LOCUS       40924_45458       11757 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pUDE214, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11757)
  AUTHORS   Kozak BU, van Rossum HM, Benjamin KR, Wu L, Daran JM, Pronk JT, van 
            Maris AJ.
  TITLE     Replacement of the Saccharomyces cerevisiae acetyl-CoA synthetases 
            by alternative pathways for cytosolic acetyl-CoA synthesis
  JOURNAL   Metab. Eng. (2013) In press
  PUBMED    24269999
REFERENCE   2  (bases 1 to 11757)
  AUTHORS   Kozak BU, van Rossum HM, Benjamin KR, Wu L, Daran J-M.G., Pronk JT, 
            van Maris AJA.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAY-2013) Biotechnology, Delft University of 
            Technology, Julianalaan 67, Delft 2628BC, The Netherlands
REFERENCE   3  (bases 1 to 11757)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11757)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab. Eng.
            (2013) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-MAY-2013) Biotechnology, Delft University of Technology, 
            Julianalaan 67, Delft 2628BC, The Netherlands"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: IDBA v. 0.20
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11757
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(183..771)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(945..1802)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(1803..1907)
                     /label=AmpR promoter
     rep_origin      complement(1934..3276)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        3538..3753
                     /label=URA3 promoter
     CDS             3754..4554
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     rep_origin      complement(4688..5143)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     5284..5300
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5310..5328
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        5838..6357
                     /label=TPI1 promoter
                     /note="strong constitutive promoter for yeast triose
                     phosphate isomerase"
     CDS             6358..8616
                     /codon_start=1
                     /gene="pflB"
                     /product="formate C-acetyltransferase"
                     /label=pflB
                     /protein_id="AHB33316.1"
                     /translation="MITSEKTTKPAAWKGFKGGHWQEEINIRDFIQNNFTQYNGDESFL
                     AGPTAATKTLNDKVLELKKQERAAGGVLDADTKVVATITSHGPGYIQKDLEKIVGLQTD
                     KPLKRAFMPFGGIRMADDALKSYGYTPDEENDKIFTEYRKTHNQGVFDVYTPDMRKARH
                     YKIITGLPDAYARGRLIPDLPRVAVYGIDRLMEDKANDFAHIGDGELTDDVIRLREEVQ
                     DQYRALADMKKMAASYGYDISKPATNAQEAIQWMYFAYLAAIKTQNGAAMSVGRIDTTM
                     DIFIQRDLDNGVLDESQAQELIDQFVMKLRMVRFIRTEEYNSLFSGDPIWATLSMCGLG
                     VDGQHHVTKTAFRILKTLDNMGAAPEPNITILWSDRLPEDFKRYATEVSIDSSTIQYEN
                     DDLMRVQWGTDYYGIACCVSAQPIADGIQYFGARANLAKAILYAINGGRDEIAGDQVGP
                     AYEPITSEYIDYDEFMKKLDKQMDWLADTYVNSLNAIHYMHDKYYYEAAQLALKNTDLD
                     RTFATGISGLSHAADSISAIKYGHVKVIRDERGIAVDFKADNDYPRYGNNDDRADDIAK
                     WLVKELYSKMNTHHLYRNAKLSTSVLTITSNVVYGKNTGTTPNGRQKGEPFSPGANPAY
                     GAEKSGALASLLSTAKLPYRYATDGISNTFGVTPNTLGHDLESRKDTLVNMLDGYMKND
                     GMHLNINVFNKDTLIDAQKHPEEYPTLTVRVSGYCVYFADLTKEQQDDVISRTFFESM"
     gene            6358..8616
                     /gene="pflB"
                     /label=pflB
     regulatory      8617..9117
                     /label=GND2 terminator
                     /note="GND2 terminator"
                     /regulatory_class="terminator"
     regulatory      9122..10122
                     /label=FBA1 promoter
                     /note="FBA1 promoter"
                     /regulatory_class="promoter"
     CDS             10123..10944
                     /codon_start=1
                     /gene="pflA"
                     /product="formate C-acetyltransferase activating enzyme"
                     /label=pflA
                     /protein_id="AHB33317.1"
                     /translation="MENKQVSTTQAAAKEPLIGYVHSIETFGSVDGPGIRYVAFLQGCH
                     MRCQYCHNPDTWKLNVGDQMTADEILEDAAKYRAFWGKTGGITVSGGESLVQIDFILDL
                     FEKAKAMNISTCLDTSGQPFTREQPFFDKFERLMKVTDISLVDIKHIDSAKHKQLTQYG
                     NENILDMIQYMAQHHDDMWIRHVLVPQRTDYDEDLKKLGDYIAKIPNDVVQKVEVLPYH
                     TLGVKKYHEMKIKYRLEGIESPTQDRVANAEKLLHTADYNGYKTWMPLPKL"
     gene            10123..10944
                     /gene="pflA"
                     /label=pflA
     regulatory      10945..11445
                     /label=PMA1 terminator
                     /note="PMA1 terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(11446..11462)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(11499..11517)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11538..11554)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    11562..11578
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(11586..11616)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(11631..11652)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
ORIGIN
        1 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
       61 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
      121 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      181 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      241 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      301 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      361 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      421 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
      481 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
      541 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
      601 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
      661 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
      721 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
      781 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
      841 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
      901 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
      961 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     1021 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     1081 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     1141 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     1201 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     1261 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     1321 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     1381 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     1441 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     1501 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     1561 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     1621 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     1681 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     1741 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     1801 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     1861 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     1921 aaagtgccac ctgaacgaag catctgtgct tcattttgta gaacaaaaat gcaacgcgag
     1981 agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc
     2041 gaaagcgcta ttttaccaac gaagaatctg tgcttcattt ttgtaaaaca aaaatgcaac
     2101 gcgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc
     2161 aacgcgagag cgctatttta ccaacaaaga atctatactt cttttttgtt ctacaaaaat
     2221 gcatcccgag agcgctattt ttctaacaaa gcatcttaga ttactttttt tctcctttgt
     2281 gcgctctata atgcagtctc ttgataactt tttgcactgt aggtccgtta aggttagaag
     2341 aaggctactt tggtgtctat tttctcttcc ataaaaaaag cctgactcca cttcccgcgt
     2401 ttactgatta ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat ccccgattat
     2461 attctatacc gatgtggatt gcgcatactt tgtgaacaga aagtgatagc gttgatgatt
     2521 cttcattggt cagaaaatta tgaacggttt cttctatttt gtctctatat actacgtata
     2581 ggaaatgttt acattttcgt attgttttcg attcactcta tgaatagttc ttactacaat
     2641 ttttttgtct aaagagtaat actagagata aacataaaaa atgtagaggt cgagtttaga
     2701 tgcaagttca aggagcgaaa ggtggatggg taggttatat agggatatag cacagagata
     2761 tatagcaaag agatactttt gagcaatgtt tgtggaagcg gtattcgcaa tattttagta
     2821 gctcgttaca gtccggtgcg tttttggttt tttgaaagtg cgtcttcaga gcgcttttgg
     2881 ttttcaaaag cgctctgaag ttcctatact ttctagagaa taggaacttc ggaataggaa
     2941 cttcaaagcg tttccgaaaa cgagcgcttc cgaaaatgca acgcgagctg cgcacataca
     3001 gctcactgtt cacgtcgcac ctatatctgc gtgttgcctg tatatatata tacatgagaa
     3061 gaacggcata gtgcgtgttt atgcttaaat gcgtacttat atgcgtctat ttatgtagga
     3121 tgaaaggtag tctagtacct cctgtgatat tatcccattc catgcggggt atcgtatgct
     3181 tccttcagca ctacccttta gctgttctat atgctgccac tcctcaattg gattagtctc
     3241 atccttcaat gctatcattt cctttgatat tggatcatac taagaaacca ttattatcat
     3301 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
     3361 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     3421 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     3481 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata ccaccttttc
     3541 aattcatcat ttttttttta ttcttttttt tgatttcggt ttccttgaaa tttttttgat
     3601 tcggtaatct ccgaacagaa ggaagaacga aggaaggagc acagacttag attggtatat
     3661 atacgcatat gtagtgttga agaaacatga aattgcccag tattcttaac ccaactgcac
     3721 agaacaaaaa cctgcaggaa acgaagataa atcatgtcga aagctacata taaggaacgt
     3781 gctgctactc atcctagtcc tgttgctgcc aagctattta atatcatgca cgaaaagcaa
     3841 acaaacttgt gtgcttcatt ggatgttcgt accaccaagg aattactgga gttagttgaa
     3901 gcattaggtc ccaaaatttg tttactaaaa acacatgtgg atatcttgac tgatttttcc
     3961 atggagggca cagttaagcc gctaaaggca ttatccgcca agtacaattt tttactcttc
     4021 gaagacagaa aatttgctga cattggtaat acagtcaaat tgcagtactc tgcgggtgta
     4081 tacagaatag cagaatgggc agacattacg aatgcacacg gtgtggtggg cccaggtatt
     4141 gttagcggtt tgaagcaggc ggcagaagaa gtaacaaagg aacctagagg ccttttgatg
     4201 ttagcagaat tgtcatgcaa gggctcccta tctactggag aatatactaa gggtactgtt
     4261 gacattgcga agagcgacaa agattttgtt atcggcttta ttgctcaaag agacatgggt
     4321 ggaagagatg aaggttacga ttggttgatt atgacacccg gtgtgggttt agatgacaag
     4381 ggagacgcat tgggtcaaca gtatagaacc gtggatgatg tggtctctac aggatctgac
     4441 attattattg ttggaagagg actatttgca aagggaaggg atgctaaggt agagggtgaa
     4501 cgttacagaa aagcaggctg ggaagcatat ttgagaagat gcggccagca aaactaaaaa
     4561 actgtattat aagtaaatgc atgtatacta aactcacaaa ttagagcttc aatttaatta
     4621 tatcagttat taccctatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     4681 catcaggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     4741 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     4801 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     4861 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     4921 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     4981 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     5041 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     5101 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtccattcg ccattcaggc
     5161 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     5221 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     5281 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
     5341 ccgggccccc cctcgagaag gatgagccaa gaataaggga acaagatttt ggtaatttcc
     5401 aaaaaatcaa tagcatgcag gacgttatga agaagagatc tacgtatggt catttcttct
     5461 tcagattccc tcatggagaa agtgcggcag atgtatatga cagagtcgcc agtttccaag
     5521 agactttatt caggcacttc catgataggc aagagagaag acccagagat gttgttgtcc
     5581 tagttacaca tggtatttat tccagagtat tcctgatgaa atggtttaga tggacatacg
     5641 aagagtttga atcgtttacc aatgttccta acgggagcgt aatggtgatg gaactggacg
     5701 aatccatcaa tagatacgtc ctgaggaccg tgctacccaa atggactgat tgtgagggag
     5761 acctaactac atagtgttta aagattacgg atatttaact tacttagaat aatgccattt
     5821 ttttgagtta taataatcct acgttagtgt gagcgggatt taaactgtga ggaccttaat
     5881 acattcagac acttctgcgg tatcacccta cttattccct tcgagattat atctaggaac
     5941 ccatcaggtt ggtggaagat tacccgttct aagacttttc agcttcctct attgatgtta
     6001 cacctggaca ccccttttct ggcatccagt ttttaatctt cagtggcatg tgagattctc
     6061 cgaaattaat taaagcaatc acacaattct ctcggatacc acctcggttg aaactgacag
     6121 gtggtttgtt acgcatgcta atgcaaagga gcctatatac ctttggctcg gctgctgtaa
     6181 cagggaatat aaagggcagc ataatttagg agtttagtga acttgcaaca tttactattt
     6241 tcccttctta cgtaaatatt tttcttttta attctaaatc aatctttttc aattttttgt
     6301 ttgtattctt ttcttgctta aatctataac tacaaaaaac acatacataa actaaaaatg
     6361 atcacttctg aaaagaccac caagccagcc gcttggaagg gtttcaaggg tggtcactgg
     6421 caagaagaaa tcaacatcag agatttcatc caaaacaact tcacccaata caacggtgac
     6481 gaatctttct tggctggtcc aactgccgct accaagacct tgaacgacaa agttttggaa
     6541 ttgaagaagc aagaaagagc tgctggtggt gtcttggatg ctgacaccaa ggttgtcgcc
     6601 accatcactt ctcacggtcc aggttacatc caaaaggact tggaaaagat cgtcggttta
     6661 caaactgaca agccattgaa gagagctttc atgccattcg gtggtatcag aatggctgat
     6721 gatgctttga aatcttacgg ttacacccca gacgaagaaa acgacaagat cttcaccgaa
     6781 tacagaaaga ctcacaacca aggtgttttc gatgtctaca ctccagacat gagaaaggct
     6841 agacattaca agatcatcac tggtttgcca gacgcttacg ccagaggtag attgattcca
     6901 gatttgccaa gagttgctgt ttacggtatt gacagattga tggaagataa ggctaacgac
     6961 ttcgctcaca ttggtgatgg tgaattgacc gatgatgtca tcagattgcg tgaagaagtt
     7021 caagaccaat acagagcttt ggctgacatg aagaagatgg ctgcttccta cggttacgac
     7081 atctccaagc ctgccaccaa tgctcaagaa gccattcaat ggatgtactt tgcttacttg
     7141 gctgccatca agacccaaaa cggtgctgct atgtctgtcg gtagaattga caccaccatg
     7201 gacattttca tccaaagaga tttggacaac ggtgttttgg acgaatctca agctcaagaa
     7261 ttgattgacc aattcgtcat gaaattgaga atggttagat tcatcagaac tgaagaatac
     7321 aactctttgt tctctggtga cccaatctgg gctactttgt ccatgtgtgg tttaggtgtt
     7381 gacggtcaac accacgttac caagaccgcc ttcagaatct taaagacttt ggacaacatg
     7441 ggtgctgctc cagaaccaaa catcactatc ttgtggtccg acagattacc agaagatttc
     7501 aagagatacg ctactgaagt ctctattgac tcctctacca tccaatacga aaacgatgac
     7561 ttgatgagag tccaatgggg tactgactac tacggtattg cctgttgtgt ctccgctcaa
     7621 ccaattgctg atggtatcca atacttcggt gccagagcta acttggccaa ggctatctta
     7681 tatgctatca acggtggtcg tgatgaaatt gctggtgacc aagttggtcc agcttacgaa
     7741 ccaatcactt ctgaatacat tgactacgat gaatttatga agaaattgga caagcaaatg
     7801 gactggttag ctgacaccta cgtcaactct ttgaatgcca ttcactacat gcatgacaaa
     7861 tactactacg aagctgctca attggctttg aagaacaccg atttggatcg tactttcgct
     7921 actggtattt ctggtctatc ccacgctgct gactctattt ctgccatcaa atacggtcac
     7981 gtcaaggtca tccgtgacga aagaggtatt gctgttgact tcaaggctga taatgactac
     8041 ccaagatacg gtaacaacga tgacagagct gatgacattg ccaaatggtt agtcaaggaa
     8101 ttgtactcca agatgaacac ccatcacttg tacagaaacg ctaaattgtc cacttccgtt
     8161 ttgaccatca cttctaacgt tgtttacggt aagaacaccg gtactacccc aaacggtaga
     8221 caaaagggtg aacctttctc cccaggtgcc aacccagctt acggtgctga aaagtctggt
     8281 gccttggcct ctctattatc tactgctaaa ttgccatacc gttacgctac cgatggtatt
     8341 tccaacacct ttggtgttac tccaaacact ttaggtcacg acttggaatc tagaaaggac
     8401 actttggtta acatgttgga cggttacatg aagaacgatg gtatgcactt gaacatcaac
     8461 gttttcaaca aggacacttt gattgatgct caaaagcacc cagaagaata cccaactttg
     8521 accgtcagag tctctggtta ctgtgtttat ttcgctgatt tgaccaagga acaacaagat
     8581 gatgttatct ccagaacttt ctttgaatcc atgtaaggag ttaaaggcaa agttttcttt
     8641 tctagagccg ttcccacaaa taattatacg tatatgcttc ttttcgttta ctatatatct
     8701 atatttacaa gcctttattc actgatgcaa tttgtttcca aatacttttt tggagatctc
     8761 ataactagat atgatgatgg cgcaacttgg gcgtatctta attactctgg ctgccaggcc
     8821 cgtgtagagg gccgcaagac cttctgtacg ccatatagtc tctaagaact tgaacatgtt
     8881 tctagaccta ttgccgcctt tcggatcgct attgttcatc atggatattt gccatctcgt
     8941 cttaccgaca tcaaaagggt gtgtgcatat agcagctatc atcccactta tgcaaccact
     9001 ggcaaaactg tttataaaat ggacccagtt tgcgtcctta gatgcaaatc gagtagaatc
     9061 tagccatagt ctttccttgc aaagttcata ggaactccaa tatattgcac taaacggaat
     9121 tcactggtag agagcgactt tgtatgcccc aattgcgaaa cccgcgttat ccttctcgat
     9181 tctttagtac ccgaccagga caaggaaaag gaggtcgaaa cgtttttgaa gaaacaagag
     9241 gaactacacg gaagctctaa agatggcaac cagccagaaa ctaagaaaat gaagttgatg
     9301 gttccaactg gcaccgctgg cttgaacaac aataccagcc ttccaacttc tgtaaataac
     9361 ggcggtacgc cagtgccacc agtaccgtta cctttcggta tacctccttt ccccatgttt
     9421 ccaatgccct tcatgcctcc aacggctact atcacaaatc ctcatcaagc tgacgcaagc
     9481 cctaagaaat gaataacaat actgacagta ctaaataatt gcctacttgg cttcacatac
     9541 gttgcatacg tcgatataga taataatgat aatgacagca ggattatcgt aatacgtaat
     9601 agttgaaaat ctcaaaaatg tgtgggtcat tacgtaaata atgataggaa tgggattctt
     9661 ctatttttcc tttttccatt ctagcagccg tcgggaaaac gtggcatcct ctctttcggg
     9721 ctcaattgga gtcacgctgc cgtgagcatc ctctctttcc atatctaaca actgagcacg
     9781 taaccaatgg aaaagcatga gcttagcgtt gctccaaaaa agtattggat ggttaatacc
     9841 atttgtctgt tctcttctga ctttgactcc tcaaaaaaaa aaaatctaca atcaacagat
     9901 cgcttcaatt acgccctcac aaaaactttt ttccttcttc ttcgcccacg ttaaatttta
     9961 tccctcatgt tgtctaacgg atttctgcac ttgatttatt ataaaaagac aaagacataa
    10021 tacttctcta tcaatttcag ttattgttct tccttgcgtt attcttctgt tcttcttttt
    10081 cttttgtcat atataaccat aaccaagtaa tacatattca aaatggaaaa caagcaagtc
    10141 tccaccaccc aagctgctgc caaggaacca ttgattggtt acgttcactc cattgaaact
    10201 ttcggttctg tcgatggtcc aggtatcaga tacgttgctt tcttgcaagg ttgtcacatg
    10261 agatgtcaat actgtcacaa cccagacacc tggaaattga atgtcggtga ccaaatgacc
    10321 gctgatgaaa tcttggaaga tgctgccaag tacagagctt tctggggtaa gaccggtggt
    10381 atcactgttt ccggtggtga atctttggtt caaatcgatt tcatcttgga cttgtttgaa
    10441 aaggccaagg ccatgaacat ctccacctgt ttggacacct ctggtcaacc attcaccaga
    10501 gaacaaccat tcttcgacaa attcgaaaga ttgatgaagg tcactgacat ctctttagtc
    10561 gacatcaagc acattgactc tgctaagcac aagcaattga ctcaatacgg taacgaaaac
    10621 attttggaca tgattcaata catggctcaa catcacgatg acatgtggat cagacacgtt
    10681 ttggttcctc aaagaactga ctacgatgaa gatttgaaga aattgggtga ctacattgct
    10741 aagatcccaa acgatgttgt ccaaaaggtt gaagtcttgc cataccacac tttaggtgtc
    10801 aagaaatacc atgaaatgaa gatcaagtac agattggaag gtattgaatc tccaactcaa
    10861 gatcgtgttg ctaacgctga aaagttgttg cacactgctg actacaacgg ttacaagacc
    10921 tggatgccat taccaaagct ctaagctcct gttgaagtag catttaatca taatttttgt
    10981 cacattttaa tcaacttgat ttttctggtt taatttttct aattttaatt ttaatttttt
    11041 tatcaatggg aactgataca ctaaaaagaa ttaggagcca acaagaataa gccgcttatt
    11101 tcctactaga gtttgcttaa aatttcatct cgaattgtca ttctaatatt ttatccacac
    11161 acacacctta aaatttttag attaaatggc atcaactctt agcttcacac acacacacac
    11221 accgaagctg gttgttttat ttgatttgat ataattggtt tctctggatg gtactttttc
    11281 tttcttggtt atttcctatt ttaaaatatg aaacgcacac aagtcataat tattctaata
    11341 gagcacaatt cacaacacgc acatttcaac tttaatattt ttttagaaac actttattta
    11401 gtctaattct taatttttaa tatatataat gcacacacac gtttggatcc actagttcta
    11461 gagcggccgc caccgcggtg gagctccagc ttttgttccc tttagtgagg gttaattgcg
    11521 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
    11581 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
    11641 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
    11701 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgc
//
