LOCUS       V002341                11697 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002341
VERSION     V002341
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11697)
  AUTHORS   Kozak BU, van Rossum HM, Benjamin KR, Wu L, Daran JM, Pronk JT, van
            Maris AJ.
  TITLE     Replacement of the Saccharomyces cerevisiae acetyl-CoA synthetases
            by alternative pathways for cytosolic acetyl-CoA synthesis
  JOURNAL   Metab. Eng. (2013) In press
   PUBMED   24269999
REFERENCE   2  (bases 1 to 11697)
  AUTHORS   Kozak BU, van Rossum HM, Benjamin KR, Wu L, Daran J-M.G., Pronk JT,
            van Maris AJA.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAY-2013) Biotechnology, Delft University of
            Technology, Julianalaan 67, Delft 2628BC, The Netherlands
REFERENCE   3  (bases 1 to 11697)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11697)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: IDBA v. 0.20
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Metab. Eng.
            (2013) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-MAY-2013) Biotechnology, Delft University of Technology,
            Julianalaan 67, Delft 2628BC, The Netherlands"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11697
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(183..771)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(945..1802)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1803..1907)
                     /label="AmpR promoter"
     rep_origin      complement(1934..3276)
                     /direction=LEFT
                     /label="2u ori"
                     /note="yeast 2u plasmid origin of replication"
     promoter        3538..3753
                     /label="URA3 promoter"
     CDS             3754..4554
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     rep_origin      complement(4688..5143)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     5284..5300
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        5310..5328
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        5838..6357
                     /label="TPI1 promoter"
                     /note="strong constitutive promoter for yeast triose
                     phosphate isomerase"
     CDS             6358..8637
                     /gene="pflB"
                     /label="Formate acetyltransferase 1"
                     /note="Formate acetyltransferase 1 from Escherichia coli
                     (strain K12). Accession#: P09373"
     regulatory      8641..9141
                     /label="GDN2 terminator"
                     /note="GDN2 terminator"
                     /regulatory_class="terminator"
     regulatory      9146..10146
                     /label="FBA1 promoter"
                     /note="FBA1 promoter"
                     /regulatory_class="promoter"
     CDS             10147..10881
                     /gene="pflA"
                     /label="Pyruvate formate-lyase 1-activating enzyme"
                     /note="Pyruvate formate-lyase 1-activating enzyme from
                     Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 /
                     UPEC). Accession#: P0A9N5"
     regulatory      10885..11385
                     /label="PMA1 terminator"
                     /note="PMA1 terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(11386..11402)
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        complement(11439..11457)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11478..11494)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    11502..11518
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(11526..11556)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(11571..11592)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
ORIGIN
        1 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
       61 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
      121 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      181 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      241 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      301 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      361 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      421 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
      481 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
      541 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
      601 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
      661 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
      721 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
      781 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
      841 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
      901 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
      961 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     1021 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     1081 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     1141 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     1201 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     1261 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     1321 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     1381 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     1441 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     1501 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     1561 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     1621 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     1681 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     1741 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     1801 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     1861 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     1921 aaagtgccac ctgaacgaag catctgtgct tcattttgta gaacaaaaat gcaacgcgag
     1981 agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc
     2041 gaaagcgcta ttttaccaac gaagaatctg tgcttcattt ttgtaaaaca aaaatgcaac
     2101 gcgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc
     2161 aacgcgagag cgctatttta ccaacaaaga atctatactt cttttttgtt ctacaaaaat
     2221 gcatcccgag agcgctattt ttctaacaaa gcatcttaga ttactttttt tctcctttgt
     2281 gcgctctata atgcagtctc ttgataactt tttgcactgt aggtccgtta aggttagaag
     2341 aaggctactt tggtgtctat tttctcttcc ataaaaaaag cctgactcca cttcccgcgt
     2401 ttactgatta ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat ccccgattat
     2461 attctatacc gatgtggatt gcgcatactt tgtgaacaga aagtgatagc gttgatgatt
     2521 cttcattggt cagaaaatta tgaacggttt cttctatttt gtctctatat actacgtata
     2581 ggaaatgttt acattttcgt attgttttcg attcactcta tgaatagttc ttactacaat
     2641 ttttttgtct aaagagtaat actagagata aacataaaaa atgtagaggt cgagtttaga
     2701 tgcaagttca aggagcgaaa ggtggatggg taggttatat agggatatag cacagagata
     2761 tatagcaaag agatactttt gagcaatgtt tgtggaagcg gtattcgcaa tattttagta
     2821 gctcgttaca gtccggtgcg tttttggttt tttgaaagtg cgtcttcaga gcgcttttgg
     2881 ttttcaaaag cgctctgaag ttcctatact ttctagagaa taggaacttc ggaataggaa
     2941 cttcaaagcg tttccgaaaa cgagcgcttc cgaaaatgca acgcgagctg cgcacataca
     3001 gctcactgtt cacgtcgcac ctatatctgc gtgttgcctg tatatatata tacatgagaa
     3061 gaacggcata gtgcgtgttt atgcttaaat gcgtacttat atgcgtctat ttatgtagga
     3121 tgaaaggtag tctagtacct cctgtgatat tatcccattc catgcggggt atcgtatgct
     3181 tccttcagca ctacccttta gctgttctat atgctgccac tcctcaattg gattagtctc
     3241 atccttcaat gctatcattt cctttgatat tggatcatac taagaaacca ttattatcat
     3301 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
     3361 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     3421 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     3481 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata ccaccttttc
     3541 aattcatcat ttttttttta ttcttttttt tgatttcggt ttccttgaaa tttttttgat
     3601 tcggtaatct ccgaacagaa ggaagaacga aggaaggagc acagacttag attggtatat
     3661 atacgcatat gtagtgttga agaaacatga aattgcccag tattcttaac ccaactgcac
     3721 agaacaaaaa cctgcaggaa acgaagataa atcatgtcga aagctacata taaggaacgt
     3781 gctgctactc atcctagtcc tgttgctgcc aagctattta atatcatgca cgaaaagcaa
     3841 acaaacttgt gtgcttcatt ggatgttcgt accaccaagg aattactgga gttagttgaa
     3901 gcattaggtc ccaaaatttg tttactaaaa acacatgtgg atatcttgac tgatttttcc
     3961 atggagggca cagttaagcc gctaaaggca ttatccgcca agtacaattt tttactcttc
     4021 gaagacagaa aatttgctga cattggtaat acagtcaaat tgcagtactc tgcgggtgta
     4081 tacagaatag cagaatgggc agacattacg aatgcacacg gtgtggtggg cccaggtatt
     4141 gttagcggtt tgaagcaggc ggcagaagaa gtaacaaagg aacctagagg ccttttgatg
     4201 ttagcagaat tgtcatgcaa gggctcccta tctactggag aatatactaa gggtactgtt
     4261 gacattgcga agagcgacaa agattttgtt atcggcttta ttgctcaaag agacatgggt
     4321 ggaagagatg aaggttacga ttggttgatt atgacacccg gtgtgggttt agatgacaag
     4381 ggagacgcat tgggtcaaca gtatagaacc gtggatgatg tggtctctac aggatctgac
     4441 attattattg ttggaagagg actatttgca aagggaaggg atgctaaggt agagggtgaa
     4501 cgttacagaa aagcaggctg ggaagcatat ttgagaagat gcggccagca aaactaaaaa
     4561 actgtattat aagtaaatgc atgtatacta aactcacaaa ttagagcttc aatttaatta
     4621 tatcagttat taccctatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     4681 catcaggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     4741 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     4801 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     4861 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     4921 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     4981 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     5041 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     5101 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtccattcg ccattcaggc
     5161 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     5221 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     5281 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
     5341 ccgggccccc cctcgagaag gatgagccaa gaataaggga acaagatttt ggtaatttcc
     5401 aaaaaatcaa tagcatgcag gacgttatga agaagagatc tacgtatggt catttcttct
     5461 tcagattccc tcatggagaa agtgcggcag atgtatatga cagagtcgcc agtttccaag
     5521 agactttatt caggcacttc catgataggc aagagagaag acccagagat gttgttgtcc
     5581 tagttacaca tggtatttat tccagagtat tcctgatgaa atggtttaga tggacatacg
     5641 aagagtttga atcgtttacc aatgttccta acgggagcgt aatggtgatg gaactggacg
     5701 aatccatcaa tagatacgtc ctgaggaccg tgctacccaa atggactgat tgtgagggag
     5761 acctaactac atagtgttta aagattacgg atatttaact tacttagaat aatgccattt
     5821 ttttgagtta taataatcct acgttagtgt gagcgggatt taaactgtga ggaccttaat
     5881 acattcagac acttctgcgg tatcacccta cttattccct tcgagattat atctaggaac
     5941 ccatcaggtt ggtggaagat tacccgttct aagacttttc agcttcctct attgatgtta
     6001 cacctggaca ccccttttct ggcatccagt ttttaatctt cagtggcatg tgagattctc
     6061 cgaaattaat taaagcaatc acacaattct ctcggatacc acctcggttg aaactgacag
     6121 gtggtttgtt acgcatgcta atgcaaagga gcctatatac ctttggctcg gctgctgtaa
     6181 cagggaatat aaagggcagc ataatttagg agtttagtga acttgcaaca tttactattt
     6241 tcccttctta cgtaaatatt tttcttttta attctaaatc aatctttttc aattttttgt
     6301 ttgtattctt ttcttgctta aatctataac tacaaaaaac acatacataa actaaaaatg
     6361 tccgaattga atgaaaaatt ggctactgct tgggaaggtt tcaccaaggg tgactggcaa
     6421 aacgaagtca acgttagaga tttcatccaa aagaactaca ctccatacga aggtgatgaa
     6481 tctttcttgg ccggtgctac tgaagctacc accactttat gggacaaggt catggaaggt
     6541 gtcaaattgg aaaacagaac ccacgctcca gtcgatttcg acactgctgt tgcttccacc
     6601 atcacttccc acgatgctgg ttacatcaac aagcaattgg aaaagattgt tggtttgcaa
     6661 actgaagctc cattgaagag agctttgatt ccattcggtg gtatcaagat gattgaaggt
     6721 tcctgtaagg cttacaacag agaattggac ccaatgatca agaagatctt cactgaatac
     6781 agaaagactc acaaccaagg tgtctttgat gtttacactc cagacatctt gagatgtaga
     6841 aagtctggtg ttttgaccgg tttgccagat gcttacggta gaggtcgtat tattggtgac
     6901 tacagaagag ttgctctata cggtatcgat tatttgatga aggacaaatt ggctcaattc
     6961 acctctttgc aagctgattt ggaaaacggt gtcaacttgg aacaaaccat cagattacgt
     7021 gaagaaattg ctgaacaaca ccgtgctttg ggtcaaatga aggaaatggc tgccaagtac
     7081 ggttacgaca tctctggtcc agctaccaat gctcaagaag ccatccaatg gacctacttt
     7141 ggttacttgg ctgctgtcaa gtctcaaaac ggtgctgcta tgtctttcgg tagaacttct
     7201 actttcttgg atgtttacat tgaacgtgac ttgaaggccg gtaagatcac tgaacaagaa
     7261 gctcaagaaa tggttgacca tttagtcatg aagttgagaa tggttagatt tttgagaacc
     7321 ccagaatacg acgaattatt ctctggtgac ccaatctggg ctaccgaatc tatcggtggt
     7381 atgggtttgg acggtagaac cttggttacc aagaactctt tcagattctt gaacacttta
     7441 tacaccatgg gtccttcccc agaaccaaac atgaccatct tatggtccga aaagttgcca
     7501 ttaaacttca agaaattcgc tgccaaggtt tccattgaca cttcttcttt gcaatacgaa
     7561 aacgatgatt tgatgcgtcc agacttcaac aacgacgact acgccattgc ttgttgtgtt
     7621 tctccaatga ttgtcggtaa gcaaatgcaa ttcttcggtg ccagagctaa cttagctaag
     7681 accatgttgt acgccatcaa cggtggtgtc gatgaaaaat tgaagatgca agtcggtcca
     7741 aagtctgaac caatcaaggg tgacgttttg aactacgatg aagtcatgga aagaatggac
     7801 catttcatgg actggttagc taagcaatac atcactgctt tgaacatcat tcactacatg
     7861 cacgacaaat actcctacga agcttctttg atggctttgc acgacagaga tgtcatcaga
     7921 accatggctt gtggtattgc tggtctatct gttgctgctg actccttgtc tgccatcaaa
     7981 tacgccaagg tcaagcctat cagagatgaa gatggtttgg ccattgactt cgaaatcgaa
     8041 ggtgaatacc ctcaattcgg taacaacgat ccaagagttg acgatttggc tgttgacttg
     8101 gtcgaaagat tcatgaagaa gatccaaaaa ttgcacacct acagagatgc cattccaact
     8161 caatctgttt tgactatcac ttccaacgtt gtctacggta aaaagactgg taacactcca
     8221 gacggtagaa gagctggtgc cccattcggt ccaggtgcta acccaatgca cggtcgtgac
     8281 caaaagggtg ctgttgcctc tttgacctct gttgctaagc taccatttgc ttacgctaag
     8341 gacggtattt cttacacttt ctccattgtc ccaaatgctt tgggtaagga cgatgaagtt
     8401 agaaagacca acttggccgg tttgatggac ggttacttcc accacgaagc ttccattgaa
     8461 ggtggtcaac atttgaacgt caacgttatg aacagagaaa tgttgttgga cgctatggaa
     8521 aacccagaaa aatacccaca attgaccatc agagtttccg gttacgccgt tagattcaac
     8581 tccttgacca aggaacaaca acaagatgtc atcaccagaa ctttcactca atcaatgtaa
     8641 ggagttaaag gcaaagtttt cttttctaga gccgttccca caaataatta tacgtatatg
     8701 cttcttttcg tttactatat atctatattt acaagccttt attcactgat gcaatttgtt
     8761 tccaaatact tttttggaga tctcataact agatatgatg atggcgcaac ttgggcgtat
     8821 cttaattact ctggctgcca ggcccgtgta gagggccgca agaccttctg tacgccatat
     8881 agtctctaag aacttgaaca tgtttctaga cctattgccg cctttcggat cgctattgtt
     8941 catcatggat atttgccatc tcgtcttacc gacatcaaaa gggtgtgtgc atatagcagc
     9001 tatcatccca cttatgcaac cactggcaaa actgtttata aaatggaccc agtttgcgtc
     9061 cttagatgca aatcgagtag aatctagcca tagtctttcc ttgcaaagtt cataggaact
     9121 ccaatatatt gcactaaacg gaattcactg gtagagagcg actttgtatg ccccaattgc
     9181 gaaacccgcg ttatccttct cgattcttta gtacccgacc aggacaagga aaaggaggtc
     9241 gaaacgtttt tgaagaaaca agaggaacta cacggaagct ctaaagatgg caaccagcca
     9301 gaaactaaga aaatgaagtt gatggttcca actggcaccg ctggcttgaa caacaatacc
     9361 agccttccaa cttctgtaaa taacggcggt acgccagtgc caccagtacc gttacctttc
     9421 ggtatacctc ctttccccat gtttccaatg cccttcatgc ctccaacggc tactatcaca
     9481 aatcctcatc aagctgacgc aagccctaag aaatgaataa caatactgac agtactaaat
     9541 aattgcctac ttggcttcac atacgttgca tacgtcgata tagataataa tgataatgac
     9601 agcaggatta tcgtaatacg taatagttga aaatctcaaa aatgtgtggg tcattacgta
     9661 aataatgata ggaatgggat tcttctattt ttcctttttc cattctagca gccgtcggga
     9721 aaacgtggca tcctctcttt cgggctcaat tggagtcacg ctgccgtgag catcctctct
     9781 ttccatatct aacaactgag cacgtaacca atggaaaagc atgagcttag cgttgctcca
     9841 aaaaagtatt ggatggttaa taccatttgt ctgttctctt ctgactttga ctcctcaaaa
     9901 aaaaaaaatc tacaatcaac agatcgcttc aattacgccc tcacaaaaac ttttttcctt
     9961 cttcttcgcc cacgttaaat tttatccctc atgttgtcta acggatttct gcacttgatt
    10021 tattataaaa agacaaagac ataatacttc tctatcaatt tcagttattg ttcttccttg
    10081 cgttattctt ctgttcttct ttttcttttg tcatatataa ccataaccaa gtaatacata
    10141 ttcaaaatgt ccgtcattgg tcgtattcac tctttcgaat cttgtggtac tgtcgatggt
    10201 ccaggtatca gattcatcac tttcttccaa ggttgtttga tgagatgttt gtactgtcac
    10261 aacagagaca cctgggacac ccacggtggt aaggaagtca ctgttgaaga tttgatgaag
    10321 gaagttgtca cttacagaca tttcatgaac gcttctggtg gtggtgtcac tgcttctggt
    10381 ggtgaagcca tcttacaagc tgaatttgtt agagactggt tcagagcttg taagaaggaa
    10441 ggtattcaca cctgtttgga caccaacggt ttcgtcagaa gatacgaccc agttatcgat
    10501 gaattgttgg aagttaccga tttggtcatg ttggacttga aacaaatgaa cgatgaaatt
    10561 caccaaaact tggttggtgt ttccaaccac agaactttgg aatttgccaa gtacttggct
    10621 aacaagaacg tcaaggtctg gatcagatac gttgttgttc caggttggtc cgatgacgat
    10681 gactctgctc acagattagg tgaatttacc agagacatgg gtaacgtcga aaagatcgaa
    10741 ttgctaccat accatgaatt aggtaagcac aaatgggttg ctatgggtga agaatacaaa
    10801 ttggacggtg ttaagccacc aaagaaggaa accatggaac gtgtcaaggg tatcttggaa
    10861 caatacggtc acaaggttat gtaagctcct gttgaagtag catttaatca taatttttgt
    10921 cacattttaa tcaacttgat ttttctggtt taatttttct aattttaatt ttaatttttt
    10981 tatcaatggg aactgataca ctaaaaagaa ttaggagcca acaagaataa gccgcttatt
    11041 tcctactaga gtttgcttaa aatttcatct cgaattgtca ttctaatatt ttatccacac
    11101 acacacctta aaatttttag attaaatggc atcaactctt agcttcacac acacacacac
    11161 accgaagctg gttgttttat ttgatttgat ataattggtt tctctggatg gtactttttc
    11221 tttcttggtt atttcctatt ttaaaatatg aaacgcacac aagtcataat tattctaata
    11281 gagcacaatt cacaacacgc acatttcaac tttaatattt ttttagaaac actttattta
    11341 gtctaattct taatttttaa tatatataat gcacacacac gtttggatcc actagttcta
    11401 gagcggccgc caccgcggtg gagctccagc ttttgttccc tttagtgagg gttaattgcg
    11461 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
    11521 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
    11581 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
    11641 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgc
//
