LOCUS       40924_45268        4791 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Bacterial expression vector pUChTNFR55, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 4791)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4791)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    405..581
                     /label=5' UTR of human TNFR55
                     /note="5' UTR of human TNFR55"
     CDS             582..1946
                     /gene="TNFRSF1A"
                     /label=TNFRSF1A
                     /note="Tumor necrosis factor receptor superfamily member 1A
                     from Homo sapiens. Accession#: P19438"
     misc_feature    1950..2490
                     /label=3' UTR of human TNFR55
                     /note="3' UTR of human TNFR55"
     misc_feature    2501..2557
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2570..2586)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2594..2610)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2618..2648)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2663..2684)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2972..3560)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3734..4591)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4592..4696)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgggggggtt caagatcact
      421 gggaccaggc cgtgatctct atgcccgagt ctcaaccctc aactgtcacc ccaaggcact
      481 tgggacgtcc tggacagacc gagtcccggg aagccccagc actgccgctg ccacactgcc
      541 ctgagcccaa atgggggagt gagaggccat agctgtctgg catgggcctc tccaccgtgc
      601 ctgacctgct gctgccgctg gtgctcctgg agctgttggt gggaatatac ccctcagggg
      661 ttattggact ggtccctcac ctaggggaca gggagaagag agatagtgtg tgtccccaag
      721 gaaaatatat ccaccctcaa aataattcga tttgctgtac caagtgccac aaaggaacct
      781 acttgtacaa tgactgtcca ggcccggggc aggatacgga ctgcagggag tgtgagagcg
      841 gctccttcac cgcttcagaa aaccacctca gacactgcct cagctgctcc aaatgccgaa
      901 aggaaatggg tcaggtggag atctcttctt gcacagtgga ccgggacacc gtgtgtggct
      961 gcaggaagaa ccagtaccgg cattattgga gtgaaaacct tttccagtgc ttcaattgca
     1021 gcctctgcct caatgggacc gtgcacctct cctgccagga gaaacagaac accgtgtgca
     1081 cctgccatgc aggtttcttt ctaagagaaa acgagtgtgt ctcctgtagt aactgtaaga
     1141 aaagcctgga gtgcacgaag ttgtgcctac cccagattga gaatgttaag ggcactgagg
     1201 actcaggcac cacagtgctg ttgcccctgg tcattttctt tggtctttgc cttttatccc
     1261 tcctcttcat tggtttaatg tatcgctacc aacggtggaa gtccaagctc tactccattg
     1321 tttgtgggaa atcgacacct gaaaaagagg gggagcttga aggaactact actaagcccc
     1381 tggccccaaa cccaagcttc agtcccactc caggcttcac ccccaccctg ggcttcagtc
     1441 ccgtgcccag ttccaccttc acctccagct ccacctatac ccccggtgac tgtcccaact
     1501 ttgcggctcc ccgcagagag gtggcaccac cctatcaggg ggctgacccc atccttgcga
     1561 cagccctcgc ctccgacccc atccccaacc cccttcagaa gtgggaggac agcgcccaca
     1621 agccacagag cctagacact gatgaccccg cgacgctgta cgccgtggtg gagaacgtgc
     1681 ccccgttgcg ctggaaggaa ttcgtgcggc gcctagggct gagcgaccac gagatcgatc
     1741 ggctggagct gcagaacggg cgctgcctgc gcgaggcgca atacagcatg ctggcgacct
     1801 ggaggcggcg cacgccgcgg cgcgaggcca cgctggagct gctgggacgc gtgctccgcg
     1861 acatggacct gctgggctgc ctggaggaca tcgaggaggc gctttgcggc cccgccgccc
     1921 tcccgcccgc gcccagtctt ctcagatgag gctgcgcccc tgcgggcagc tctaaggacc
     1981 gtcctgcgag atcgccttcc aaccccactt ttttctggaa aggaggggtc ctgcaggggc
     2041 aagcaggagc tagcagccgc ctacttggtg ctaacccctc gatgtacata gcttttctca
     2101 gctgcctgcg cgccgccgac agtcagcgct gtgcgcgcgg agagaggtgc gccgtgggct
     2161 caagagcctg agtgggtggt ttgcgaggat gagggacgct atgcctcatg cccgttttgg
     2221 gtgtcctcac cagcaaggct gctcgggggc ccctggttcg tccctgagcc tttttcacag
     2281 tgcataagca gttttttttg tttttgtttt gttttgtttt gtttttaaat caatcatgtt
     2341 acactaatag aaacttggca ctcctgtgcc ctctgcctgg acaagcacat agcaagctga
     2401 actgtcctaa ggcaggggcg agcacggaac aatggggcct tcagctggag ctgtggactt
     2461 ttgtacatac actaaaattc tgaagttaaa aaaaaaaccc gaattcgagc tcggtacccg
     2521 gggatcctct agagtcgacc tgcaggcatg caagcttggc gtaatcatgg tcatagctgt
     2581 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     2641 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     2701 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     2761 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     2821 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     2881 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     2941 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     3001 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     3061 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     3121 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     3181 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     3241 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     3301 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     3361 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     3421 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     3481 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     3541 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     3601 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     3661 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     3721 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     3781 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     3841 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     3901 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     3961 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     4021 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     4081 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     4141 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     4201 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     4261 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     4321 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     4381 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     4441 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     4501 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     4561 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     4621 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     4681 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     4741 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt c
//
