LOCUS       62056_22365        3247 bp DNA     circular SYN 30-NOV-2019
DEFINITION  Cloning vector pUCTABciVI, complete sequence.
ACCESSION   MK318074
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Ghosh U, Basu U.
  TITLE     A pUC19 DERIVED PLASMID THAT GENERATES VECTOR FOR TA CLONING UPON 
            BciVI DIGESTION
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Ghosh U, Basu U.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2018) Moleculer Biology and Biotechnology, 
            University of Kalyani, Kalyani, Kalyani, West Bengal 741235, India
REFERENCE   3  (bases 1 to 3247)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-DEC-2018) Moleculer Biology and Biotechnology, University of 
            Kalyani, Kalyani, Kalyani, West Bengal 741235, India"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    287..292
                     /label=NcoI restriction site
                     /note="NcoI restriction site"
     primer_bind     complement(605..621)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(629..645)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(653..683)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(698..719)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1007..1594)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1768..2625)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2626..2730)
                     /label=AmpR promoter
     misc_feature    3060..3065
                     /label=NarI restriction site
                     /note="NarI restriction site"
     primer_bind     3204..3220
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3221..3226
                     /label=EcoRI restriction site
                     /note="EcoRI restriction site"
     misc_feature    3227..3232
                     /label=SacI restriction site
                     /note="SacI restriction site"
     misc_feature    3242..3247
                     /label=BamHI restriction site
                     /note="BamHI restriction site"
ORIGIN
        1 tgcctactcc acatatgtaa aatattttga ttcaaatata tgaacttagg aatttggcca
       61 tattatagaa acaaatacat agaatctatt tgtttgtatg gctgataaat ttataaataa
      121 ccaaaacata atggtaaaac agtcaaataa taagtgaagt tattgtacaa ggttaatgtg
      181 aacagcagaa ggaatgtaat aatgtaaagt ggtctaattc ttatgcaatt ttttctcttg
      241 gttattaaaa agacagtttc atattagaaa gcccccaccc actttcccat ggctgtatat
      301 attatacaaa tcagtgacaa atgctttacc acctccaaat acttctctgt tcattaattt
      361 acagttgcat agaaagggga gaagaaaaca ataatatacg ttattagaaa accaaaatat
      421 ccttttctct ttggatagtc agtgtttggt tccctcagta gaacacccag taagcactca
      481 acagcttgtg tcgtggagcc acggagcttt ggggttgaat gggtcttggg attggcattt
      541 gcagaagagg gcaatactga aggtcgggaa atgttggcag gcatggatac ttggcgtaat
      601 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
      661 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
      721 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
      781 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
      841 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
      901 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
      961 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     1021 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     1081 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     1141 ccctgcgctt accagatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     1201 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     1261 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     1321 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     1381 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     1441 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     1501 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     1561 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     1621 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     1681 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     1741 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     1801 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     1861 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     1921 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     1981 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     2041 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     2101 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     2161 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     2221 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     2281 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     2341 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     2401 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     2461 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     2521 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     2581 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     2641 atattattga agcatttatc agggttattg tctcatgagc ggatgcatat ttgaatgtat
     2701 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     2761 ctaagaaacc attattatca ttacattaac ctataaaaat aggcgtatca cgaggccctt
     2821 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     2881 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     2941 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
     3001 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     3061 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     3121 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     3181 agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattcgagc tcggtatccg
     3241 gggatcc
//
