LOCUS       40924_45393        6384 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUCPRV_BSD2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6384)
  AUTHORS   Conlan B, Birch R, Kelso C, Holland S, De Souza AP, Long SP, Beck 
            JL, Whitney SM.
  TITLE     BSD2 is a Rubisco specific assembly chaperone, forms intermediary 
            hetero-oligomeric complexes and is non-limiting to growth in tobacco
  JOURNAL   Plant Cell Environ. (2018) In press
  PUBMED    30375663
REFERENCE   2  (bases 1 to 6384)
  AUTHORS   Conlan B, Birch R, Holland S, Kelso C, De Souza A, Long S, Beck J, 
            Whitney S.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2018) Research School of Biology, Australian 
            National University, 134 Linnaeus Way, Acton, ACT 2601, Australia
REFERENCE   3  (bases 1 to 6384)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6384)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Environ. (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-OCT-2018) Research School of Biology, Australian National 
            University, 134 Linnaeus Way, Acton, ACT 2601, Australia"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
            Nicotiana tabacum chloroplast transformation vector.
FEATURES             Location/Qualifiers
     source          1..6384
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    7..1165
                     /label=Nicotiana tabacum chloroplast flanking sequence
                     /note="Nicotiana tabacum chloroplast flanking sequence"
     regulatory      1181..1396
                     /regulatory_class="promoter"
     CDS             1399..1809
                     /codon_start=1
                     /product="BSD2"
                     /label=BSD2
                     /protein_id="AYR18863.1"
                     /translation="MANSLCFTPLASFNSVNKPGLNLINRNCTGGKIQWIKDATYSSKT
                     NLRVVEVKATDSDKDTKVRSIVCQNCDGNGAVSCSQCKGTGVNSVDHFNGRFKAGGLCW
                     LCRGKKDMLCGDCNGAGFLGGFMSTFDEHHHH"
     CDS             1859..2647
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     misc_feature    2810..3725
                     /label=Nicotiana tabacum chloroplast flanking sequence
                     /note="Nicotiana tabacum chloroplast flanking sequence"
     primer_bind     complement(3768..3784)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3792..3808)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3816..3846)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3861..3882)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4170..4758)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4932..5789)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5790..5894)
                     /label=AmpR promoter
     primer_bind     6368..6384
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattcttga tcaatccctt tgcccctcat tcttcaagaa taaggaagat ccttttcaag
       61 tttgaatttg ttcatttgga atctgggttc ttctacttca tatttattta atatgaatat
      121 tttccctctc ttttttttat atcattcctt aagtcccata ggtttgatcc tgtagaattt
      181 gacccatttt ctcattgaac gaaaggtacg aaataaatca gattgataaa agtaccatgt
      241 gaaatcttcg gtttttcccc ttcctcgatc cctatcccat aggttaggta cagtgtttga
      301 atcaatagag aaccttttct tctgtatgaa tcgatattat tccattccaa atccttcccg
      361 atacctccca aggaaaatct cgaatttgga ttccaaattg acgggttagt gtgagcttat
      421 ccatgcggtt atgcactctt tgaataggaa tccgttttct gaaagatcct ggctttcgta
      481 ctttggtggg tctccgagat cctttcgatg acctatgttg aagggatatc tatctaatcc
      541 gatcgattgc gtaaagcccg cggtagcaac ggaaccgggg aaagtataca gaaaagacag
      601 ttcttttcta ttatattagt attttctatt atattagata tattagacta ttatattaga
      661 ttagtattag ttagtgatcc cgacttagtg agtctgatga attgttggca ccagtcctac
      721 attttgtctc tgtggaccga ggagaaaagg ggctcggcgg gaagaggagt gtaccatgag
      781 agaagcaagg aggtcaacct ctttcaaata tacaacatgg attctggcaa tgtagttgga
      841 ctctcatgtc gatccgaatg aatcatcctt tccacggagg taaatctttg cctgctaggc
      901 aagaggatag caagttccaa attctgtctc ggtaggacat gtatttctat tactatgaaa
      961 ttcataaatg aagtagttaa tggtagggtt accattatcc tttttgtagt gacgaatctt
     1021 gtatgtgttc ctaagaaaag gaatttgtcc atttttcggg gtctcaaagg ggcgtggaaa
     1081 cgcataagaa ctcttgaatg gaaaagagat gtaactccag ttccttcgga atcggtagtc
     1141 aatcctattt ccgatagggg cagttgcggc cgcgagatct cccactagca tatcgaaatt
     1201 ctaattttct gtagagaagt ccgtattttt ccaatcaact tcattaaaaa tttgaataga
     1261 tcaacataca ccttggttga cacgagtata taagtcatgt tatactgttg aataacaatc
     1321 cttccatttt ctattttgat ttgtagaaaa ctagtgtgct tgggagtccc tgatgattaa
     1381 ataaaccaag attttaccat ggcaaactca ctttgcttca cacctttggc tagcttcaat
     1441 tctgttaata aaccaggtct aaatctgatt aatagaaatt gcaccggagg aaagattcaa
     1501 tggatcaaag atgctaccta cagctccaag actaacttga gagttgtaga agttaaggcc
     1561 actgacagtg acaaagacac gaaagtaaga agcattgtat gtcaaaattg tgatggaaat
     1621 ggtgcagtgt cttgctcaca atgcaaaggc acaggggtta actctgtaga tcatttcaat
     1681 ggacgattca aagctggtgg actgtgttgg ctctgcagag gtaaaaaaga tatgttatgt
     1741 ggtgattgca atggtgctgg ttttcttggt ggatttatgt ctacttttga tgaacatcac
     1801 catcactaaa aacagtcgag ctcagataaa tacttttaaa aattaaaggg agggatttat
     1861 gtcacctgcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga
     1921 gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg
     1981 cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac
     2041 aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga
     2101 gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta
     2161 tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat
     2221 cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca
     2281 tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc ttgaacagga
     2341 tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg
     2401 cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa
     2461 aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca
     2521 gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc
     2581 tcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt
     2641 cggcaaataa tgtctagcta gagaaattca attaaggaaa taaattaagg aaatacaaaa
     2701 aggggggtag tcatttgtat ataactttgt atgacttttc tcttctattt ttttgtattt
     2761 cctccctttc cttttctatt tgtatttttt tatcattgct tccaagctta attgaatccg
     2821 attttgacca ttattttcat atccgtaata gtgcgaaaag aaggcccggc tccaagttgt
     2881 tcaagaatag tggcgttgag tttctcgacc ctttgactta ggattagtca gttctatttc
     2941 tcgatggggc ggggaaggga tataactcag cggtagagtg tcaccttgac gtggtggaag
     3001 tcatcagttc gagcctgatt atccctaagc ccaatgtgag tttttctagt tggatttgct
     3061 cccccgccgt cgttcaatga gaatggataa gaggctcgtg ggattgacgt gagggggcag
     3121 ggatggctat atttctggga gcgaactccg ggcgaatatg aagcgcatgg atacaagtta
     3181 tgccttggaa tgaaagacaa ttccgaatcc gctttgtcta cgaacaagga agctataagt
     3241 aatgcaacta tgaatctcat ggagagttcg atcctggctc aggatgaacg ctggcggcat
     3301 gcttaacaca tgcaagtcgg acgggaagtg gtgtttccag tggcggacgg gtgagtaacg
     3361 cgtaagaacc tgcccttggg aggggaacaa cagctggaaa cggctgctaa taccccgtag
     3421 gctgaggagc aaaaggagga atccgcccga ggaggggctc gcgtctgatt agctagttgg
     3481 tgaggcaata gcttaccaag gcgatgatca gtagctggtc cgagaggatg atcagccaca
     3541 ctgggactga gacacggccc agactcctac gggaggcagc agtggggaat tttccgcaat
     3601 gggcgaaagc tgacggagca atgccgcgtg gaggtagaag gcccacgggt cgtgaacttc
     3661 ttttcccgga gaagaagcaa tgacggtatc tggggaataa gcatcggcta actctgtgcc
     3721 agcagccgcg gctgcagagg cctgcatgca agcttggcgt aatcatggtc atagctgttt
     3781 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     3841 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     3901 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     3961 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     4021 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     4081 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     4141 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     4201 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     4261 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     4321 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     4381 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     4441 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     4501 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     4561 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     4621 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     4681 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     4741 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     4801 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     4861 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     4921 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     4981 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     5041 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     5101 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     5161 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     5221 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     5281 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     5341 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     5401 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     5461 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     5521 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     5581 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     5641 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     5701 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     5761 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     5821 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     5881 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     5941 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
     6001 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     6061 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     6121 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     6181 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc
     6241 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     6301 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     6361 cgacgttgta aaacgacggc cagt
//
