LOCUS       40924_45443       11595 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pUDE201, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11595)
  AUTHORS   Kozak BU, van Rossum HM, Benjamin KR, Wu L, Daran JM, Pronk JT, van 
            Maris AJ.
  TITLE     Replacement of the Saccharomyces cerevisiae acetyl-CoA synthetases 
            by alternative pathways for cytosolic acetyl-CoA synthesis
  JOURNAL   Metab. Eng. (2013) In press
  PUBMED    24269999
REFERENCE   2  (bases 1 to 11595)
  AUTHORS   Kozak BU, van Rossum HM, Benjamin KR, Wu L, Daran J-M.G., Pronk JT, 
            van Maris AJA.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAY-2013) Biotechnology, Delft University of 
            Technology, Julianalaan 67, Delft 2628BC, The Netherlands
REFERENCE   3  (bases 1 to 11595)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11595)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab. Eng.
            (2013) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-MAY-2013) Biotechnology, Delft University of Technology, 
            Julianalaan 67, Delft 2628BC, The Netherlands"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: IDBA v. 0.20
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11595
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(183..771)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(945..1802)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(1803..1907)
                     /label=AmpR promoter
     rep_origin      complement(1934..3276)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        3538..3753
                     /label=URA3 promoter
     CDS             3754..4554
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     rep_origin      complement(4688..5143)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     5284..5300
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5310..5328
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        5838..6357
                     /label=TPI1 promoter
                     /note="strong constitutive promoter for yeast triose
                     phosphate isomerase"
     CDS             6358..8577
                     /codon_start=1
                     /gene="PFL"
                     /product="hypothetical protein"
                     /label=PFL
                     /protein_id="AHB33304.1"
                     /translation="MPGDCDVKSFINRYTTLYDGDASFLAGPTQRTQKALQKVKTLLAL
                     EREKGGVLSVDADIPSTITSHAPGYLLSKEEDVIVGLQAEEPLKRTCKPHGGFGVVKKA
                     LESYGYEPGEKLKVFADEVTTHNDLTFSIYTDKMKQARHAHLLTGLPDGYGRGRIIADY
                     RRIALYGVDALIQLKQADFAAVTGSSVEAMRLRSEIFHQIKALKELLVMADSYGVDLRQ
                     PASTFKDAAQALWLGHTAALKEQDGAAMSAGRWDSFLDIYAEKDLAAGVATEEDIQEVV
                     DDMVLKMRLVRHVRTPEYNELFTGDPTWTTLALGGATEDSKSMVTKTTYRFLHSLRNLG
                     PAPEPNLTVLWSKVHSSHFKEYCAQLSIDTSSIQYENDDLMRPIFGSDYAIACCVSAMR
                     IGTDMQFFGARTNLAKLLLMVLNGGRDELEGQLLSEPLAEACREAGIGAGDEDTPLDYE
                     KVSKLYFDVAIPWMAELYADTMNCIHYSHDATSYENLQMALHNSNVNHFMAFGIAGLSV
                     VADSLAAIKYDDVYPIRNENGLTVGFKRRHPESVLPTFGNDNPKVDDIAVEVADAFYEE
                     LNKQKLYKDAKATVSVLTITSNVVYGKSTGTTPDGRIIGEPFAPGANPMHNRDRNGAIA
                     SLASVAKLPYASCMDGISNTFCLTPTALGSMADVRAMNLVSLLDGYFGKNGHHLNINVL
                     NRELLEDAHLHPEKYPDLTIRVSGYAVRFNQLTPEQREEVLKRTM"
     gene            6358..8577
                     /gene="PFL"
                     /label=PFL
     regulatory      8578..9078
                     /label=GND2 terminator
                     /note="GND2 terminator"
                     /regulatory_class="terminator"
     regulatory      9083..10083
                     /label=FBA1 promoter
                     /note="FBA1 promoter"
                     /regulatory_class="promoter"
     CDS             10084..10782
                     /codon_start=1
                     /gene="PFL-AE"
                     /product="hypothetical protein"
                     /label=PFL-AE
                     /protein_id="AHB33305.1"
                     /translation="MIIGSVHSLETFTSNDGPGIRTLVFLQGCSKRCMYCSNPETQCIV
                     DPFSCPEVAVSDEEVANVLKRYEHFLRPNNGGITFSGGEPLLQPNFVGSVFKKAKSIGL
                     TTCVDTSGHGNPAIWNKCLPHTDYVMLCLKGMDMKLASFISGVSESNNQRAREFAKYVR
                     DNYKNIKLSIRWVLLKDMTDTDEELDALAAFTKDLSPVFTHVELLPYHILGKDKYEAMN
                     SLYPMEGMEP"
     gene            10084..10782
                     /gene="PFL-AE"
                     /label=PFL-AE
     regulatory      10783..11283
                     /label=PMA1 terminator
                     /note="PMA1 terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(11284..11300)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(11337..11355)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11376..11392)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    11400..11416
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(11424..11454)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(11469..11490)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
ORIGIN
        1 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
       61 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
      121 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      181 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      241 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      301 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      361 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      421 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
      481 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
      541 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
      601 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
      661 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
      721 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
      781 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
      841 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
      901 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
      961 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     1021 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     1081 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     1141 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     1201 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     1261 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     1321 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     1381 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     1441 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     1501 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     1561 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     1621 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     1681 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     1741 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     1801 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     1861 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     1921 aaagtgccac ctgaacgaag catctgtgct tcattttgta gaacaaaaat gcaacgcgag
     1981 agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc
     2041 gaaagcgcta ttttaccaac gaagaatctg tgcttcattt ttgtaaaaca aaaatgcaac
     2101 gcgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc
     2161 aacgcgagag cgctatttta ccaacaaaga atctatactt cttttttgtt ctacaaaaat
     2221 gcatcccgag agcgctattt ttctaacaaa gcatcttaga ttactttttt tctcctttgt
     2281 gcgctctata atgcagtctc ttgataactt tttgcactgt aggtccgtta aggttagaag
     2341 aaggctactt tggtgtctat tttctcttcc ataaaaaaag cctgactcca cttcccgcgt
     2401 ttactgatta ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat ccccgattat
     2461 attctatacc gatgtggatt gcgcatactt tgtgaacaga aagtgatagc gttgatgatt
     2521 cttcattggt cagaaaatta tgaacggttt cttctatttt gtctctatat actacgtata
     2581 ggaaatgttt acattttcgt attgttttcg attcactcta tgaatagttc ttactacaat
     2641 ttttttgtct aaagagtaat actagagata aacataaaaa atgtagaggt cgagtttaga
     2701 tgcaagttca aggagcgaaa ggtggatggg taggttatat agggatatag cacagagata
     2761 tatagcaaag agatactttt gagcaatgtt tgtggaagcg gtattcgcaa tattttagta
     2821 gctcgttaca gtccggtgcg tttttggttt tttgaaagtg cgtcttcaga gcgcttttgg
     2881 ttttcaaaag cgctctgaag ttcctatact ttctagagaa taggaacttc ggaataggaa
     2941 cttcaaagcg tttccgaaaa cgagcgcttc cgaaaatgca acgcgagctg cgcacataca
     3001 gctcactgtt cacgtcgcac ctatatctgc gtgttgcctg tatatatata tacatgagaa
     3061 gaacggcata gtgcgtgttt atgcttaaat gcgtacttat atgcgtctat ttatgtagga
     3121 tgaaaggtag tctagtacct cctgtgatat tatcccattc catgcggggt atcgtatgct
     3181 tccttcagca ctacccttta gctgttctat atgctgccac tcctcaattg gattagtctc
     3241 atccttcaat gctatcattt cctttgatat tggatcatac taagaaacca ttattatcat
     3301 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
     3361 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     3421 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     3481 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata ccaccttttc
     3541 aattcatcat ttttttttta ttcttttttt tgatttcggt ttccttgaaa tttttttgat
     3601 tcggtaatct ccgaacagaa ggaagaacga aggaaggagc acagacttag attggtatat
     3661 atacgcatat gtagtgttga agaaacatga aattgcccag tattcttaac ccaactgcac
     3721 agaacaaaaa cctgcaggaa acgaagataa atcatgtcga aagctacata taaggaacgt
     3781 gctgctactc atcctagtcc tgttgctgcc aagctattta atatcatgca cgaaaagcaa
     3841 acaaacttgt gtgcttcatt ggatgttcgt accaccaagg aattactgga gttagttgaa
     3901 gcattaggtc ccaaaatttg tttactaaaa acacatgtgg atatcttgac tgatttttcc
     3961 atggagggca cagttaagcc gctaaaggca ttatccgcca agtacaattt tttactcttc
     4021 gaagacagaa aatttgctga cattggtaat acagtcaaat tgcagtactc tgcgggtgta
     4081 tacagaatag cagaatgggc agacattacg aatgcacacg gtgtggtggg cccaggtatt
     4141 gttagcggtt tgaagcaggc ggcagaagaa gtaacaaagg aacctagagg ccttttgatg
     4201 ttagcagaat tgtcatgcaa gggctcccta tctactggag aatatactaa gggtactgtt
     4261 gacattgcga agagcgacaa agattttgtt atcggcttta ttgctcaaag agacatgggt
     4321 ggaagagatg aaggttacga ttggttgatt atgacacccg gtgtgggttt agatgacaag
     4381 ggagacgcat tgggtcaaca gtatagaacc gtggatgatg tggtctctac aggatctgac
     4441 attattattg ttggaagagg actatttgca aagggaaggg atgctaaggt agagggtgaa
     4501 cgttacagaa aagcaggctg ggaagcatat ttgagaagat gcggccagca aaactaaaaa
     4561 actgtattat aagtaaatgc atgtatacta aactcacaaa ttagagcttc aatttaatta
     4621 tatcagttat taccctatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     4681 catcaggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     4741 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     4801 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     4861 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     4921 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     4981 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     5041 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     5101 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtccattcg ccattcaggc
     5161 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     5221 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     5281 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
     5341 ccgggccccc cctcgagaag gatgagccaa gaataaggga acaagatttt ggtaatttcc
     5401 aaaaaatcaa tagcatgcag gacgttatga agaagagatc tacgtatggt catttcttct
     5461 tcagattccc tcatggagaa agtgcggcag atgtatatga cagagtcgcc agtttccaag
     5521 agactttatt caggcacttc catgataggc aagagagaag acccagagat gttgttgtcc
     5581 tagttacaca tggtatttat tccagagtat tcctgatgaa atggtttaga tggacatacg
     5641 aagagtttga atcgtttacc aatgttccta acgggagcgt aatggtgatg gaactggacg
     5701 aatccatcaa tagatacgtc ctgaggaccg tgctacccaa atggactgat tgtgagggag
     5761 acctaactac atagtgttta aagattacgg atatttaact tacttagaat aatgccattt
     5821 ttttgagtta taataatcct acgttagtgt gagcgggatt taaactgtga ggaccttaat
     5881 acattcagac acttctgcgg tatcacccta cttattccct tcgagattat atctaggaac
     5941 ccatcaggtt ggtggaagat tacccgttct aagacttttc agcttcctct attgatgtta
     6001 cacctggaca ccccttttct ggcatccagt ttttaatctt cagtggcatg tgagattctc
     6061 cgaaattaat taaagcaatc acacaattct ctcggatacc acctcggttg aaactgacag
     6121 gtggtttgtt acgcatgcta atgcaaagga gcctatatac ctttggctcg gctgctgtaa
     6181 cagggaatat aaagggcagc ataatttagg agtttagtga acttgcaaca tttactattt
     6241 tcccttctta cgtaaatatt tttcttttta attctaaatc aatctttttc aattttttgt
     6301 ttgtattctt ttcttgctta aatctataac tacaaaaaac acatacataa actaaaaatg
     6361 ccaggtgact gtgatgtcaa atctttcatc aacagataca ctactttata cgatggtgac
     6421 gcttctttct tggccggtcc aactcaaaga acccaaaagg ctttgcaaaa ggttaagact
     6481 ttgttggctt tggaaagaga aaagggtggt gttttgtccg tcgatgctga tattccatct
     6541 accatcacct ctcacgctcc aggttacttg ttgtccaagg aagaagatgt cattgttggt
     6601 ttgcaagctg aagaaccttt gaagagaacc tgtaagcctc acggtggttt cggtgttgtc
     6661 aagaaggctt tggaatctta cggttacgaa ccaggtgaaa agttgaaggt tttcgctgac
     6721 gaagtcacca cccacaacga cttgactttc tccatctaca ctgacaagat gaagcaagct
     6781 agacatgccc acttgttgac tggtttacct gatggttacg gtcgtggtag aatcattgct
     6841 gactacagaa gaattgcttt gtacggtgtt gatgctttga tccaattgaa acaagctgac
     6901 tttgccgctg tcaccggttc ttctgtcgaa gccatgagat tgagatctga aatcttccac
     6961 caaatcaagg ctttgaagga attgttggtc atggctgact cttacggtgt cgatttgaga
     7021 caaccagcct ctactttcaa ggatgctgct caagctttat ggttgggtca cactgctgct
     7081 ttgaaggaac aagacggtgc tgccatgtct gccggtagat gggactcttt cttggacatc
     7141 tacgctgaaa aggacttggc tgctggtgtt gctactgaag aagatattca agaagttgtt
     7201 gatgacatgg ttttgaagat gagattagtc agacacgtta gaaccccaga atacaacgaa
     7261 ttattcaccg gtgacccaac ctggaccacc ttggctttag gtggtgctac tgaagattcc
     7321 aagtccatgg tcaccaagac cacctacaga tttttgcact ctttgagaaa cttgggtcca
     7381 gctccagaac caaacttgac cgtcttatgg tccaaggtcc actcttccca cttcaaggaa
     7441 tactgtgctc aattgtccat tgacacttct tccatccaat acgaaaacga cgatttgatg
     7501 cgtccaatct tcggttctga ctacgccatt gcctgttgtg tttctgccat gagaatcggt
     7561 actgacatgc aattcttcgg tgccagaacc aacttggcca aattgctatt gatggttttg
     7621 aacggtggta gagatgaatt ggaaggtcaa ttattgtctg aaccattggc tgaagcttgt
     7681 cgtgaagctg gtatcggtgc tggtgacgaa gataccccat tagattacga aaaggtttcc
     7741 aagctatatt tcgacgttgc catcccatgg atggctgaat tatacgctga caccatgaac
     7801 tgtattcact actcccacga cgctacttct tacgaaaact tacaaatggc tttgcacaac
     7861 tctaacgtta accatttcat ggctttcggt attgctggtt tgtctgttgt tgctgactct
     7921 ttggctgcta tcaaatacga tgacgtctac ccaatcagaa acgaaaacgg tttgaccgtc
     7981 ggtttcaagc gtcgtcaccc agaatccgtc ttgccaactt tcggtaacga caacccaaag
     8041 gtcgatgaca ttgctgttga agttgctgac gctttctacg aagaattgaa caagcaaaaa
     8101 ttatacaagg atgctaaggc cactgtctcc gtcttgacca ttacctccaa tgtcgtttac
     8161 ggtaaatcca ctggtactac tccagatggt agaatcattg gtgaaccatt tgctccaggt
     8221 gccaacccaa tgcacaacag agacagaaac ggtgccattg cttctttggc ctccgttgcc
     8281 aagttgccat acgcttcttg tatggacggt atctccaaca ctttctgttt gaccccaact
     8341 gctttgggtt ccatggctga cgttagagct atgaacttgg tttctctatt ggacggttac
     8401 ttcggtaaga acggtcacca tttgaacatc aacgttttga acagagaatt gttggaagat
     8461 gctcatttgc acccagaaaa atacccagac ttgaccatca gagtctctgg ttacgctgtc
     8521 agattcaacc aattgactcc agaacaaaga gaagaagttt tgaagagaac tatgtaagga
     8581 gttaaaggca aagttttctt ttctagagcc gttcccacaa ataattatac gtatatgctt
     8641 cttttcgttt actatatatc tatatttaca agcctttatt cactgatgca atttgtttcc
     8701 aaatactttt ttggagatct cataactaga tatgatgatg gcgcaacttg ggcgtatctt
     8761 aattactctg gctgccaggc ccgtgtagag ggccgcaaga ccttctgtac gccatatagt
     8821 ctctaagaac ttgaacatgt ttctagacct attgccgcct ttcggatcgc tattgttcat
     8881 catggatatt tgccatctcg tcttaccgac atcaaaaggg tgtgtgcata tagcagctat
     8941 catcccactt atgcaaccac tggcaaaact gtttataaaa tggacccagt ttgcgtcctt
     9001 agatgcaaat cgagtagaat ctagccatag tctttccttg caaagttcat aggaactcca
     9061 atatattgca ctaaacggaa ttcactggta gagagcgact ttgtatgccc caattgcgaa
     9121 acccgcgtta tccttctcga ttctttagta cccgaccagg acaaggaaaa ggaggtcgaa
     9181 acgtttttga agaaacaaga ggaactacac ggaagctcta aagatggcaa ccagccagaa
     9241 actaagaaaa tgaagttgat ggttccaact ggcaccgctg gcttgaacaa caataccagc
     9301 cttccaactt ctgtaaataa cggcggtacg ccagtgccac cagtaccgtt acctttcggt
     9361 atacctcctt tccccatgtt tccaatgccc ttcatgcctc caacggctac tatcacaaat
     9421 cctcatcaag ctgacgcaag ccctaagaaa tgaataacaa tactgacagt actaaataat
     9481 tgcctacttg gcttcacata cgttgcatac gtcgatatag ataataatga taatgacagc
     9541 aggattatcg taatacgtaa tagttgaaaa tctcaaaaat gtgtgggtca ttacgtaaat
     9601 aatgatagga atgggattct tctatttttc ctttttccat tctagcagcc gtcgggaaaa
     9661 cgtggcatcc tctctttcgg gctcaattgg agtcacgctg ccgtgagcat cctctctttc
     9721 catatctaac aactgagcac gtaaccaatg gaaaagcatg agcttagcgt tgctccaaaa
     9781 aagtattgga tggttaatac catttgtctg ttctcttctg actttgactc ctcaaaaaaa
     9841 aaaaatctac aatcaacaga tcgcttcaat tacgccctca caaaaacttt tttccttctt
     9901 cttcgcccac gttaaatttt atccctcatg ttgtctaacg gatttctgca cttgatttat
     9961 tataaaaaga caaagacata atacttctct atcaatttca gttattgttc ttccttgcgt
    10021 tattcttctg ttcttctttt tcttttgtca tatataacca taaccaagta atacatattc
    10081 aaaatgatca ttggttctgt tcactctttg gaaactttca cctccaacga tggtccaggt
    10141 atcagaactt tggttttctt gcaaggttgt tccaagagat gtatgtactg ttccaaccca
    10201 gaaactcaat gtattgtcga cccattctct tgtccagaag ttgctgtctc tgatgaagaa
    10261 gttgctaacg ttttgaagcg ttacgaacac ttcttaagac caaacaatgg tggtatcact
    10321 ttctccggtg gtgaaccatt attgcaacca aactttgtcg gttctgtttt caagaaggcc
    10381 aagtccattg gtttgaccac ctgtgttgac acttctggtc acggtaaccc agccatctgg
    10441 aacaaatgtc taccacacac cgattacgtc atgttgtgtt tgaagggtat ggacatgaag
    10501 ttggcttctt tcatctctgg tgtctctgaa tccaacaacc aaagagctag agaatttgcc
    10561 aagtacgtca gagacaacta caagaacatc aaattgtcca tcagatgggt tttgttgaag
    10621 gacatgactg acaccgatga agaattggat gctttggctg ctttcaccaa ggacttgtct
    10681 ccagttttca cccacgtcga attgttgcca taccatattt tgggtaagga caaatacgaa
    10741 gctatgaact ctttataccc aatggaaggt atggagcctt aagctcctgt tgaagtagca
    10801 tttaatcata atttttgtca cattttaatc aacttgattt ttctggttta atttttctaa
    10861 ttttaatttt aattttttta tcaatgggaa ctgatacact aaaaagaatt aggagccaac
    10921 aagaataagc cgcttatttc ctactagagt ttgcttaaaa tttcatctcg aattgtcatt
    10981 ctaatatttt atccacacac acaccttaaa atttttagat taaatggcat caactcttag
    11041 cttcacacac acacacacac cgaagctggt tgttttattt gatttgatat aattggtttc
    11101 tctggatggt actttttctt tcttggttat ttcctatttt aaaatatgaa acgcacacaa
    11161 gtcataatta ttctaataga gcacaattca caacacgcac atttcaactt taatattttt
    11221 ttagaaacac tttatttagt ctaattctta atttttaata tatataatgc acacacacgt
    11281 ttggatccac tagttctaga gcggccgcca ccgcggtgga gctccagctt ttgttccctt
    11341 tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
    11401 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
    11461 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
    11521 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
    11581 ttgcgtattg ggcgc
//
