LOCUS       40924_45273        5095 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Bacterial expression vector pUChTNFR75f, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5095)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5095)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5095)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5095)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5095
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(403..1512)
                     /label=3UTR
                     /note="3UTR"
     CDS             complement(1513..2803)
                     /codon_start=1
                     /note="unnamed protein product; TNFRf"
                     /protein_id="SJL88754.1"
                     /translation="RPGAREHMPAQRIL*PDSSDVLQQMLAGPTCKSLLYQDLGHRV*L
                     L*GQHIHPALELGSRVLELWLPL*L*PGGNSSLHSGTEPHLHLQARLVLRAEQAGGVPA
                     VRAAAQVPPGLRRGQTRN*NIRRGVQALCPGDVLQHDFIHGYLQAPPDL*RGGHPWECK
                     HGCSLHVHVPHPEYGPRGSTLTPASVHTIPTHAANSRTQHCSKHLLPAPNGPQPPS*RE
                     HWRLRSSSWTDCGCDSLGSTNNRSGELCHHDPGEKEALVPAERSQGASLACR*GPGYTG
                     PRAAAPADHSAELQQQLPGELGQCVGQKGAHSEPATGTRRGGQWGRGGPGQHRELRFFP
                     WWPWDPGQCHLHRERL*QL*PQLTVLLPSQLHNGRHRFQPLGVPEGRAGPLLQGGMCLS
                     VTAGDARDPAGEHRREAPAPWSA*CWDEAQL"
     misc_feature    2805..2861
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2874..2890)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2898..2914)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2922..2952)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2967..2988)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3276..3864)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4038..4895)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4896..5000)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cnggatgtgg ctggcagagt
      421 ttggctttgt cgttggcttg ttggcctgag tggtacttac ttttttaaaa aattggcaga
      481 tttctagtta gaagtgcgtt atcttgccca ggccacagca ctgaatatgg tggcctgcct
      541 gggccctctg ggcatctgac tttcacacca tggtacaaaa ggacaagtta gcatttggag
      601 gcggtgcttt ttttttttct tttgagacag actctcgctc tgtcgcccag gctggagtgc
      661 agtggcgtga tctcggctcc ctgcaacctc cgcttcccgg gttcaaacga tttcccagcc
      721 tcaggcttct gagtagctgg gactataggt gcccgccacc acgcccggct aatttctgta
      781 tttttagtag agatggggtt ttaccatgtt ggccaggctg gtctcgaact cctaacctca
      841 ggtgatccac ccgcctcagc ctcccaaagt gctgggatca taggcgtgag ccactgcacc
      901 tggcctgagg tgatgctttc caagcctcct gagctaactt gaaggacccc gttccctaca
      961 gggctgccac ctcctccctg cacagagccc caggaccatc ccgcagtctc agcctcaggc
     1021 tgaagcactg tcctgtcttc atgggtgact caggcagcat ccctcctctc cagaaagccc
     1081 ctctgcagaa aaggatgctg gggttttctg gaagccagag ctggggcaga gcccaggggg
     1141 agaaacaaac aaacaaacaa acaaacaaac aaaaaaccca agggctccag aagccaggct
     1201 ggcaggtgca gctgggcggg gcaggtcaca gagagtcagg gacttgcccc agcatgcccc
     1261 gaacgtccat gcccagccag ccttccgaga gggacacgcc atggcagcag aggctttcca
     1321 caactcgctg cctctgctct tggcctgcag gtcagaggga ggctgcggct gtggagggca
     1381 ctagaggaac ttggcccaga aagagcctca gagtcctagt ggtgggggcc tggaaggacc
     1441 agggcccctt cgcagggtca tcctgccagg gctcagccca ccttggctac gacacagccc
     1501 acaccggcct ggttaactgg gcttcatccc agcatcaggc actccaaggg gcaggggctt
     1561 ctcttcggtg ctccccagca gggtctctgg cgtctccagc tgtgaccgaa aggcacattc
     1621 ctccttggag aaggggacct gctcgtcctt cggggactcc gaggggctgg aatctgtgtc
     1681 tcccattgtg gagctggctt gggaggagca ctgtgagctg tggtcagagc tgctacagac
     1741 gttcacgatg caggtgacat tgacctgggt cccatggcca ccaggggaag aatctgagct
     1801 cccggtgctg gcccgggcct ccccggcccc actggcctcc acgcctggtg cctgtggctg
     1861 gttccgagtg ggcgcccttc tgtccaacgc actggccgag ctctccaggg agctgctgct
     1921 ggagctcggc gctgtgatca gcaggtgctg ctgctcgggg ccctgtgtac cccgggcctt
     1981 atcggcaggc aagtgaggca ccttggcttc tctctgcagg cacaagggct tctttttcac
     2041 ctgggtcatg atgacacagt tcaccactcc tattattagt agacccaagg ctgtcacacc
     2101 cacaatcagt ccaactggaa gagcgaagtc gccagtgctc ccttcagctg gggggctggg
     2161 gcccattggg agcaggaagg aggtgcttgg agcagtgctg ggttctggag ttggctgcgt
     2221 gtgttgggat cgtgtggaca ctggctgggg taagtgtact gcccctgggg ccatactccg
     2281 ggtgggggac gtggacgtgc agactgcatc catgcttgca ttcccaggga tggccaccac
     2341 gttacagatc tggtggggcc tgcaaatatc cgtggatgaa gtcgtgttgg agaacgtccc
     2401 cggggcacag ggcttgcaca ccacgtctga tgtttcagtt cctggtctgg ccacgccgaa
     2461 gcccgggcgg cacttgcgca gcggcgcgca cagccggcac ccctcctgct tgctcagcgc
     2521 gcagtaccag ccgggcctgc aggtgcagat gcggttctgt tcccgagtgc aggcttgagt
     2581 ttccacctgg tcagagctac agcgggagcc acagctcaag cactcgggaa cccagttcca
     2641 gagctgggtg tatgtgctgt cctcacagga gtcacacacg gtgtccgagg tcttggtaca
     2701 gaagactttt gcatgttggc ccggcgagca tttgctgcag cacatctgag ctgtctggtc
     2761 atagtattct ctgagccggc atgtgctccc gggctccggg gcgngaattc gagctcggta
     2821 cccggggatc ctctagagtc gacctgcagg catgcaagct tggcgtaatc atggtcatag
     2881 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     2941 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     3001 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     3061 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     3121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     3181 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     3241 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     3301 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     3361 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     3421 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     3481 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     3541 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     3601 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     3661 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     3721 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     3781 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     3841 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     3901 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     3961 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     4021 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     4081 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     4141 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     4201 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     4261 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     4321 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     4381 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     4441 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     4501 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     4561 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     4621 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     4681 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     4741 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     4801 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     4861 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     4921 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     4981 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     5041 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtc
//
