LOCUS       40924_45378        4972 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUCP30T-mKeima, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Barbier M, Damron FH.
  TITLE     Rainbow Vectors for Broad-Range Bacterial Fluorescence Labeling
  JOURNAL   PLoS ONE 11 (3), E0146827 (2016)
  PUBMED    26937640
REFERENCE   2  (bases 1 to 4972)
  AUTHORS   Barbier M, Damron FH.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2015) Microbiology, Immunology and Cell Biology, 
            West Virginia University, School of Medicine, One Medical Center 
            Drive, Morgantown, WV 26506, USA
REFERENCE   3  (bases 1 to 4972)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4972)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2016"; volume: "11"; issue: "3"; pages: "E0146827"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-OCT-2015) Microbiology, Immunology and Cell Biology, West 
            Virginia University, School of Medicine, One Medical Center Drive, 
            Morgantown, WV 26506, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4972
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      72..660
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     oriT            complement(732..840)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     protein_bind    1224..1245
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1260..1290
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1298..1314
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1322..1338
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1369..2034
                     /codon_start=1
                     /label=mKeima-Red
                     /note="monomeric Keima-Red fluorescent protein, also known
                     as mKeima"
                     /translation="MVSVIAKQMTYKVYMSGTVNGHYFEVEGDGKGKPYEGEQTVKLTV
                     TKGGPLPFAWDILSPQLQYGSIPFTKYPEDIPDYFKQSFPEGYTWERSMNFEDGAVCTV
                     SNDSSIQGNCFIYNVKISGENFPPNGPVMQKKTQGWEPSTERLFARDGMLIGNDYMALK
                     LEGGGHYLCEFKSTYKAKKPVRMPGRHEIDRKLDVTSHNRDYTSVEQCEIAIARHSLLG
                     "
     primer_bind     complement(2077..2093)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2257..3087)
                     /codon_start=1
                     /label=pRO1600 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
                     /translation="VASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     rep_origin      3101..3452
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     promoter        3493..3521
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             3710..4240
                     /codon_start=1
                     /label=GmR
                     /note="gentamycin acetyltransferase"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
       61 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
      121 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
      181 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
      241 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
      301 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
      361 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
      421 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
      481 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
      541 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
      601 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
      661 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacata
      721 agctagctta tcggccagcc tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa
      781 taagggacag tgaagaagga acacccgctc gcgggtgggc ctacttcacc tatcctgccc
      841 ggctgacgcc gttggataca ccaaggaaag tctacacgaa ccctttggca aaatcctgta
      901 tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta taatgacccc gaagcagggt
      961 tatgcagcgg aaagtatacc ttaaggaatc cccatgttct ttcctgcgtt atcccctgat
     1021 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     1081 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     1141 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     1201 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     1261 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     1321 acaggaaaca gctatgacca tgattacgaa ttcggcgcgc caagaaggat ggtttctgtg
     1381 atcgctaaac aaatgactta taaagtctac atgagtggaa cagtaaacgg ccattacttc
     1441 gaggtagaag gtgatggtaa aggaaaacca tacgaaggcg aacaaaccgt gaaattgact
     1501 gttaccaaag gaggaccact cccatttgcc tgggatatcc tttcacctca attgcaatac
     1561 ggtagtatac cattcacaaa atatcctgaa gatattccag actactttaa acaatctttc
     1621 ccagaaggat atacatggga gagatccatg aatttcgaag atggtgctgt ttgtacggtg
     1681 tcaaatgact cttctataca gggcaattgt tttatctaca acgttaagat ttcaggcgag
     1741 aattttcctc caaatggtcc agtaatgcaa aagaaaacac aggggtggga accatctact
     1801 gaaaggttat ttgccagaga tggtatgctt attggtaacg actacatggc actaaagctg
     1861 gaagggggtg gtcattactt atgtgagttt aagtcaactt ataaggcgaa aaagcctgtc
     1921 agaatgcctg gcagacacga gatcgaccgt aagttggatg ttactagcca caacagagat
     1981 tacacatccg tcgaacaatg cgaaattgct atcgcaagac attctctatt agggtaagga
     2041 tcctctagag tcgacctgca ggcatgcaag cttggcactg gccgtcgttt tacaacgtcg
     2101 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     2161 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     2221 gaaaggcagg ccgggccgtg gtggccacgg cctctaggcc agatccagcg gcatctgggt
     2281 tagtcgagcg cgggccgctt cccatgtctc accagggcga gcctgtttcg cgatctcagc
     2341 atctgaaatc ttcccggcct tgcgcttcgc tggggcctta cccaccgcct tggcgggctt
     2401 cttcggtcca aaactgaaca acagatgtgt gaccttgcgc ccggtctttc gctgcgccca
     2461 ctccacctgt agcgggctgt gctcgttgat ctgcgtcacg gctggatcaa gcactcgcaa
     2521 cttgaagtcc ttgatcgagg gataccggcc ttccagttga aaccactttc gcagctggtc
     2581 aatttctatt tcgcgctggc cgatgctgtc ccattgcatg agcagctcgt aaagcctgat
     2641 cgcgtgggtg ctgtccatct tggccacgtc agccaaggcg tatttggtga actgtttggt
     2701 gagttccgtc aggtacggca gcatgtcttt ggtgaacctg agttctacac ggccctcacc
     2761 ctcccggtag atgattgttt gcacccagcc ggtaatcatc acactcggtc ttttcccctt
     2821 gccattgggc tcttgggtta accggacttc ccgccgtttc aggcgcaggg ccgcttcttt
     2881 gagctggttg taggaagatt cgatagggac acccgccatc gtcgctatgt cctccgccgt
     2941 cactgaatac atcacttcat cggtgacagg ctcgctcctc ttcacctggc taatacaggc
     3001 cagaacgatc cgctgttcct gaacactgag gcgatacgcg gcctcgacca gggcattgct
     3061 tttgtaaacc attgggggtg aggccacgtt cgacattcct tgtgtataag gggacactgt
     3121 atctgcgtcc cacaatacaa caaatccgtc cctttacaac aacaaatccg tcccttctta
     3181 acaacaaatc cgtcccttaa tggcaacaaa tccgtccctt tttaaactct acaggccacg
     3241 gattacgtgg cctgtagacg tcctaaaagg tttaaaaggg aaaaggaaga aaagggtgga
     3301 aacgcaaaaa acgcaccact acgtggcccc gttggggccg catttgtgcc cctgaagggg
     3361 cgggggaggc gtctgggcaa tccccgtttt accagtcccc tatcgccgcc tgagagggcg
     3421 caggaagcga gtaatcaggg tatcgaggcg gattcaccct tggcgtccaa ccagcggcac
     3481 cagcggcgcg aattgacata agcctgttcg gttcgtaaac tgtaatgcaa gtagcgtatg
     3541 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
     3601 gttttcatgg cttgttatga ctgttttttt gtacagtcta tgcctcgggc atccaagcag
     3661 caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag
     3721 cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaggtg gctcaagtat
     3781 gggcatcatt cgcacatgta ggctcggccc tgaccaagtc aaatccatgc gggctgctct
     3841 tgatcttttc ggtcgtgagt tcggagacgt agccacctac tcccaacatc agccggactc
     3901 cgattacctc gggaacttgc tccgtagtaa gacattcatc gcgcttgctg ccttcgacca
     3961 agaagcggtt gttggcgctc tcgcggctta cgttctgccc aggtttgagc agccgcgtag
     4021 tgagatctat atctatgatc tcgcagtctc cggcgagcac cggaggcagg gcattgccac
     4081 cgcgctcatc aatctcctca agcatgaggc caacgcgctt ggtgcttatg tgatctacgt
     4141 gcaagcagat tacggtgacg atcccgcagt ggctctctat acaaagttgg gcatacggga
     4201 agaagtgatg cactttgata tcgacccaag taccgccacc taacaattcg ttcaagccga
     4261 gatcggcttc ccggccgcgg agttgttcgg taaattgtca caacgccgcg gccaattcga
     4321 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     4381 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     4441 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     4501 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     4561 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     4621 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     4681 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     4741 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     4801 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     4861 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     4921 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tg
//
