LOCUS       V002349                10005 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002349
VERSION     V002349
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10005)
  AUTHORS   Beekwilder J, van Rossum H.
  TITLE     Production of carotenoids in Saccharomyces cerevisiae by a
            self-processing polyprotein and conversion to beta-ionone
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10005)
  AUTHORS   Beekwilder J, van Rossum H.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2013) BU Bioscience, Plant Research International,
            P.O.Box 619, Wageningen, Gelderland 6700AP, Netherlands
REFERENCE   3  (bases 1 to 10005)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10005)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: IDBA v. 1
            Coverage              :: 160
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (13-SEP-2013) BU Bioscience, Plant Research International, P.O.Box
            619, Wageningen, Gelderland 6700AP, Netherlands"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10005
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..650
                     /label="GAP promoter"
                     /note="promoter for glyceraldehyde-3-phosphate
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     656..672
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             674..2692
                     /gene="crtYB"
                     /label="Bifunctional lycopene cyclase/phytoene synthase"
                     /note="Bifunctional lycopene cyclase/phytoene synthase from
                     Phaffia rhodozyma. Accession#: Q7Z859"
     CDS             2699..2752
                     /label="T2A"
                     /note="2A peptide from Thosea asigna virus capsid protein"
     misc_feature    2753..4495
                     /note="phytoene desaturase from Xanthophyllomyces
                     dendrorhous; Region: CrtI"
     misc_feature    4496..4555
                     /label="Region: T2A ribosomal skipping sequence"
                     /note="Region: T2A ribosomal skipping sequence"
     CDS             4502..4555
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid
                     protein"
                     /label="T2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5091..5102
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     terminator      5748..5945
                     /label="TEF terminator"
                     /note="Ashbya gossypii TEF terminator"
     CDS             6439..7242
                     /codon_start=1
                     /product="orotidine-5'-phosphate decarboxylase"
                     /label="orotidine-5'-phosphate decarboxylase"
                     /note="URA3; derived from Kluyveromyces lactis"
                     /protein_id="AHZ97959.1"
                     /translation="MSTKSYTSRAETHASPVASKLLRLMDEKKTNLCASLDVRSTDELL
                     KLVETLGPYICLLKTHVDILDDFSYEGTVVPLKALAEKYKFLIFEDRKFADIGNTVKLQ
                     YTSGVYRIAEWSDITNAHGVTGAGIVAGLKQGAQEVTKEPRGLLMLAELSSKGSLAHGE
                     YTKGTVDIAKSDKDFVIGFIAQNDMGGREEGFDWLIMTPGVGLDDKGDALGQQYRTVDE
                     VVSGGSDIIIVGRGLFAKGRDPKVEGERYRNAGWEAYQKRISAPH"
     promoter        7426..7530
                     /label="AmpR promoter"
     CDS             7531..8388
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      8562..9150
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(9422..9925)
                     /label="CEN/ARS"
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
ORIGIN
        1 agtttatcat tatcaatact cgccatttca aagaatacgt aaataattaa tagtagtgat
       61 tttcctaact ttatttagtc aaaaaattag ccttttaatt ctgctgtaac ccgtacatgc
      121 ccaaaatagg gggcgggtta cacagaatat ataacatcgt aggtgtctgg gtgaacagtt
      181 tattcctggc atccactaaa tataatggag cccgcttttt aagctggcat ccagaaaaaa
      241 aaagaatccc agcaccaaaa tattgttttc ttcaccaacc atcagttcat aggtccattc
      301 tcttagcgca actacagaga acaggggcac aaacaggcaa aaaacgggca caacctcaat
      361 ggagtgatac aacctgcctg gagtaaatga tgacacaagg caattgaccc acgcatgtat
      421 ctatctcatt ttcttacacc ttctattacc ttctgctctc tctgatttgg aaaaagctga
      481 aaaaaaaggt tgaaaccagt tccctgaaat tattccccta cttgactaat aagtatataa
      541 agacggtagg tattgattgt aattctgtaa atctatttct taaacttctt aaattctact
      601 tttatagtta gtcttttttt tagttttaaa acaccagaac ttagtttcga cggattctag
      661 aactagtgga tccatgacgg ctctcgcata ttaccagatc catctgatct atactctccc
      721 aattcttggt cttctcggcc tgctcacttc cccgattttg acaaaatttg acatctacaa
      781 aatatcgatc ctcgtattta ttgcgtttag tgcaaccaca ccatgggact catggatcat
      841 cagaaatggc gcatggacat atccatcagc ggagagtggc caaggcgtgt ttggaacgtt
      901 tctagatgtt ccatatgaag agtacgcttt ctttgtcatt caaaccgtaa tcaccggctt
      961 ggtctacgtc ttggcaacta ggcaccttct cccatctctc gcgcttccca agactagatc
     1021 gtccgccctt tctctcgcgc tcaaggcgct catccctctg cccattatct acctatttac
     1081 cgctcacccc agcccatcgc ccgacccgct cgtgacagat cactacttct acatgcgggc
     1141 actctcctta ctcatcaccc cacctaccat gctcttggca gcattatcag gcgaatatgc
     1201 tttcgattgg aaaagtggcc gagcaaagtc aactattgca acaatcatga tcccgacggt
     1261 gtatctgatt tgggtagatt atgttgctgt cggtcaagac tcttggtcga tcaacgatga
     1321 gaagattgta gggtggaggc ttggaggtgt actacccatt gaggaagcta tgttcttctt
     1381 actgacgaat ctaatgattg ttctgggtct gtctgcctgc gatcatactc aggccctata
     1441 cctgctacac ggtcgaacta tttatggcaa caaaaagatg ccatcttcat ttcccctcat
     1501 tacaccgcct gtgctctccc tgttttttag cagccgacca tactcttctc agccaaaacg
     1561 tgacttggaa ctggcagtca agttgttgga gaaaaagagc cggagctttt ttgttgcctc
     1621 ggctggattt cctagcgaag ttagggagag gctggttgga ctatacgcat tctgccgggt
     1681 gactgatgat cttatcgact ctcctgaagt atcttccaac ccgcatgcca caattgacat
     1741 ggtctccgat tttcttaccc tactatttgg gcccccgcta cacccttcgc aacctgacaa
     1801 gatcctttct tcgcctttac ttcctccttc gcacccttcc cgacccacgg gaatgtatcc
     1861 cctcccgcct cctccttcgc tctcgcctgc cgagctcgtt caattcctta ccgaaagggt
     1921 tcccgttcaa taccatttcg ccttcaggtt gctcgctaag ttgcaagggc tgatccctcg
     1981 atacccactc gacgaactcc ttagaggata caccactgat cttatctttc ccttatcgac
     2041 agaggcagtc caggctcgga agacgcctat cgagaccaca gctgacttgc tggactatgg
     2101 tctatgtgta gcaggctcag tcgccgagct attggtctat gtctcttggg caagtgcacc
     2161 aagtcaggtc cctgccacca tagaagaaag agaagctgtg ttagtggcaa gccgagagat
     2221 gggaactgcc cttcagttgg tgaacattgc tagggacatt aaaggggacg caacagaagg
     2281 gagattttac ctaccactct cattctttgg tcttcgggat gaatcaaagc ttgcgatccc
     2341 gactgattgg acggaacctc ggcctcaaga tttcgacaaa ctcctcagtc tatctccttc
     2401 gtccacatta ccatcttcaa acgcctcaga aagcttccgg ttcgaatgga agacgtactc
     2461 gcttccatta gtcgcctacg cagaggatct tgccaaacat tcttataagg gaattgaccg
     2521 acttcctacc gaggttcaag cgggaatgcg agcggcttgc gcgagctacc tactgatcgg
     2581 ccgagagatc aaagtcgttt ggaaaggaga cgtcggagag agaaggacag ttgccggatg
     2641 gaggagagta cggaaagtct tgagtgtggt catgagcgga tgggaagggc agagagcaga
     2701 aggaaggggt tctttgttga cttgtggaga tgttgaggag aatccaggac caggaaaaga
     2761 acaagatcag gataaaccca cagctatcat cgtgggatgt ggtatcggtg gaatcgccac
     2821 tgccgctcgt cttgctaaag aaggtttcca ggtcacggtg ttcgagaaga acgactactc
     2881 cggaggtcga tgctctttaa tcgagcgaga tggttatcga ttcgatcagg ggcccagttt
     2941 gctgctcttg ccagatctct tcaagcagac attcgaagat ttgggggaga agatggaaga
     3001 ttgggtcgat ctcatcaagt gtgaacccaa ctatgtttgc cacttccacg atgaagagac
     3061 tttcactttt tcaaccgaca tggcgttgct caagcgggaa gtcgagcgtt ttgaaggcaa
     3121 agatggattt gatcggttct tgtcgtttat ccaagaagcc cacagacatt acgagcttgc
     3181 tgtcgttcac gtcctgcaga agaacttccc tggcttcgca gcattcttac ggctacagtt
     3241 cattggccaa atcctggctc ttcacccctt cgagtctatc tggacaagag tttgtcgata
     3301 tttcaagacc gacagattac gaagagtctt ctcgtttgca gtgatgtaca tgggtcaaag
     3361 cccatacagt gcgcccggaa catattcctt gctccaatac accgaattga ccgagggcat
     3421 ctggtatccg agaggaggct tttggcaggt tcctaatact cttcttcaga tcgtcaagcg
     3481 caacaatccc tcagccaagt tcaatttcaa cgctccagtt tcccaggttc ttctctctcc
     3541 tgccaaggac cgagcgactg gtgttcgact tgaatccggc gaggaacatc acgccgatgt
     3601 tgtgattgtc aatgctgacc tcgtttacgc ctccgagcac ttgattcctg acgatgccag
     3661 aaacaagatt ggccaactgg gtgaagtcaa gagaagttgg tgggctgact tagttggtgg
     3721 aaagaagctc aagggaagtt gcagtagttt gagcttctac tggagcatgg accgaatcgt
     3781 ggacggtctg ggcggacaca atatcttctt ggccgaggac ttcaagggat cattcgacac
     3841 aatcttcgag gagttgggtc tcccagccga tccttccttt tacgtgaacg ttccctcgcg
     3901 aatcgatcct tctgccgctc ccgaaggcaa agatgctatc gtcattcttg tgccgtgtgg
     3961 ccatatcgac gcttcgaacc ctcaagatta caacaagctt gttgctcggg caaggaagtt
     4021 tgtgatccaa acgctttccg ccaagcttgg acttcccgac tttgaaaaaa tgattgtggc
     4081 agagaaggtt cacgatgctc cctcttggga gaaagaattt aacctcaagg acggaagcat
     4141 cttgggactg gctcacaact ttatgcaagt tcttggtttc aggccgagca ccagacatcc
     4201 caagtatgac aagttgttct ttgtcggggc ttcgactcat cccggaactg gggttcccat
     4261 cgtcttggct ggagccaagt taactgccaa ccaagttctc gaatcctttg accgatcccc
     4321 agctccagat cccaatatgt cactctccgt accatatgga aaacctctca aatcaaatgg
     4381 aacgggtatc gattctcagg tccagctgaa gttcatggat ttggagagat gggtatacct
     4441 tttggtgttg ttgattgggg ccgtgatcgc tcgatccgtt ggtgttcttg ctttcagagc
     4501 tgagggtaga ggaagtctac taacatgcgg tgacgtagaa gaaaaccctg gtcctgatta
     4561 cgcgaacatc ctcacagcaa ttccactcga gtttactcct caggatgata tcgtgctcct
     4621 tgaaccgtat cactacctag gaaagaaccc tggaaaagaa attcgatcac aactcatcga
     4681 ggctttcaac tattggttgg atgtcaagaa ggaggatctc gaggtcatcc agaacgttgt
     4741 tggcatgcta cataccgcta gcttattaat ggacgatgtg gaggattcat cggtcctcag
     4801 gcgtgggtcg cctgtggccc atctaattta cgggattccg cagacaataa acactgcaaa
     4861 ctacgtctac tttctggctt atcaagagat cttcaagctt cgcccaacac cgatacccat
     4921 gcctgtaatt cctccttcat ctgcttcgct tcaatcatcc gtctcctctg catcctcctc
     4981 ctcctcggcc tcgtctgaaa acgggggcac gtcaactcct aattcgcaga ttccgttctc
     5041 gaaagatacg tatcttgata aagtgatcac agacgagata ctttccctcc atagagggca
     5101 gggcctggag ctattctgga gagatagtct gacgtgtcct agcgaagagg aatatgtgaa
     5161 aatggttctt ggaaagacgg gaggtttgtt ccgtatagcg gtcagattga tgatggcaaa
     5221 gtcagaatgt gacatagact ttgtccagct tgtcaacttg atctcaatat acttccagat
     5281 cagggatgac tatatgaacc ttcagtcttc tgagtatgcc cataataaga attttgcaga
     5341 ggacctcaca gaagggaaat tcagttttcc cactatccac tcgattcatg ccaacccctc
     5401 atcgagactc gtcatcaata cgttgcagaa gaaatcgacc tctcctgaga tccttcacca
     5461 ctgtgtaaac tacatgcgca cagaaaccca ctcattcgaa tatactcagg aagtcctcaa
     5521 caccttgtca ggtgcactcg agagagaact aggaaggctt caaggagagt tcgcagaagc
     5581 taactcaagg atggatcttg gagacgtaga ttcggaagga agaacgggga agaacgtcaa
     5641 attggaagcg atcctgaaaa agctagccga tatccctctg tgagtcgacc tcgagcggaa
     5701 gggcgaattc tgcagatatc catcacactg gcggccgctc gagcagtact gacaataaaa
     5761 agattcttgt tttcaagaac ttgtcatttg tatagttttt ttatattgta gttgttctat
     5821 tttaatcaaa tgttagcgtg atttatattt tttttcgcct cgacatcatc tgcccagatg
     5881 cgaagttaag tgcgcagaaa gtaatatcat gcgtcaatcg tatgtgaatg ctggtcgcta
     5941 tactggggaa ttcgcggtac cctggttgaa cattcttagg ctggtcgaat catttagaca
     6001 cgggcatcgt cctctcgaaa ggtgctcaga agctcatcga actgtcatcc tgcgtgaaga
     6061 ttaaaatcca acttagaaat ttcgagctta cggagacaat catatgggag aagcaattgg
     6121 aagatagaaa aaaggtactc ggtacataaa tatatgtgat tctgggtaga agatcggtct
     6181 gcattggatg gtggtaacgc atttttttac acacattact tgcctcgagc atcaaatggt
     6241 ggttattcgt ggatctatat cacgtgattt gcttaagaat tgtcgttcat ggtgacactt
     6301 ttagctttga catgattaag ctcatctcaa ttgatgttat ctaaagtcat ttcaactatc
     6361 taagatgtgg ttgtgattgg gccattttgt gaaagccagt acgccagcgt caatacactc
     6421 ccgtcaatta gttgcaccat gtccacaaaa tcatatacca gtagagctga gactcatgca
     6481 agtccggttg catcgaaact tttacgttta atggatgaaa agaagaccaa tttgtgtgct
     6541 tctcttgacg ttcgttcgac tgatgagcta ttgaaacttg ttgaaacgtt gggtccatac
     6601 atttgccttt tgaaaacaca cgttgatatc ttggatgatt tcagttatga gggtactgtc
     6661 gttccattga aagcattggc agagaaatac aagttcttga tatttgagga cagaaaattc
     6721 gccgatatcg gtaacacagt caaattacaa tatacatcgg gcgtttaccg tatcgcagaa
     6781 tggtctgata tcaccaacgc ccacggggtt actggtgctg gtattgttgc tggcttgaaa
     6841 caaggtgcgc aagaggtcac caaagaacca aggggattat tgatgcttgc tgaattgtct
     6901 tccaagggtt ctctagcaca cggtgaatat actaagggta ccgttgatat tgcaaagagt
     6961 gataaagatt tcgttattgg gttcattgct cagaacgata tgggaggaag agaagaaggg
     7021 tttgattggc taatcatgac cccaggtgta ggtttagacg acaaaggcga tgcattgggt
     7081 cagcagtaca gaaccgtcga cgaagttgta agtggtggat cagatatcat cattgttggc
     7141 agaggacttt tcgccaaggg tagagatcct aaggttgaag gtgaaagata cagaaatgct
     7201 ggatgggaag cgtaccaaaa gagaatcagc gctccccatt aattatacag gaaacttaat
     7261 agaacaaatc acatatttaa tctaatagcc acctgcattg gcacggtgca acactacttc
     7321 aacttcatct tacaaaaaga tcacgtgatc tgttgtattg ggatcgtgcc tattgatgat
     7381 ctggcggaat gtctgccggc atagccatgc cttcacatat agttgcgcgg aacccctatt
     7441 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     7501 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     7561 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     7621 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     7681 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     7741 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     7801 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     7861 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     7921 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     7981 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     8041 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     8101 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     8161 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     8221 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     8281 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     8341 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     8401 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     8461 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     8521 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     8581 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     8641 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     8701 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     8761 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     8821 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     8881 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     8941 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     9001 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     9061 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     9121 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     9181 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     9241 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     9301 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcaag
     9361 ggccatgacc acctgatgca ccaattaggt aggtctggct atgtctatac ctctggcgac
     9421 ggatcgcttg cctgtaactt acacgcgcct cgtatctttt aatgatggaa taatttggga
     9481 atttactctg tgtttattta tttttatgtt ttgtatttgg attttagaaa gtaaataaag
     9541 aaggtagaag agttacggaa tgaagaaaaa aaaataaaca aaggtttaaa aaatttcaac
     9601 aaaaagcgta ctttacatat atatttatta gacaagaaaa gcagattaaa tagatataca
     9661 ttcgattaac gataagtaaa atgtaaaatc acaggatttt cgtgtgtggt cttctacaca
     9721 gacaagatga aacaattcgg cattaatacc tgagagcagg aagagcaaga taaaaggtag
     9781 tatttgttgg cgatccccct agagtctttt acatcttcgg aaaacaaaaa ctattttttc
     9841 tttaatttct ttttttactt tctattttta atttatatat ttatattaaa aaatttaaat
     9901 tataattatt tttatagcac gtgatgaaaa ggacccaggt ggcactgccg aactttccct
     9961 gtatgaagcg atctgaccaa tcctttgccg tagtttcaac gtatg
//
