LOCUS       40924_45368        5174 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUCP30T-eYFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5174)
  AUTHORS   Barbier M, Damron FH.
  TITLE     Rainbow Vectors for Broad-Range Bacterial Fluorescence Labeling
  JOURNAL   PLoS ONE 11 (3), E0146827 (2016)
  PUBMED    26937640
REFERENCE   2  (bases 1 to 5174)
  AUTHORS   Barbier M, Damron FH.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2015) Microbiology, Immunology and Cell Biology, 
            West Virginia University, School of Medicine, One Medical Center 
            Drive, Morgantown, WV 26506, USA
REFERENCE   3  (bases 1 to 5174)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5174)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2016"; volume: "11"; issue: "3"; pages: "E0146827"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-OCT-2015) Microbiology, Immunology and Cell Biology, West 
            Virginia University, School of Medicine, One Medical Center Drive, 
            Morgantown, WV 26506, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5174
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      72..660
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     oriT            complement(732..840)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     protein_bind    1224..1245
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1260..1290
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1298..1314
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1322..1338
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     RBS             1378..1389
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1396..2112
                     /label=EYFP
                     /note="enhanced YFP"
     terminator      2138..2232
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(2279..2295)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2459..3289)
                     /label=pRO1600 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
     rep_origin      3303..3654
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     promoter        3695..3723
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             3912..4442
                     /label=GmR
                     /note="gentamycin acetyltransferase"
ORIGIN
        1 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
       61 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
      121 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
      181 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
      241 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
      301 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
      361 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
      421 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
      481 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
      541 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
      601 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
      661 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacata
      721 agctagctta tcggccagcc tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa
      781 taagggacag tgaagaagga acacccgctc gcgggtgggc ctacttcacc tatcctgccc
      841 ggctgacgcc gttggataca ccaaggaaag tctacacgaa ccctttggca aaatcctgta
      901 tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta taatgacccc gaagcagggt
      961 tatgcagcgg aaagtatacc ttaaggaatc cccatgttct ttcctgcgtt atcccctgat
     1021 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     1081 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     1141 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     1201 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     1261 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     1321 acaggaaaca gctatgacca tgattacgaa ttcggcgcgc caagaaggtc tagaattaaa
     1381 gaggagaaat taagcatgct gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     1441 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     1501 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     1561 gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgcagtgctt cgcccgctac
     1621 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     1681 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     1741 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     1801 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     1861 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     1921 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     1981 ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag
     2041 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     2101 gagctgtaca agtaataagc ttaattagct gagcttggac tcctgttgat agatccagta
     2161 atgacctcag aactccatct ggatttgttc agaacgctcg gttgccgccg ggcgtttttt
     2221 attggtgaga atccaagctg gatcctctag agtcgacctg caggcatgca agcttggcac
     2281 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     2341 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     2401 cttcccaaca gttgcgcagc ctgaaaggca ggccgggccg tggtggccac ggcctctagg
     2461 ccagatccag cggcatctgg gttagtcgag cgcgggccgc ttcccatgtc tcaccagggc
     2521 gagcctgttt cgcgatctca gcatctgaaa tcttcccggc cttgcgcttc gctggggcct
     2581 tacccaccgc cttggcgggc ttcttcggtc caaaactgaa caacagatgt gtgaccttgc
     2641 gcccggtctt tcgctgcgcc cactccacct gtagcgggct gtgctcgttg atctgcgtca
     2701 cggctggatc aagcactcgc aacttgaagt ccttgatcga gggataccgg ccttccagtt
     2761 gaaaccactt tcgcagctgg tcaatttcta tttcgcgctg gccgatgctg tcccattgca
     2821 tgagcagctc gtaaagcctg atcgcgtggg tgctgtccat cttggccacg tcagccaagg
     2881 cgtatttggt gaactgtttg gtgagttccg tcaggtacgg cagcatgtct ttggtgaacc
     2941 tgagttctac acggccctca ccctcccggt agatgattgt ttgcacccag ccggtaatca
     3001 tcacactcgg tcttttcccc ttgccattgg gctcttgggt taaccggact tcccgccgtt
     3061 tcaggcgcag ggccgcttct ttgagctggt tgtaggaaga ttcgataggg acacccgcca
     3121 tcgtcgctat gtcctccgcc gtcactgaat acatcacttc atcggtgaca ggctcgctcc
     3181 tcttcacctg gctaatacag gccagaacga tccgctgttc ctgaacactg aggcgatacg
     3241 cggcctcgac cagggcattg cttttgtaaa ccattggggg tgaggccacg ttcgacattc
     3301 cttgtgtata aggggacact gtatctgcgt cccacaatac aacaaatccg tccctttaca
     3361 acaacaaatc cgtcccttct taacaacaaa tccgtccctt aatggcaaca aatccgtccc
     3421 tttttaaact ctacaggcca cggattacgt ggcctgtaga cgtcctaaaa ggtttaaaag
     3481 ggaaaaggaa gaaaagggtg gaaacgcaaa aaacgcacca ctacgtggcc ccgttggggc
     3541 cgcatttgtg cccctgaagg ggcgggggag gcgtctgggc aatccccgtt ttaccagtcc
     3601 cctatcgccg cctgagaggg cgcaggaagc gagtaatcag ggtatcgagg cggattcacc
     3661 cttggcgtcc aaccagcggc accagcggcg cgaattgaca taagcctgtt cggttcgtaa
     3721 actgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag
     3781 cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttgtacagtc
     3841 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg
     3901 gagcagcaac gatgttacgc agcagcaacg atgttacgca gcagggcagt cgccctaaaa
     3961 caaagttagg tggctcaagt atgggcatca ttcgcacatg taggctcggc cctgaccaag
     4021 tcaaatccat gcgggctgct cttgatcttt tcggtcgtga gttcggagac gtagccacct
     4081 actcccaaca tcagccggac tccgattacc tcgggaactt gctccgtagt aagacattca
     4141 tcgcgcttgc tgccttcgac caagaagcgg ttgttggcgc tctcgcggct tacgttctgc
     4201 ccaggtttga gcagccgcgt agtgagatct atatctatga tctcgcagtc tccggcgagc
     4261 accggaggca gggcattgcc accgcgctca tcaatctcct caagcatgag gccaacgcgc
     4321 ttggtgctta tgtgatctac gtgcaagcag attacggtga cgatcccgca gtggctctct
     4381 atacaaagtt gggcatacgg gaagaagtga tgcactttga tatcgaccca agtaccgcca
     4441 cctaacaatt cgttcaagcc gagatcggct tcccggccgc ggagttgttc ggtaaattgt
     4501 cacaacgccg cggccaattc gactcaccag tcacagaaaa gcatcttacg gatggcatga
     4561 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     4621 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     4681 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     4741 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     4801 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     4861 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     4921 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     4981 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     5041 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     5101 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     5161 ttgataatct catg
//
