LOCUS       62056_22360        9272 bp DNA     circular SYN 06-APR-2019
DEFINITION  Transformation vector pUCSW, complete sequence.
ACCESSION   MK424953
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9272)
  AUTHORS   Luo J, Shen P, Chen J.
  TITLE     A modular toolset of phiC31-based fluorescent protein tagging 
            vectors for Drosophila
  JOURNAL   Fly (Austin) (2019) In press
  PUBMED    30885036
REFERENCE   2  (bases 1 to 9272)
  AUTHORS   Luo J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2019) Model Animal Research Center, Nanjing 
            University, 12 Xu-fu Rd Pu-kou Dist, Nanjing, Jiangsu 210061, China
REFERENCE   3  (bases 1 to 9272)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fly 
            (Austin) (2019) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-JAN-2019) Model Animal Research Center, Nanjing University, 12 
            Xu-fu Rd Pu-kou Dist, Nanjing, Jiangsu 210061, China"
FEATURES             Location/Qualifiers
     source          1..9272
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            666..4802
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."
     protein_bind    4809..4842
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4843..5273
                     /label=gypsy insulator
                     /note="chromatin insulator from Drosophila"
     polyA_signal    complement(5284..5418)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(5690..5710)
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     intron          complement(5840..5905)
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     misc_feature    complement(5983..6028)
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    complement(6029..6183)
                     /label=Drosophila synthetic core promoter
                     /note="Drosophila synthetic core promoter"
     protein_bind    complement(6197..6291)
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     misc_feature    6327..6757
                     /label=gypsy insulator
                     /note="gypsy insulator"
     protein_bind    6796..6865
                     /label=attB
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     promoter        complement(7084..7102)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7123..7139)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(7147..7163)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7171..7201)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7216..7237)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7525..8113)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8287..9144)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9145..9249)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccag gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggcactaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcatt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agcattacat tacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgctattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatttggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagtta gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaaaat aaataaattt catttaactc gcgaacatgt tgaagatatg
     3181 aatattaatg agatgcgagt aacattttaa tttgcagatg gttgccatct tgattggcct
     3241 catctttttg ggccaacaac tcacgcaagt gggcgtgatg aatatcaacg gagccatctt
     3301 cctcttcctg accaacatga cctttcaaaa cgtctttgcc acgataaatg taagtcttgt
     3361 ttagaataca tttgcatatt aataatttac taactttcta atgaatcgat tcgatttagg
     3421 tgttcacctc agagctgcca gtttttatga gggaggcccg aagtcgactt tatcgctgtg
     3481 acacatactt tctgggcaaa acgattgccg aattaccgct ttttctcaca gtgccactgg
     3541 tcttcacggc gattgcctat ccgatgatcg gactgcgggc cggagtgctg cacttcttca
     3601 actgcctggc gctggtcact ctggtggcca atgtgtcaac gtccttcgga tatctaatat
     3661 cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt atcataccat
     3721 tcctgctctt tggcggcttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt
     3781 tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg
     3841 acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca
     3901 aggtcatcct ggagacgctt aacttctccg ccgccgatct gccgctggac tacgtgggtc
     3961 tggccattct catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc
     4021 gacgcaagga gtagccgaca tatatccgaa ataactgctt gttttttttt tttaccatta
     4081 ttaccatcgt gtttactgtt tattgccccc tcaaaaagct aatgtaatta tatttgtgcc
     4141 aataaaaaca agatatgacc tatagaatac aagtatttcc ccttcgaaca tccccacaag
     4201 tagactttgg atttgtcttc taaccaaaag acttacacac ctgcatacct tacatcaaaa
     4261 actcgtttat cgctacataa aacaccggga tatatttttt atatacatac ttttcaaatc
     4321 gcgcgccctc ttcataattc acctccacca caccacgttt cgtagttgct ctttcgctgt
     4381 ctcccacccg ctctccgcaa cacattcacc ttttgttcga cgaccttgga gcgactgtcg
     4441 ttagttccgc gcgattcggt tcgctcaaat ggttccgagt ggttcatttc gtctcaatag
     4501 aaattagtaa taaatatttg tatgtacaat ttatttgctc caatatattt gtatatattt
     4561 ccctcacagc tatatttatt ctaatttaat attatgactt tttaaggtaa ttttttgtga
     4621 cctgttcgga gtgattagcg ttacaatttg aactgaaagt gacatccagt gtttgttcct
     4681 tgtgtagatg catctcaaaa aaatggtggg cataatagtg ttgtttatat atatcaaaaa
     4741 taacaactat aataataaga atacatttaa tttagaaaat gcttggattt cactggaact
     4801 aggctagtat aacttcgtat aatgtatgct atacgaagtt attggccacg taataagtgt
     4861 gcgttgaatt tattcgcaaa aacattgcat attttcggca aagtaaaatt ttgttgcata
     4921 ccttatcaaa aaataagtgc tgcatacttt ttagagaaac caaataattt tttattgcat
     4981 acccgttttt aataaaatac attgcatacc ctcttttaat aaaaaatatt gcatactttg
     5041 acgaaacaaa ttttcgttgc atacccaata aaagattatt atattgcata cccgttttta
     5101 ataaaataca ttgcataccc tcttttaata aagaatattg catacgttga cgaaacaaat
     5161 tttcgttgca tacccaataa aagattatta tattgcatac cttttcttgc cataccattt
     5221 agccgatcaa ttgtgctcgg caacagtata tttgtggtgt gccaaccaac aacacgcgta
     5281 ctggatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     5341 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     5401 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     5461 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
     5521 gatctctagt caaggcacta tacatcaaat atttttccat aattttcttg tatagcagtg
     5581 cagctttttc ctttgtggtg taaatagcaa agcaagcaag agttctatta ctaaacacag
     5641 catgactcaa aaaacttagc aattctgaag gaaagtcctt ggggtcttct acctttctct
     5701 tcttttttgg aggagtagaa tgttgagagt cagcagtagc ctcatcatca ctagatggca
     5761 tttcttctga gcaaaacagg ttttcctcat taaaggcatt ccaccactgc tcccattcat
     5821 cagttccata ggttggaatc taaaatacac aaacaattag aatcagtagt ttaacacatt
     5881 atacacttaa aaattttata tttaccttag agctttaaat ctctgtaggt agtttgtcca
     5941 attatgtcac accacagaag taaggttcct tcacaaagat cctctagagg taccctcgag
     6001 ccgcggccgc agatctgtta acgaattcgt ttggtatgcg tcttgtgatt caaagttggc
     6061 ttattcaaag gatattaacg aaggcagcgg cacgtctgct cggctcgaac attcattcac
     6121 aactgatgct ctcagccacc ccgcgccctt ttataccgct gcgctcgatc cccgggcgag
     6181 ctcgctagag tctccgctcg gaggacagta ctccgctcgg aggacagtac tccgctcgga
     6241 ggacagtact ccgctcggag gacagtactc cgctcggagg acagtactcc gacctgcagg
     6301 catgcaagct tggatcctca aggccttggc cacgtaataa gtgtgcgttg aatttattcg
     6361 caaaaacatt gcatattttc ggcaaagtaa aattttgttg cataccttat caaaaaataa
     6421 gtgctgcata ctttttagag aaaccaaata attttttatt gcatacccgt ttttaataaa
     6481 atacattgca taccctcttt taataaaaaa tattgcatac tttgacgaaa caaattttcg
     6541 ttgcataccc aataaaagat tattatattg catacccgtt tttaataaaa tacattgcat
     6601 accctctttt aataaagaat attgcatacg ttgacgaaac aaattttcgt tgcataccca
     6661 ataaaagatt attatattgc ataccttttc ttgccatacc atttagccga tcaattgtgc
     6721 tcggcaacag tatatttgtg gtgtgccaac caacaacact agagatccac tagtgtcgac
     6781 gatgtaggtc acggtctcga agccgcggtg cgggtgccag ggcgtgccct tgggctcccc
     6841 gggcgcgtac tccacctcac ccatctggtc catcatgatg aacgggtcga ggtggcggta
     6901 gttgatcccg gcgaacgcgc ggcgcaccgg gaagccctcg ccctcgaaac cgctgggcgc
     6961 ggtggtcacg gtgagcacgg gacgtgcgac ggcgtcggcg ggtgcggata cgcggggcag
     7021 cgtcagcggg ttctcgacgg tcacggcggg catgtcgaca ctagttctag ccagcttttg
     7081 ttccctttag tgagggttaa tttcgagctt ggcgtaatca tggtcatagc tgtttcctgt
     7141 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     7201 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     7261 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     7321 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     7381 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     7441 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     7501 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     7561 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     7621 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     7681 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     7741 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     7801 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     7861 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     7921 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     7981 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     8041 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     8101 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     8161 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     8221 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     8281 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     8341 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     8401 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     8461 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     8521 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     8581 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     8641 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     8701 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     8761 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     8821 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     8881 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     8941 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     9001 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     9061 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     9121 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     9181 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     9241 ggttccgcgc acatttcccc gaaaagtgcc ac
//
