LOCUS       40924_45448       11861 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pUDE202, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11861)
  AUTHORS   Kozak BU, van Rossum HM, Benjamin KR, Wu L, Daran JM, Pronk JT, van 
            Maris AJ.
  TITLE     Replacement of the Saccharomyces cerevisiae acetyl-CoA synthetases 
            by alternative pathways for cytosolic acetyl-CoA synthesis
  JOURNAL   Metab. Eng. (2013) In press
  PUBMED    24269999
REFERENCE   2  (bases 1 to 11861)
  AUTHORS   Kozak BU, van Rossum HM, Benjamin KR, Wu L, Daran J-M.G., Pronk JT, 
            van Maris AJA.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAY-2013) Biotechnology, Delft University of 
            Technology, Julianalaan 67, Delft 2628BC, The Netherlands
REFERENCE   3  (bases 1 to 11861)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11861)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab. Eng.
            (2013) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-MAY-2013) Biotechnology, Delft University of Technology, 
            Julianalaan 67, Delft 2628BC, The Netherlands"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: IDBA v. 0.20
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(233..821)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(995..1852)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(1853..1957)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1984..3326)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        3588..3803
                     /label=URA3 promoter
     CDS             3804..4604
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     rep_origin      complement(4738..5193)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     5334..5350
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5360..5378
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        5888..6407
                     /label=TPI1 promoter
                     /note="strong constitutive promoter for yeast triose
                     phosphate isomerase"
     CDS             6408..8720
                     /codon_start=1
                     /gene="pfl"
                     /product="pyruvate formate lyase"
                     /label=pfl
                     /protein_id="AHB33308.1"
                     /translation="MAAASAAAEALPVAPSHSCAADPDKHPHLPDPRPKPAVDAGINVQ
                     KYVQDNYTAYAGNSSFLAGPTDNTKKLWSELEKMIATEIEKGVMDVDPSKPSTITAFPP
                     GYIDKDLETVVGLQTDAPLKRAIKPLGGVNMVKAALESYGYTPDPEVARLYSTVRKTHN
                     SGVFDAYTDEMRAARKSGILSGLPDGYGRGRIIGDYRRVALYGVDALIKAKKTDLKHNL
                     LGVMDEEKIRLREEVNEQIRALSELKEMGAAYGFDLSRPAANSREAVQWLYFGYLGAVK
                     EQDGAAMSLGRIDAFLDTYFERDLKAGTITEAEVQELIDHFVMKLRIVRQLRTPEYNAL
                     FAGDPTWVTCVLGGTDASGKAMVTKTSFRLLNTLYNLGPAPEPNLTVLWNDNLPAPFKE
                     FCAKVSLDTSSIQYESDNLMSKLFGSDYSIACCVSAMRVGKDMQYFGARANLPKLLLYT
                     LNGGRDEVSGDQVGPKFAPVRSPTAPLDYEEVKAKIEDGMEWLASMYANTMNIIHYMHD
                     KYDYERLQMALHDTHVRRLLAFGISGLSVVTDSLSAIEYAQVTPVIDERGLMTDFKVEG
                     SFPKYGNDDDRVDEIAEWVVSTFSSKLAKQHTYRNSVPTLSVLTITSNVVYGKKTGSTP
                     DGRKKGEPIAPGDNPLHGRDAHGALASLNSVAKLPYTMCLDGISNTFSLIPQVLGRGGE
                     HERATNLASILDGYFANGGHHINVNVLNRSMLMDAVEHPEKYPNLTIRVSGYAVHFARL
                     TREQQLEVIARTFHDTM"
     gene            6408..8720
                     /gene="pfl"
                     /label=pfl
     regulatory      8671..9171
                     /label=GND2 terminator
                     /note="GND2 terminator"
                     /regulatory_class="terminator"
     regulatory      9176..10176
                     /label=FBA1 promoter
                     /note="FBA1 promoter"
                     /regulatory_class="promoter"
     CDS             10227..11048
                     /codon_start=1
                     /gene="pflA"
                     /product="PFL activating enzyme"
                     /label=pflA
                     /protein_id="AHB33309.1"
                     /translation="MERLQPKLSTNYSVVLPQYEPTDPSGIPEVFGNVHSTESMSAVDG
                     PGVRFLVFVQGCAMRCLFCSNPDTWTLKGGNKTSSKEIAADIKRVRNYLKPRGGITISG
                     GEAMLQPHFVSTVFQEVHALGLNTTVDTTGQGTKAGNWDVVLPHTDLVLFCIKHIDPIK
                     YEALTGMKQRGALRFADQLAERKIPFYLRYVYIPGYTDGVKDIDKLIEWCKQQPTFQGI
                     ELLPYHVLGRNKWEVMGLPYPLDGTNTPPHEQVRAVIKVFNDNDVPVICAE"
     gene            10227..11048
                     /gene="pflA"
                     /label=pflA
     regulatory      10999..11499
                     /label=PMA1 terminator
                     /note="PMA1 terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(11550..11566)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(11603..11621)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11642..11658)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    11666..11682
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(11690..11720)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(11735..11756)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
ORIGIN
        1 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
       61 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
      121 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      181 tttttccata ggctccgccc aggaaccgta aaaaggccgc gttgctggcg tttttccata
      241 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
      301 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
      361 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
      421 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
      481 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
      541 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
      601 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
      661 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
      721 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
      781 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
      841 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
      901 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
      961 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     1021 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     1081 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     1141 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     1201 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     1261 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     1321 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     1381 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     1441 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     1501 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     1561 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     1621 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     1681 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     1741 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     1801 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     1861 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     1921 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     1981 ctgaacgaag catctgtgct tcattttgta gaacaaaaat gcaacgcgag agcgctaatt
     2041 tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc gaaagcgcta
     2101 ttttaccaac gaagaatctg tgcttcattt ttgtaaaaca aaaatgcaac gcgagagcgc
     2161 taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgagag
     2221 cgctatttta ccaacaaaga atctatactt cttttttgtt ctacaaaaat gcatcccgag
     2281 agcgctattt ttctaacaaa gcatcttaga ttactttttt tctcctttgt gcgctctata
     2341 atgcagtctc ttgataactt tttgcactgt aggtccgtta aggttagaag aaggctactt
     2401 tggtgtctat tttctcttcc ataaaaaaag cctgactcca cttcccgcgt ttactgatta
     2461 ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat ccccgattat attctatacc
     2521 gatgtggatt gcgcatactt tgtgaacaga aagtgatagc gttgatgatt cttcattggt
     2581 cagaaaatta tgaacggttt cttctatttt gtctctatat actacgtata ggaaatgttt
     2641 acattttcgt attgttttcg attcactcta tgaatagttc ttactacaat ttttttgtct
     2701 aaagagtaat actagagata aacataaaaa atgtagaggt cgagtttaga tgcaagttca
     2761 aggagcgaaa ggtggatggg taggttatat agggatatag cacagagata tatagcaaag
     2821 agatactttt gagcaatgtt tgtggaagcg gtattcgcaa tattttagta gctcgttaca
     2881 gtccggtgcg tttttggttt tttgaaagtg cgtcttcaga gcgcttttgg ttttcaaaag
     2941 cgctctgaag ttcctatact ttctagagaa taggaacttc ggaataggaa cttcaaagcg
     3001 tttccgaaaa cgagcgcttc cgaaaatgca acgcgagctg cgcacataca gctcactgtt
     3061 cacgtcgcac ctatatctgc gtgttgcctg tatatatata tacatgagaa gaacggcata
     3121 gtgcgtgttt atgcttaaat gcgtacttat atgcgtctat ttatgtagga tgaaaggtag
     3181 tctagtacct cctgtgatat tatcccattc catgcggggt atcgtatgct tccttcagca
     3241 ctacccttta gctgttctat atgctgccac tcctcaattg gattagtctc atccttcaat
     3301 gctatcattt cctttgatat tggatcatac taagaaacca ttattatcat gacattaacc
     3361 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     3421 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     3481 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     3541 tatgcggcat cagagcagat tgtactgaga gtgcaccata ccaccttttc aattcatcat
     3601 ttttttttta ttcttttttt tgatttcggt ttccttgaaa tttttttgat tcggtaatct
     3661 ccgaacagaa ggaagaacga aggaaggagc acagacttag attggtatat atacgcatat
     3721 gtagtgttga agaaacatga aattgcccag tattcttaac ccaactgcac agaacaaaaa
     3781 cctgcaggaa acgaagataa atcatgtcga aagctacata taaggaacgt gctgctactc
     3841 atcctagtcc tgttgctgcc aagctattta atatcatgca cgaaaagcaa acaaacttgt
     3901 gtgcttcatt ggatgttcgt accaccaagg aattactgga gttagttgaa gcattaggtc
     3961 ccaaaatttg tttactaaaa acacatgtgg atatcttgac tgatttttcc atggagggca
     4021 cagttaagcc gctaaaggca ttatccgcca agtacaattt tttactcttc gaagacagaa
     4081 aatttgctga cattggtaat acagtcaaat tgcagtactc tgcgggtgta tacagaatag
     4141 cagaatgggc agacattacg aatgcacacg gtgtggtggg cccaggtatt gttagcggtt
     4201 tgaagcaggc ggcagaagaa gtaacaaagg aacctagagg ccttttgatg ttagcagaat
     4261 tgtcatgcaa gggctcccta tctactggag aatatactaa gggtactgtt gacattgcga
     4321 agagcgacaa agattttgtt atcggcttta ttgctcaaag agacatgggt ggaagagatg
     4381 aaggttacga ttggttgatt atgacacccg gtgtgggttt agatgacaag ggagacgcat
     4441 tgggtcaaca gtatagaacc gtggatgatg tggtctctac aggatctgac attattattg
     4501 ttggaagagg actatttgca aagggaaggg atgctaaggt agagggtgaa cgttacagaa
     4561 aagcaggctg ggaagcatat ttgagaagat gcggccagca aaactaaaaa actgtattat
     4621 aagtaaatgc atgtatacta aactcacaaa ttagagcttc aatttaatta tatcagttat
     4681 taccctatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggaaa
     4741 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     4801 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     4861 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     4921 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     4981 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     5041 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     5101 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     5161 ccgccgcgct taatgcgccg ctacagggcg cgtccattcg ccattcaggc tgcgcaactg
     5221 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     5281 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
     5341 gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta ccgggccccc
     5401 cctcgagaag gatgagccaa gaataaggga acaagatttt ggtaatttcc aaaaaatcaa
     5461 tagcatgcag gacgttatga agaagagatc tacgtatggt catttcttct tcagattccc
     5521 tcatggagaa agtgcggcag atgtatatga cagagtcgcc agtttccaag agactttatt
     5581 caggcacttc catgataggc aagagagaag acccagagat gttgttgtcc tagttacaca
     5641 tggtatttat tccagagtat tcctgatgaa atggtttaga tggacatacg aagagtttga
     5701 atcgtttacc aatgttccta acgggagcgt aatggtgatg gaactggacg aatccatcaa
     5761 tagatacgtc ctgaggaccg tgctacccaa atggactgat tgtgagggag acctaactac
     5821 atagtgttta aagattacgg atatttaact tacttagaat aatgccattt ttttgagtta
     5881 taataatcct acgttagtgt gagcgggatt taaactgtga ggaccttaat acattcagac
     5941 acttctgcgg tatcacccta cttattccct tcgagattat atctaggaac ccatcaggtt
     6001 ggtggaagat tacccgttct aagacttttc agcttcctct attgatgtta cacctggaca
     6061 ccccttttct ggcatccagt ttttaatctt cagtggcatg tgagattctc cgaaattaat
     6121 taaagcaatc acacaattct ctcggatacc acctcggttg aaactgacag gtggtttgtt
     6181 acgcatgcta atgcaaagga gcctatatac ctttggctcg gctgctgtaa cagggaatat
     6241 aaagggcagc ataatttagg agtttagtga acttgcaaca tttactattt tcccttctta
     6301 cgtaaatatt tttcttttta attctaaatc aatctttttc aattttttgt ttgtattctt
     6361 ttcttgctta aatctataac tacaaaaaac acatacataa actaaaaatg gctgctgctt
     6421 ctgctgccgc tgaagcttta ccagttgctc catctcactc ttgtgctgct gacccagaca
     6481 agcatcctca tttgccagac ccaagaccaa agccagccgt cgatgctggt atcaacgttc
     6541 aaaaatacgt tcaagacaac tacactgctt acgctggtaa ctcttctttc ttggctggtc
     6601 caactgacaa caccaagaaa ttgtggtctg aattggaaaa gatgattgct actgaaatcg
     6661 aaaagggtgt catggatgtt gacccttcca agccatccac catcaccgct ttcccaccag
     6721 gttacatcga caaagatttg gaaaccgttg tcggtctaca aaccgatgct ccattaaaga
     6781 gagccatcaa gccattaggt ggtgtcaaca tggtcaaggc cgctttggaa tcttacggtt
     6841 acactccaga cccagaagtt gccagattgt actccaccgt tagaaagacc cacaactctg
     6901 gtgttttcga tgcttacact gacgaaatga gagctgccag aaagtctggt atcttatctg
     6961 gtttgccaga cggttacggt cgtggtagaa tcattggtga ctacagaaga gttgctttgt
     7021 acggtgttga cgctttgatc aaggccaaaa agaccgattt gaagcacaac ttgttgggtg
     7081 tcatggacga agaaaagatc agattaagag aagaagtcaa tgaacaaatc cgtgctttgt
     7141 ctgaattgaa ggaaatgggt gctgcttacg gtttcgattt gtccagacct gctgccaact
     7201 ccagagaagc cgtccaatgg ttatacttcg gttacttggg tgccgtcaag gaacaagatg
     7261 gtgctgctat gtctttgggt cgtatcgatg ctttcttgga tacctacttc gaaagagatt
     7321 tgaaggccgg tactattact gaagctgaag ttcaagaatt gattgaccat ttcgtcatga
     7381 aattgagaat cgttcgtcaa ttgagaactc cagaatacaa cgctttgttt gctggtgacc
     7441 caacctgggt tacttgtgtt ttgggtggta ctgatgcttc tggtaaggct atggttacca
     7501 agacctcttt cagattattg aacactttgt acaacttggg tccagctcca gaaccaaact
     7561 tgactgtctt atggaacgac aacttaccag ctccattcaa ggaattttgt gccaaggttt
     7621 ctttggacac ctcttccatc caatacgaat ctgacaactt gatgtccaag ttgttcggtt
     7681 ctgactactc catcgcttgt tgtgtttctg ccatgagagt cggtaaggac atgcaatatt
     7741 tcggtgctcg tgccaacttg ccaaagctat tattgtacac tttgaacggt ggtagagatg
     7801 aagtttccgg tgaccaagtc ggtccaaagt tcgctccagt cagatctcct accgctccat
     7861 tagactacga agaagttaag gccaagatcg aagatggtat ggaatggttg gcttccatgt
     7921 acgctaacac catgaacatt attcactaca tgcacgacaa atacgactac gaaagattac
     7981 aaatggcttt gcacgatacc cacgtcagaa gattgttggc tttcggtatt tctggtctat
     8041 ccgttgtcac tgattctttg tctgccattg aatacgctca agtcactcca gttatcgacg
     8101 aaagaggttt gatgactgac ttcaaggttg aaggttcttt cccaaagtac ggtaacgatg
     8161 acgacagagt cgatgaaatt gctgaatggg ttgtctccac tttctcctcc aaattggcca
     8221 agcaacacac ttacagaaac tctgttccaa ctttgtctgt tttgaccatc acttccaacg
     8281 ttgtctacgg taaaaagact ggttctaccc cagatggtag aaagaagggt gaaccaattg
     8341 ctccaggtga caacccattg cacggtagag atgctcacgg tgctttggct tctttgaact
     8401 ccgttgccaa gttgccatac accatgtgtt tggatggtat ctctaacact ttctccttga
     8461 ttccacaagt tttgggtcgt ggtggtgaac acgaaagagc taccaacttg gcttccatct
     8521 tggacggtta ctttgctaac ggtggtcacc acattaatgt caatgtcttg aacagatcca
     8581 tgttgatgga cgctgttgaa cacccagaaa agtacccaaa cttgaccatc agagtttccg
     8641 gttacgctgt ccacttcgct agattgacca gagaacaaca attggaagtc attgccagaa
     8701 ctttccacga caccatgtaa ggagttaaag gcaaagtttt cttttctaga gccgttccca
     8761 caaataatta tacgtatatg cttcttttcg tttactatat atctatattt acaagccttt
     8821 attcactgat gcaatttgtt tccaaatact tttttggaga tctcataact agatatgatg
     8881 atggcgcaac ttgggcgtat cttaattact ctggctgcca ggcccgtgta gagggccgca
     8941 agaccttctg tacgccatat agtctctaag aacttgaaca tgtttctaga cctattgccg
     9001 cctttcggat cgctattgtt catcatggat atttgccatc tcgtcttacc gacatcaaaa
     9061 gggtgtgtgc atatagcagc tatcatccca cttatgcaac cactggcaaa actgtttata
     9121 aaatggaccc agtttgcgtc cttagatgca aatcgagtag aatctagcca tagtctttcc
     9181 ttgcaaagtt cataggaact ccaatatatt gcactaaacg gaattcactg gtagagagcg
     9241 actttgtatg ccccaattgc gaaacccgcg ttatccttct cgattcttta gtacccgacc
     9301 aggacaagga aaaggaggtc gaaacgtttt tgaagaaaca agaggaacta cacggaagct
     9361 ctaaagatgg caaccagcca gaaactaaga aaatgaagtt gatggttcca actggcaccg
     9421 ctggcttgaa caacaatacc agccttccaa cttctgtaaa taacggcggt acgccagtgc
     9481 caccagtacc gttacctttc ggtatacctc ctttccccat gtttccaatg cccttcatgc
     9541 ctccaacggc tactatcaca aatcctcatc aagctgacgc aagccctaag aaatgaataa
     9601 caatactgac agtactaaat aattgcctac ttggcttcac atacgttgca tacgtcgata
     9661 tagataataa tgataatgac agcaggatta tcgtaatacg taatagttga aaatctcaaa
     9721 aatgtgtggg tcattacgta aataatgata ggaatgggat tcttctattt ttcctttttc
     9781 cattctagca gccgtcggga aaacgtggca tcctctcttt cgggctcaat tggagtcacg
     9841 ctgccgtgag catcctctct ttccatatct aacaactgag cacgtaacca atggaaaagc
     9901 atgagcttag cgttgctcca aaaaagtatt ggatggttaa taccatttgt ctgttctctt
     9961 ctgactttga ctcctcaaaa aaaaaaaatc tacaatcaac agatcgcttc aattacgccc
    10021 tcacaaaaac ttttttcctt cttcttcgcc cacgttaaat tttatccctc atgttgtcta
    10081 acggatttct gcacttgatt tattataaaa agacaaagac ataatacttc tctatcaatt
    10141 tcagttattg ttcttccttg cgttattctt ctgttcttct ttttcttttg tcatatataa
    10201 ccataaccaa gtaatacata ttcaaaatgg aaagattaca accaaagttg tctaccaact
    10261 actctgttgt cttaccacaa tacgaaccaa ctgacccatc tggtatccca gaagtctttg
    10321 gtaacgttca ctctactgaa tccatgtctg ccgtcgatgg tccaggtgtt agattcttgg
    10381 ttttcgttca aggttgtgcc atgagatgtt tgttctgttc caacccagac acctggactt
    10441 tgaagggtgg taacaagacc tcttccaagg aaattgctgc tgacatcaag cgtgtcagaa
    10501 actacttgaa gccaagaggt ggtattacca tctctggtgg tgaagctatg ttgcaacctc
    10561 acttcgtttc cactgttttc caagaagtcc acgctttggg tttgaacacc accgttgaca
    10621 ccaccggtca aggtactaag gctggtaact gggatgttgt cttaccacac accgatttgg
    10681 tcttattctg tatcaagcac attgacccaa tcaaatacga agctttgact ggtatgaagc
    10741 aaagaggtgc tttgagattt gctgaccaat tggctgaaag aaagatccca ttctacttgc
    10801 gttacgtcta cattccaggt tacaccgatg gtgtcaagga tattgacaaa ttgattgaat
    10861 ggtgtaagca acaaccaact ttccaaggta tcgaattgtt gccataccat gttttgggta
    10921 gaaacaaatg ggaagtcatg ggtctaccat acccattgga cggtactaac actccacctc
    10981 acgaacaagt cagagctgtt atcaaggttt tcaacgacaa cgatgttcca gttatctgtg
    11041 ccgagtaagc tcctgttgaa gtagcattta atcataattt ttgtcacatt ttaatcaact
    11101 tgatttttct ggtttaattt ttctaatttt aattttaatt tttttatcaa tgggaactga
    11161 tacactaaaa agaattagga gccaacaaga ataagccgct tatttcctac tagagtttgc
    11221 ttaaaatttc atctcgaatt gtcattctaa tattttatcc acacacacac cttaaaattt
    11281 ttagattaaa tggcatcaac tcttagcttc acacacacac acacaccgaa gctggttgtt
    11341 ttatttgatt tgatataatt ggtttctctg gatggtactt tttctttctt ggttatttcc
    11401 tattttaaaa tatgaaacgc acacaagtca taattattct aatagagcac aattcacaac
    11461 acgcacattt caactttaat atttttttag aaacacttta tttagtctaa ttcttaattt
    11521 ttaatatata taatgcacac acacgtttgg atccactagt tctagagcgg ccgccaccgc
    11581 ggtggagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat
    11641 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
    11701 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
    11761 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
    11821 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg c
//
