttt cggaaaattc
     4441 ctatgggagt gggcctcagc ccgtttctcc tggctcagtt tactagtgcc atttgttcag
     4501 tggttcgtag ggctttcccc cactgtttgg ctttcagtta tatggatgat gtggtattgg
     4561 gggccaagtc tgtacagcat cttgagtccc tttttaccgc tgttaccaat tttcttttgt
     4621 ctttgggtat acatttaaac cctactgcag aagcttc
//
