LOCUS       40924_45463       11766 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pUDE215, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11766)
  AUTHORS   Kozak BU, van Rossum HM, Benjamin KR, Wu L, Daran JM, Pronk JT, van 
            Maris AJ.
  TITLE     Replacement of the Saccharomyces cerevisiae acetyl-CoA synthetases 
            by alternative pathways for cytosolic acetyl-CoA synthesis
  JOURNAL   Metab. Eng. (2013) In press
  PUBMED    24269999
REFERENCE   2  (bases 1 to 11766)
  AUTHORS   Kozak BU, van Rossum HM, Benjamin KR, Wu L, Daran J-M.G., Pronk JT, 
            van Maris AJA.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAY-2013) Biotechnology, Delft University of 
            Technology, Julianalaan 67, Delft 2628BC, The Netherlands
REFERENCE   3  (bases 1 to 11766)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11766)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab. Eng.
            (2013) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-MAY-2013) Biotechnology, Delft University of Technology, 
            Julianalaan 67, Delft 2628BC, The Netherlands"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: IDBA v. 0.20
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11766
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(183..771)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(945..1802)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(1803..1907)
                     /label=AmpR promoter
     rep_origin      complement(1934..3276)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        3538..3753
                     /label=URA3 promoter
     CDS             3754..4554
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     rep_origin      complement(4688..5143)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     5284..5300
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5310..5328
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        5838..6357
                     /label=TPI1 promoter
                     /note="strong constitutive promoter for yeast triose
                     phosphate isomerase"
     CDS             6358..8646
                     /codon_start=1
                     /gene="PFL"
                     /product="pyruvate formate lyase"
                     /label=PFL
                     /protein_id="AHB33291.1"
                     /translation="MTLKASKKAAVPVMQAKTYATAPVITNDAAAKSEIDVEGWIKKHY
                     TPYEGDGSFLAGPTEKTKKLFAKAEEYLAKERANGGLYDVDPHTPSTITSHKPGYLDKE
                     NEVIYGYQTDVPLKRAIKPFGGVNMVKNALKAVNVPMDKEVEHIFSDYRKTHNTAVFDI
                     YSKEMRAGRSNAIMTGLPDGYGRGRIIGDYRRVALYGTDRLIAQKEKDKAELQKKQMDE
                     PTMKLIGEVADQVKALKQLTQMAKSYGIDITKPAKNAREATQFVYFGYLGSIKEQDGAA
                     MSLGRVDAFLDCFFENDLKNGVITESEAQEIIDNLILKLRFARHLRTPEYNDLFAGDPT
                     WVTMSLGGMGSDGRTLVTKTSFRVLNTLYNLGPAPEPNITVLWNKNLPKNFKDFATKVS
                     IDTSSIQYESDALMSARFGDDYGIACCVSAMRIGKDMQFFGARCNLAKLMLYVLNHGKD
                     ERTGKQVGPDFGPVPEGPIPFDWMWETYDKAMDWIAKLYVNTMNVIHFCHDQYCYESLQ
                     MALHDTDVRRLMAFGVAGLSVVADSFSAIKYAKVTPIRDPKTGLTVDFKVEGEFPKFGN
                     DDDRVDFFARTVTDKLINKLRKTPTYRGATHTLSILTITSNVVYGKKTGSTPDGRKAGQ
                     PFAPGCNPMHGREFSGAVASLSSVAKVNYDSCMDGISNTFSIVPNTIGKTLQERQGNLS
                     GLLDGYFSKGAHHLNVNVLKRETLEDAMAHPENYPNLTIRVSGYAVNFVKLTPAQQKEV
                     IARTFHEKM"
     gene            6358..8646
                     /gene="PFL"
                     /label=PFL
     regulatory      8647..9147
                     /label=GND2 terminator
                     /note="GND2 terminator"
                     /regulatory_class="terminator"
     regulatory      9152..10152
                     /label=FBA1 promoter
                     /note="FBA1 promoter"
                     /regulatory_class="promoter"
     CDS             10153..10953
                     /codon_start=1
                     /gene="PFL-AE"
                     /product="pyruvate formate lyase activating enzyme"
                     /label=PFL-AE
                     /protein_id="AHB33292.1"
                     /translation="MPAIVDPTTMDYMEVKGNVHSTESLACLEGPGNRFLLFLNGCAAR
                     CLYCSNPDTWDETVGTPMTVGQLIKKIGNLKNYYINSVGGGGVTVSGGEPLTQFGFLSC
                     FLYAVKKHLNLHTCVETTGQGCTKAWNSVLPHTDLCLVCIKHAIPEKYEQITRTKKLDR
                     CLKFLKELEKRNIPWWCRYVVLPGYTDSKEDIEALIELVKNSPTCERIEFLPYPELGKN
                     KWEELGIEYPLKNIKQLKKSEIKWICDMVREAFKDRNIPVTGDT"
     gene            10153..10953
                     /gene="PFL-AE"
                     /label=PFL-AE
     regulatory      10954..11454
                     /label=PMA1 terminator
                     /note="PMA1 terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(11455..11471)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(11508..11526)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11547..11563)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    11571..11587
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(11595..11625)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(11640..11661)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
ORIGIN
        1 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
       61 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
      121 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      181 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      241 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      301 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      361 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      421 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
      481 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
      541 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
      601 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
      661 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
      721 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
      781 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
      841 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
      901 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
      961 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     1021 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     1081 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     1141 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     1201 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     1261 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     1321 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     1381 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     1441 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     1501 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     1561 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     1621 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     1681 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     1741 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     1801 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     1861 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     1921 aaagtgccac ctgaacgaag catctgtgct tcattttgta gaacaaaaat gcaacgcgag
     1981 agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc
     2041 gaaagcgcta ttttaccaac gaagaatctg tgcttcattt ttgtaaaaca aaaatgcaac
     2101 gcgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc
     2161 aacgcgagag cgctatttta ccaacaaaga atctatactt cttttttgtt ctacaaaaat
     2221 gcatcccgag agcgctattt ttctaacaaa gcatcttaga ttactttttt tctcctttgt
     2281 gcgctctata atgcagtctc ttgataactt tttgcactgt aggtccgtta aggttagaag
     2341 aaggctactt tggtgtctat tttctcttcc ataaaaaaag cctgactcca cttcccgcgt
     2401 ttactgatta ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat ccccgattat
     2461 attctatacc gatgtggatt gcgcatactt tgtgaacaga aagtgatagc gttgatgatt
     2521 cttcattggt cagaaaatta tgaacggttt cttctatttt gtctctatat actacgtata
     2581 ggaaatgttt acattttcgt attgttttcg attcactcta tgaatagttc ttactacaat
     2641 ttttttgtct aaagagtaat actagagata aacataaaaa atgtagaggt cgagtttaga
     2701 tgcaagttca aggagcgaaa ggtggatggg taggttatat agggatatag cacagagata
     2761 tatagcaaag agatactttt gagcaatgtt tgtggaagcg gtattcgcaa tattttagta
     2821 gctcgttaca gtccggtgcg tttttggttt tttgaaagtg cgtcttcaga gcgcttttgg
     2881 ttttcaaaag cgctctgaag ttcctatact ttctagagaa taggaacttc ggaataggaa
     2941 cttcaaagcg tttccgaaaa cgagcgcttc cgaaaatgca acgcgagctg cgcacataca
     3001 gctcactgtt cacgtcgcac ctatatctgc gtgttgcctg tatatatata tacatgagaa
     3061 gaacggcata gtgcgtgttt atgcttaaat gcgtacttat atgcgtctat ttatgtagga
     3121 tgaaaggtag tctagtacct cctgtgatat tatcccattc catgcggggt atcgtatgct
     3181 tccttcagca ctacccttta gctgttctat atgctgccac tcctcaattg gattagtctc
     3241 atccttcaat gctatcattt cctttgatat tggatcatac taagaaacca ttattatcat
     3301 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
     3361 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     3421 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     3481 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata ccaccttttc
     3541 aattcatcat ttttttttta ttcttttttt tgatttcggt ttccttgaaa tttttttgat
     3601 tcggtaatct ccgaacagaa ggaagaacga aggaaggagc acagacttag attggtatat
     3661 atacgcatat gtagtgttga agaaacatga aattgcccag tattcttaac ccaactgcac
     3721 agaacaaaaa cctgcaggaa acgaagataa atcatgtcga aagctacata taaggaacgt
     3781 gctgctactc atcctagtcc tgttgctgcc aagctattta atatcatgca cgaaaagcaa
     3841 acaaacttgt gtgcttcatt ggatgttcgt accaccaagg aattactgga gttagttgaa
     3901 gcattaggtc ccaaaatttg tttactaaaa acacatgtgg atatcttgac tgatttttcc
     3961 atggagggca cagttaagcc gctaaaggca ttatccgcca agtacaattt tttactcttc
     4021 gaagacagaa aatttgctga cattggtaat acagtcaaat tgcagtactc tgcgggtgta
     4081 tacagaatag cagaatgggc agacattacg aatgcacacg gtgtggtggg cccaggtatt
     4141 gttagcggtt tgaagcaggc ggcagaagaa gtaacaaagg aacctagagg ccttttgatg
     4201 ttagcagaat tgtcatgcaa gggctcccta tctactggag aatatactaa gggtactgtt
     4261 gacattgcga agagcgacaa agattttgtt atcggcttta ttgctcaaag agacatgggt
     4321 ggaagagatg aaggttacga ttggttgatt atgacacccg gtgtgggttt agatgacaag
     4381 ggagacgcat tgggtcaaca gtatagaacc gtggatgatg tggtctctac aggatctgac
     4441 attattattg ttggaagagg actatttgca aagggaaggg atgctaaggt agagggtgaa
     4501 cgttacagaa aagcaggctg ggaagcatat ttgagaagat gcggccagca aaactaaaaa
     4561 actgtattat aagtaaatgc atgtatacta aactcacaaa ttagagcttc aatttaatta
     4621 tatcagttat taccctatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     4681 catcaggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     4741 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     4801 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     4861 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     4921 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     4981 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     5041 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     5101 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtccattcg ccattcaggc
     5161 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     5221 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     5281 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
     5341 ccgggccccc cctcgagaag gatgagccaa gaataaggga acaagatttt ggtaatttcc
     5401 aaaaaatcaa tagcatgcag gacgttatga agaagagatc tacgtatggt catttcttct
     5461 tcagattccc tcatggagaa agtgcggcag atgtatatga cagagtcgcc agtttccaag
     5521 agactttatt caggcacttc catgataggc aagagagaag acccagagat gttgttgtcc
     5581 tagttacaca tggtatttat tccagagtat tcctgatgaa atggtttaga tggacatacg
     5641 aagagtttga atcgtttacc aatgttccta acgggagcgt aatggtgatg gaactggacg
     5701 aatccatcaa tagatacgtc ctgaggaccg tgctacccaa atggactgat tgtgagggag
     5761 acctaactac atagtgttta aagattacgg atatttaact tacttagaat aatgccattt
     5821 ttttgagtta taataatcct acgttagtgt gagcgggatt taaactgtga ggaccttaat
     5881 acattcagac acttctgcgg tatcacccta cttattccct tcgagattat atctaggaac
     5941 ccatcaggtt ggtggaagat tacccgttct aagacttttc agcttcctct attgatgtta
     6001 cacctggaca ccccttttct ggcatccagt ttttaatctt cagtggcatg tgagattctc
     6061 cgaaattaat taaagcaatc acacaattct ctcggatacc acctcggttg aaactgacag
     6121 gtggtttgtt acgcatgcta atgcaaagga gcctatatac ctttggctcg gctgctgtaa
     6181 cagggaatat aaagggcagc ataatttagg agtttagtga acttgcaaca tttactattt
     6241 tcccttctta cgtaaatatt tttcttttta attctaaatc aatctttttc aattttttgt
     6301 ttgtattctt ttcttgctta aatctataac tacaaaaaac acatacataa actaaaaatg
     6361 actttgaagg cttccaagaa ggctgctgtt ccagttatgc aagccaagac ctacgctact
     6421 gctccagtta tcaccaacga tgctgctgcc aaatctgaaa tcgatgtcga aggttggatc
     6481 aagaagcatt acactccata cgaaggtgat ggttctttct tggccggtcc aactgaaaag
     6541 accaagaaat tattcgccaa ggctgaagaa tacttggcca aggaaagagc caacggtggt
     6601 ttatacgatg tcgaccctca cactccatct accatcactt cccacaagcc tggttacttg
     6661 gacaaggaaa acgaagttat ctacggttac caaaccgatg ttccattgaa gagagccatc
     6721 aagccattcg gtggtgtcaa catggtcaag aatgctttga aggctgttaa cgttccaatg
     6781 gacaaggaag ttgaacacat cttctctgac tacagaaaga cccacaacac tgctgtcttt
     6841 gacatctact ccaaggaaat gagagctggt cgttccaatg ctatcatgac cggtttacca
     6901 gatggttacg gtagaggtag aattatcggt gactacagaa gagttgcttt gtacggtact
     6961 gacagattga ttgctcaaaa ggaaaaggac aaggctgaat tgcaaaagaa gcaaatggac
     7021 gaaccaacta tgaaattgat tggtgaagtt gccgaccaag tcaaggcttt gaagcaattg
     7081 acccaaatgg ctaaatctta cggtattgac atcaccaagc cagccaagaa cgctcgtgaa
     7141 gctacccaat tcgtctactt cggttacttg ggttccatca aggaacaaga tggtgctgct
     7201 atgtccttgg gtcgtgtcga tgccttcttg gactgtttct tcgaaaacga tttgaagaac
     7261 ggtgtcatca ctgaatctga agctcaagaa atcattgaca acttgatttt gaaattgaga
     7321 tttgctcgtc acttgagaac cccagaatac aatgacttgt tcgctggtga cccaacctgg
     7381 gttaccatgt ctttaggtgg tatgggttct gatggtagaa ctttagtcac caagacttct
     7441 ttcagagtct tgaacacttt gtacaactta ggtccagctc cagaaccaaa cattactgtt
     7501 ttgtggaaca agaacttgcc aaagaacttc aaggatttcg ctaccaaggt ttccattgac
     7561 acttcttcta tccaatacga atctgatgct ttgatgtccg ctcgtttcgg tgatgactac
     7621 ggtattgcct gttgtgtttc tgctatgaga attggtaagg acatgcaatt cttcggtgcc
     7681 agatgtaact tggccaaatt gatgttgtac gtcttaaacc acggtaagga tgaaagaact
     7741 ggtaagcaag ttggtccaga tttcggtcct gttccagaag gtccaattcc attcgactgg
     7801 atgtgggaaa cttacgacaa ggctatggac tggattgcca agttgtacgt caacaccatg
     7861 aacgttatcc atttctgtca cgaccaatac tgttacgaat ctttgcaaat ggctttgcac
     7921 gacaccgatg tcagaagatt gatggctttc ggtgttgctg gtttgtccgt tgtcgctgac
     7981 tctttctccg ctatcaaata cgccaaggtc actccaatca gagatccaaa gaccggtttg
     8041 accgttgact tcaaggtcga aggtgaattt ccaaagttcg gtaacgatga cgacagagtc
     8101 gacttcttcg ctagaaccgt cactgacaaa ttgatcaaca aattgagaaa gaccccaacc
     8161 tacagaggtg ctacccacac tttgtctatt ttgactatca cttctaacgt tgtctacggt
     8221 aagaaaaccg gttctactcc agatggtaga aaggctggtc aaccattcgc tccaggttgt
     8281 aacccaatgc acggtagaga attttccggt gctgttgctt ctttgtcctc tgttgctaag
     8341 gttaactatg actcttgtat ggacggtatc tccaacactt tctccattgt cccaaacacc
     8401 attggtaaga ctctacaaga aagacaaggt aacttatctg gtctattaga tggttacttc
     8461 tccaagggtg ctcaccactt gaacgttaac gttttgaaga gagaaacttt ggaagatgct
     8521 atggctcatc cagaaaacta cccaaacttg accatcagag tttctggtta cgctgtcaac
     8581 tttgtcaaat tgaccccagc tcaacaaaag gaagttatcg ccagaacttt ccacgagaaa
     8641 atgtaaggag ttaaaggcaa agttttcttt tctagagccg ttcccacaaa taattatacg
     8701 tatatgcttc ttttcgttta ctatatatct atatttacaa gcctttattc actgatgcaa
     8761 tttgtttcca aatacttttt tggagatctc ataactagat atgatgatgg cgcaacttgg
     8821 gcgtatctta attactctgg ctgccaggcc cgtgtagagg gccgcaagac cttctgtacg
     8881 ccatatagtc tctaagaact tgaacatgtt tctagaccta ttgccgcctt tcggatcgct
     8941 attgttcatc atggatattt gccatctcgt cttaccgaca tcaaaagggt gtgtgcatat
     9001 agcagctatc atcccactta tgcaaccact ggcaaaactg tttataaaat ggacccagtt
     9061 tgcgtcctta gatgcaaatc gagtagaatc tagccatagt ctttccttgc aaagttcata
     9121 ggaactccaa tatattgcac taaacggaat tcactggtag agagcgactt tgtatgcccc
     9181 aattgcgaaa cccgcgttat ccttctcgat tctttagtac ccgaccagga caaggaaaag
     9241 gaggtcgaaa cgtttttgaa gaaacaagag gaactacacg gaagctctaa agatggcaac
     9301 cagccagaaa ctaagaaaat gaagttgatg gttccaactg gcaccgctgg cttgaacaac
     9361 aataccagcc ttccaacttc tgtaaataac ggcggtacgc cagtgccacc agtaccgtta
     9421 cctttcggta tacctccttt ccccatgttt ccaatgccct tcatgcctcc aacggctact
     9481 atcacaaatc ctcatcaagc tgacgcaagc cctaagaaat gaataacaat actgacagta
     9541 ctaaataatt gcctacttgg cttcacatac gttgcatacg tcgatataga taataatgat
     9601 aatgacagca ggattatcgt aatacgtaat agttgaaaat ctcaaaaatg tgtgggtcat
     9661 tacgtaaata atgataggaa tgggattctt ctatttttcc tttttccatt ctagcagccg
     9721 tcgggaaaac gtggcatcct ctctttcggg ctcaattgga gtcacgctgc cgtgagcatc
     9781 ctctctttcc atatctaaca actgagcacg taaccaatgg aaaagcatga gcttagcgtt
     9841 gctccaaaaa agtattggat ggttaatacc atttgtctgt tctcttctga ctttgactcc
     9901 tcaaaaaaaa aaaatctaca atcaacagat cgcttcaatt acgccctcac aaaaactttt
     9961 ttccttcttc ttcgcccacg ttaaatttta tccctcatgt tgtctaacgg atttctgcac
    10021 ttgatttatt ataaaaagac aaagacataa tacttctcta tcaatttcag ttattgttct
    10081 tccttgcgtt attcttctgt tcttcttttt cttttgtcat atataaccat aaccaagtaa
    10141 tacatattca aaatgccagc cattgtcgac ccaactacca tggactacat ggaagtcaag
    10201 ggtaacgtcc actccactga atctttggct tgtttggaag gtccaggtaa cagattctta
    10261 ttattcttga atggttgtgc tgctagatgt ttgtactgtt ccaacccaga cacctgggat
    10321 gaaaccgttg gtactccaat gaccgtcggt caattgatca agaagatcgg taacttgaag
    10381 aactactaca tcaactctgt tggtggtggt ggtgtcactg tttccggtgg tgaaccattg
    10441 actcaattcg gtttcttgtc ttgtttctta tacgctgtca agaagcattt gaacttgcac
    10501 acttgtgttg aaaccaccgg tcaaggttgt accaaggctt ggaactctgt tttgcctcac
    10561 actgacttgt gtttggtttg tatcaagcac gccattccag aaaagtacga acaaatcacc
    10621 agaaccaaga aattggatcg ttgtttgaaa ttcttgaagg aattggaaaa gagaaacatt
    10681 ccatggtggt gtcgttacgt tgtcttgcca ggttacaccg attccaagga agatattgaa
    10741 gctttgattg aattagtcaa gaactctcca acttgtgaaa gaatcgaatt tttgccatac
    10801 ccagaattgg gtaagaacaa atgggaagaa ttaggtatcg aatacccatt gaagaacatc
    10861 aagcaattga agaagtctga aatcaaatgg atctgtgaca tggttagaga agctttcaag
    10921 gacagaaaca ttccagttac tggtgacact taagctcctg ttgaagtagc atttaatcat
    10981 aatttttgtc acattttaat caacttgatt tttctggttt aatttttcta attttaattt
    11041 taattttttt atcaatggga actgatacac taaaaagaat taggagccaa caagaataag
    11101 ccgcttattt cctactagag tttgcttaaa atttcatctc gaattgtcat tctaatattt
    11161 tatccacaca cacaccttaa aatttttaga ttaaatggca tcaactctta gcttcacaca
    11221 cacacacaca ccgaagctgg ttgttttatt tgatttgata taattggttt ctctggatgg
    11281 tactttttct ttcttggtta tttcctattt taaaatatga aacgcacaca agtcataatt
    11341 attctaatag agcacaattc acaacacgca catttcaact ttaatatttt tttagaaaca
    11401 ctttatttag tctaattctt aatttttaat atatataatg cacacacacg tttggatcca
    11461 ctagttctag agcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg
    11521 ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
    11581 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
    11641 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
    11701 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
    11761 gggcgc
//
