LOCUS       40924_45478        3850 bp DNA     circular SYN 18-DEC-2018
DEFINITION  PCR template vector pUG27, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Gueldener U, Heinisch J, Koehler GJ, Voss D, Hegemann JH.
  TITLE     A second set of loxP marker cassettes for Cre-mediated multiple gene
            knockouts in budding yeast
  JOURNAL   Nucleic Acids Res. 30 (6), E23 (2002)
  PUBMED    11884642
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Gueldener U, Heinisch JH, Voss D, Koehler G, Hegemann JH.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2000) Institut fuer Mikrobiologie, 
            Heinrich-Heine-Universitaet, Universtitaetsstr. 1, Duesseldorf 
            40225, Germany
REFERENCE   3  (bases 1 to 3850)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3850)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2002"; volume: "30"; issue: "6"; pages: "E23"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (23-AUG-2000) Institut fuer Mikrobiologie, 
            Heinrich-Heine-Universitaet, Universtitaetsstr. 1, Duesseldorf 
            40225, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3850
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    53..86
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     gene            141..1341
                     /label=HIS3MX6
                     /note="yeast selectable marker encoding the S. pombe his5
                     gene, which corresponds to S. cerevisiae HIS3"
     misc_feature    1401..1434
                     /label=loxP site
                     /note="loxP site"
     protein_bind    complement(1401..1434)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     promoter        complement(1488..1506)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(1764..2352)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2526..3383)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3384..3488)
                     /label=AmpR promoter
     promoter        3834..3850
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acaaccctta atataacttc
       61 gtataatgta tgctatacga agttattagg tctagagatc tgtttagctt gcctcgtccc
      121 cgccgggtca cccggccagc gacatggagg cccagaatac cctccttgac agtcttgacg
      181 tgcgcagctc aggggcatga tgtgactgtc gcccgtacat ttagcccata catccccatg
      241 tataatcatt tgcatccata cattttgatg gccgcacggc gcgaagcaaa aattacggct
      301 cctcgctgca gacctgcgag cagggaaacg ctcccctcac agacgcgttg aattgtcccc
      361 acgccgcgcc cctgtagaga aatataaaag gttaggattt gccactgagg ttcttctttc
      421 atatacttcc ttttaaaatc ttgctaggat acagttctca catcacatcc gaacataaac
      481 aaccatgggt aggagggctt ttgtagaaag aaatacgaac gaaacgaaaa tcagcgttgc
      541 catcgctttg gacaaagctc ccttacctga agagtcgaat tttattgatg aacttataac
      601 ttccaagcat gcaaaccaaa agggagaaca agtaatccaa gtagacacgg gaattggatt
      661 cttggatcac atgtatcatg cactggctaa acatgcaggc tggagcttac gactttactc
      721 aagaggtgat ttaatcatcg atgatcatca cactgcagaa gatactgcta ttgcacttgg
      781 tattgcattc aagcaggcta tgggtaactt tgccggcgtt aaaagatttg gacatgctta
      841 ttgtccactt gacgaagctc tttctagaag cgtagttgac ttgtcgggac ggccctatgc
      901 tgttatcgat ttgggattaa agcgtgaaaa ggttggggaa ttgtcctgtg aaatgatccc
      961 tcacttacta tattcctttt cggtagcagc tggaattact ttgcatgtta cctgcttata
     1021 tggtagtaat gaccatcatc gtgctgaaag cgcttttaaa tctctggctg ttgccatgcg
     1081 cgcggctact agtcttactg gaagttctga agtcccaagc acgaagggag tgttgtaaag
     1141 agtactgaca ataaaaagat tcttgttttc aagaacttgt catttgtata gtttttttat
     1201 attgtagttg ttctatttta atcaaatgtt agcgtgattt atattttttt tcgcctcgac
     1261 atcatctgcc cagatgcgaa gttaagtgcg cagaaagtaa tatcatgcgt caatcgtatg
     1321 tgaatgctgg tcgctatact gctgtcgatt cgatactaac gccgccatcc agtttaaacg
     1381 agctctcgag aacccttaat ataacttcgt ataatgtatg ctatacgaag ttattaggtg
     1441 atatcagatc cactagtggc ctatgcggcc gcggatctgc cggtctccct atagtgagtc
     1501 gtattaattt cgataagcca ggttaacctg cattaatgaa tcggccaacg cgcggggaga
     1561 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     1621 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     1681 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     1741 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     1801 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1861 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     1921 tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc
     1981 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     2041 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     2101 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2161 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     2221 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     2281 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     2341 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     2401 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     2461 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     2521 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     2581 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     2641 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     2701 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     2761 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     2821 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     2881 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     2941 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     3001 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     3061 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     3121 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     3181 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     3241 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     3301 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     3361 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     3421 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     3481 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     3541 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
     3601 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     3661 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     3721 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat ggacatattg
     3781 tcgttagaac gcggctacaa ttaatacata accttatgta tcatacacat acgatttagg
     3841 tgacactata
//
