LOCUS       40924_45423        9307 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pUDE150, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9307)
  AUTHORS   Kozak BU, van Rossum HM, Benjamin KR, Wu L, Daran JM, Pronk JT, van 
            Maris AJ.
  TITLE     Replacement of the Saccharomyces cerevisiae acetyl-CoA synthetases 
            by alternative pathways for cytosolic acetyl-CoA synthesis
  JOURNAL   Metab. Eng. (2013) In press
  PUBMED    24269999
REFERENCE   2  (bases 1 to 9307)
  AUTHORS   Kozak BU, van Rossum HM, Benjamin KR, Wu L, Daran J-M.G., Pronk JT, 
            van Maris AJA.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAY-2013) Biotechnology, Delft University of 
            Technology, Julianalaan 67, Delft 2628BC, The Netherlands
REFERENCE   3  (bases 1 to 9307)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9307)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab. Eng.
            (2013) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-MAY-2013) Biotechnology, Delft University of Technology, 
            Julianalaan 67, Delft 2628BC, The Netherlands"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9307
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(183..771)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(945..1802)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1803..1907)
                     /label=AmpR promoter
     rep_origin      complement(1934..3276)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        3540..3760
                     /label=URA3 promoter
     CDS             3761..4561
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(4695..5150)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     5295..5311
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5321..5339
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(5358..5605)
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     5608..5624
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5657..5681
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(5697..8306)
                     /codon_start=1
                     /gene="adhE"
                     /product="alcohol-acetaldehyde dehydrogenase"
                     /label=adhE
                     /protein_id="AHB33295.1"
                     /translation="MLTIPEKENRGSKEQEVAIMIDALADKGKKALEALSKKSQEEIDH
                     IVHQMSLAAVDQHMVLAKLAHEETGRGIYEDKAIKNLYASEYIWNSIKDNKTVGIIGED
                     KEKGLTYVAEPIGVICGVTPTTNPTSTTIFKAMIAIKTGNPIIFAFHPSAQESSKRAAE
                     VVLEAAMKAGAPKDIIQWIEVPSIEATKQLMNHKGIALVLATGGSGMVKSAYSTGKPAL
                     GVGPGNVPSYIEKTAHIKRAVNDIIGSKTFDNGMICASEQVVVIDKEIYKDVTNEFKAH
                     QAYFVKKDELQRLENAIMNEQKTGIKPDIVGKSAVEIAELAGIPVPENTKLIIAEISGV
                     GSDYPLSREKLSPVLALVKAQSTKQAFQICEDTLHFGGLGHTAVIHTEDETLQKDFGLR
                     MKACRVLVNTPSAVGGIGDMYNELIPSLTLGCGSYGRNSISHNVSATDLLNIKTIAKRR
                     NNTQIFKVPAQIYFEENAIMSLTTMDKIEKVMIVCDPGMVEFGYTKTVENVLRQRTEQP
                     QIKIFSEVEPNPSTNTVYKGLEMMVDFQPDTIIALGGGSAMDAAKAMWMFFEHPETSFF
                     GAKQKFLDIGKRTYKIGMPENATFICIPTTSGTGSEVTPFAVITDSETNVKYPLADFAL
                     TPDVAIIDPQFVMSVPKSVTADTGMDVLTHAMESYVSVMASDYTRGLSLQAIKLTFEYL
                     KSSVEKGDKVSREKMHNASTLAGMAFANAFLGIAHSIAHKIGGEYGIPHGRANAILLPH
                     IIRYNAKDPQKHALFPKYEFFRADTDYADIAKFLGLKGNTTEALVESLAKAVYELGQSV
                     GIEMNLKSQGVSEEELNESIDRMAELAFEDQCTTANPKEALISEIKDIIQTSYDYKQ"
     gene            complement(5697..8306)
                     /gene="adhE"
                     /label=adhE
     protein_bind    complement(8322..8346)
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(8354..8370)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(8376..9020)
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate
                     dehydrogenase; also known as the TDH3 promoter"
     promoter        complement(9049..9067)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9088..9104)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(9112..9128)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9136..9166)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(9181..9202)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
ORIGIN
        1 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
       61 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
      121 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      181 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      241 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      301 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      361 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      421 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
      481 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
      541 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
      601 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
      661 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
      721 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
      781 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
      841 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
      901 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
      961 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     1021 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     1081 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     1141 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     1201 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     1261 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     1321 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     1381 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     1441 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     1501 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     1561 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     1621 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     1681 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     1741 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     1801 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     1861 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     1921 aaagtgccac ctgaacgaag catctgtgct tcattttgta gaacaaaaat gcaacgcgag
     1981 agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc
     2041 gaaagcgcta ttttaccaac gaagaatctg tgcttcattt ttgtaaaaca aaaatgcaac
     2101 gcgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc
     2161 aacgcgagag cgctatttta ccaacaaaga atctatactt cttttttgtt ctacaaaaat
     2221 gcatcccgag agcgctattt ttctaacaaa gcatcttaga ttactttttt tctcctttgt
     2281 gcgctctata atgcagtctc ttgataactt tttgcactgt aggtccgtta aggttagaag
     2341 aaggctactt tggtgtctat tttctcttcc ataaaaaaag cctgactcca cttcccgcgt
     2401 ttactgatta ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat ccccgattat
     2461 attctatacc gatgtggatt gcgcatactt tgtgaacaga aagtgatagc gttgatgatt
     2521 cttcattggt cagaaaatta tgaacggttt cttctatttt gtctctatat actacgtata
     2581 ggaaatgttt acattttcgt attgttttcg attcactcta tgaatagttc ttactacaat
     2641 ttttttgtct aaagagtaat actagagata aacataaaaa atgtagaggt cgagtttaga
     2701 tgcaagttca aggagcgaaa ggtggatggg taggttatat agggatatag cacagagata
     2761 tatagcaaag agatactttt gagcaatgtt tgtggaagcg gtattcgcaa tattttagta
     2821 gctcgttaca gtccggtgcg tttttggttt tttgaaagtg cgtcttcaga gcgcttttgg
     2881 ttttcaaaag cgctctgaag ttcctatact ttctagagaa taggaacttc ggaataggaa
     2941 cttcaaagcg tttccgaaaa cgagcgcttc cgaaaatgca acgcgagctg cgcacataca
     3001 gctcactgtt cacgtcgcac ctatatctgc gtgttgcctg tatatatata tacatgagaa
     3061 gaacggcata gtgcgtgttt atgcttaaat gcgtacttat atgcgtctat ttatgtagga
     3121 tgaaaggtag tctagtacct cctgtgatat tatcccattc catgcggggt atcgtatgct
     3181 tccttcagca ctacccttta gctgttctat atgctgccac tcctcaattg gattagtctc
     3241 atccttcaat gctatcattt cctttgatat tggatcatac taagaaacca ttattatcat
     3301 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
     3361 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     3421 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     3481 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata ccacagcttt
     3541 tcaattcaat tcatcatttt ttttttattc ttttttttga tttcggtttc tttgaaattt
     3601 ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca gacttagatt
     3661 ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat tcttaaccca
     3721 actgcacaga acaaaaacct gcaggaaacg aagataaatc atgtcgaaag ctacatataa
     3781 ggaacgtgct gctactcatc ctagtcctgt tgctgccaag ctatttaata tcatgcacga
     3841 aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc accaaggaat tactggagtt
     3901 agttgaagca ttaggtccca aaatttgttt actaaaaaca catgtggata tcttgactga
     3961 tttttccatg gagggcacag ttaagccgct aaaggcatta tccgccaagt acaatttttt
     4021 actcttcgaa gacagaaaat ttgctgacat tggtaataca gtcaaattgc agtactctgc
     4081 gggtgtatac agaatagcag aatgggcaga cattacgaat gcacacggtg tggtgggccc
     4141 aggtattgtt agcggtttga agcaggcggc agaagaagta acaaaggaac ctagaggcct
     4201 tttgatgtta gcagaattgt catgcaaggg ctccctatct actggagaat atactaaggg
     4261 tactgttgac attgcgaaga gcgacaaaga ttttgttatc ggctttattg ctcaaagaga
     4321 catgggtgga agagatgaag gttacgattg gttgattatg acacccggtg tgggtttaga
     4381 tgacaaggga gacgcattgg gtcaacagta tagaaccgtg gatgatgtgg tctctacagg
     4441 atctgacatt attattgttg gaagaggact atttgcaaag ggaagggatg ctaaggtaga
     4501 gggtgaacgt tacagaaaag caggctggga agcatatttg agaagatgcg gccagcaaaa
     4561 ctaaaaaact gtattataag taaatgcatg tatactaaac tcacaaatta gagcttcaat
     4621 ttaattatat cagttattac cctatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     4681 aataccgcat caggaaattg taaacgttaa tattttgtta aaattcgcgt taaatttttg
     4741 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     4801 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     4861 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     4921 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     4981 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     5041 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     5101 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cgcgccattc
     5161 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     5221 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     5281 ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg
     5341 cgaattgggt accggccgca aattaaagcc ttcgagcgtc ccaaaacctt ctcaagcaag
     5401 gttttcagta taatgttaca tgcgtacacg cgtctgtaca gaaaaaaaag aaaaatttga
     5461 aatataaata acgttcttaa tactaacata actataaaaa aataaatagg gacctagact
     5521 tcaggttgtc taactccttc cttttcggtt agagcggatg tggggggagg gcgtgaatgt
     5581 aagcgtgaca taactaatta catgactcga ggtcgacggt atcgataagc ttgatatcga
     5641 attcctgcag cccatcacca ctttgtacaa gaaagctggg tgtcgactag gaattcttat
     5701 tgcttgtagt cgtaagaagt ttggatgatg tccttgattt cagagatcaa agcttccttt
     5761 gggttagcag tagtacattg gtcttcgaaa gccaattcag ccattctgtc gatagattcg
     5821 ttcaattctt cttcagaaac accttgagac ttcaagttca tttcgatacc aacagattga
     5881 cccaattcgt aaacagcctt agccaaagat tcaaccaaag cttcagtagt gttacccttc
     5941 aaacccaaga acttagcgat gtcagcgtag tcagtgtcag ctctgaaaaa ttcgtacttt
     6001 gggaacaaag cgtgcttttg tgggtcctta gcgttgtatc tgatgatgtg tggcaacaag
     6061 atagcgttag ctctaccgtg tgggataccg tattcaccac cgatcttgtg agcgatagag
     6121 tgagcgatac ccaagaaagc gttagcgaaa gccataccag ccaaagtaga agcgttgtgc
     6181 atcttttctc tagaaacctt gtcacccttt tcaacagaag acttcaagta ttcgaaagtc
     6241 aacttgatag cttgcaaaga caaacctcta gtgtagtcag aagccataac agaaacgtaa
     6301 gattccatag cgtgagtcaa aacgtccata ccagtgtcag cagtaacaga ctttggaaca
     6361 gacataacga attgtgggtc gatgatagca acgtctggag tcaaagcgaa gtcagccaat
     6421 gggtacttaa cgttagtttc agagtcagtg ataacagcga atggagtaac ttcagaacca
     6481 gtaccagaag tagttgggat acagatgaaa gtagcgtttt ctggcatacc gatcttgtaa
     6541 gttctcttac cgatgtccaa gaacttttgc ttagcaccga agaaagaagt ttctgggtgt
     6601 tcgaagaaca tccacatagc cttagcagcg tccatagcag aaccaccacc caaagcgatg
     6661 atagtgtctg gttggaagtc aaccatcatt tccaaaccct tgtaaacagt gttagtagat
     6721 gggtttggtt caacttcaga gaagatcttg atttgtggtt gttcagttct ttgtctcaaa
     6781 acgttttcaa cagtcttagt gtaaccaaat tcaaccatac ctgggtcaca aacgatcata
     6841 accttttcga tcttgtccat agtagtcaaa gacatgatag cgttttcttc gaagtagatt
     6901 tgagctggaa ccttgaagat ttgagtgttg tttcttctct tagcgatagt cttgatgttc
     6961 aacaagtcag tagcagaaac gttgtgagag atagagtttc taccgtaaga accacaaccc
     7021 aaagtcaaag atgggatcaa ttcgttgtac atgtcaccga taccaccaac agcagatgga
     7081 gtgttaacca aaactctaca agccttcatt ctcaaaccga agtccttttg caaagtttcg
     7141 tcttcagtgt ggataacagc agtgtgaccc aaaccaccga agtgcaaagt gtcttcacag
     7201 atttggaaag cttgcttagt agattgagcc ttaaccaaag ccaaaactgg agacaacttt
     7261 tctctagaca atgggtagtc agaaccaaca ccagagattt cagcgatgat caacttagtg
     7321 ttttctggaa ctgggatacc agccaattca gcgatttcaa cagcagactt accaacgatg
     7381 tctggcttga taccagtctt ttgttcgttc atgatagcgt tttccaatct ttgcaattcg
     7441 tccttcttaa cgaagtaagc ttggtgagcc ttaaattcgt tagtaacgtc cttgtagatt
     7501 tccttgtcga taacaacaac ttgttcagaa gcacagatca taccgttgtc gaaagtctta
     7561 gaaccgatga tgtcgttaac agctctcttg atgtgagcag tcttttcgat gtaagatgga
     7621 acgttacctg gaccaacacc caaagctggc ttaccagtag agtaagcaga cttaaccata
     7681 ccagaaccac cagtagccaa aaccaaagcg atacccttgt ggttcatcaa ttgcttagta
     7741 gcttcgatag atggaacttc gatccattgg atgatgtcct ttggagcacc agccttcata
     7801 gcagcttcca aaacaacttc agcagctctc ttagaagatt cttgagcaga tgggtggaaa
     7861 gcgaagatga ttgggttacc agtcttgata gcgatcatag ccttgaagat agtagtagaa
     7921 gttgggttag tagttggagt aacaccacag ataacaccga ttggttcagc aacgtaagtc
     7981 aaaccctttt ccttgtcttc accgatgata ccaacagtct tgttgtcctt gatagagttc
     8041 cagatgtatt cagaagcgta caagttcttg atagccttgt cttcgtagat acctctacca
     8101 gtttcttcgt gagccaactt agccaaaacc atgtgttggt caacagcagc caaagacatt
     8161 tggtgaacga tgtggtcgat ttcttcttga gacttcttag acaaagcttc caaagccttc
     8221 ttacccttgt cagccaaagc gtcgatcatg atagcaactt cttgttcctt agaacctctg
     8281 ttttcctttt ctgggatagt caacatggat cctatctgca gagcctgctt ttttgtacaa
     8341 acttgtgatg ggggatccac tagttctaga atccgtcgaa actaagttct ggtgttttaa
     8401 aactaaaaaa aagactaact ataaaagtag aatttaagaa gtttaagaaa tagatttaca
     8461 gaattacaat caatacctac cgtctttata tacttattag tcaagtaggg gaataatttc
     8521 agggaactgg tttcaacctt ttttttcagc tttttccaaa tcagagagag cagaaggtaa
     8581 tagaaggtgt aagaaaatga gatagataca tgcgtgggtc aattgccttg tgtcatcatt
     8641 tactccaggc aggttgcatc actccattga ggttgtgccc gttttttgcc tgtttgtgcc
     8701 cctgttctct gtagttgcgc taagagaatg gacctatgaa ctgatggttg gtgaagaaaa
     8761 caatattttg gtgctgggat tctttttttt tctggatgcc agcttaaaaa gcgggctcca
     8821 ttatatttag tggatgccag gaataaacct gttcacccag gcaccttcgg tgttatatat
     8881 tctgtgtaac ccgcccccta ttttgggcat gtacgggtta cagcagaatt aaaaggctaa
     8941 ttttttgact aaataaagtt aggaaaatca ctactattaa ttatttacgt attctttgaa
     9001 atggcgagta ttgataatga taaactcgaa gagctccagc ttttgttccc tttagtgagg
     9061 gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     9121 gctcacaatt ccacacaaca taggagccgg aagcataaag tgtaaagcct ggggtgccta
     9181 atgagtgagg taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     9241 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     9301 tgggcgc
//
