LOCUS       40924_45373        5168 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUCP30T-GFPmut3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5168)
  AUTHORS   Barbier M, Damron FH.
  TITLE     Rainbow Vectors for Broad-Range Bacterial Fluorescence Labeling
  JOURNAL   PLoS ONE 11 (3), E0146827 (2016)
  PUBMED    26937640
REFERENCE   2  (bases 1 to 5168)
  AUTHORS   Barbier M, Damron FH.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2015) Microbiology, Immunology and Cell Biology, 
            West Virginia University, School of Medicine, One Medical Center 
            Drive, Morgantown, WV 26506, USA
REFERENCE   3  (bases 1 to 5168)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5168)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2016"; volume: "11"; issue: "3"; pages: "E0146827"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-OCT-2015) Microbiology, Immunology and Cell Biology, West 
            Virginia University, School of Medicine, One Medical Center Drive, 
            Morgantown, WV 26506, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5168
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      72..660
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     oriT            complement(732..840)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     protein_bind    1224..1245
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1260..1290
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1298..1314
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1322..1338
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     RBS             1378..1389
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1396..2109
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     terminator      2132..2226
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(2273..2289)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2453..3283)
                     /label=pRO1600 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
     rep_origin      3297..3648
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     promoter        3689..3717
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             3906..4436
                     /label=GmR
                     /note="gentamycin acetyltransferase"
ORIGIN
        1 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
       61 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
      121 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
      181 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
      241 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
      301 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
      361 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
      421 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
      481 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
      541 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
      601 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
      661 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacata
      721 agctagctta tcggccagcc tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa
      781 taagggacag tgaagaagga acacccgctc gcgggtgggc ctacttcacc tatcctgccc
      841 ggctgacgcc gttggataca ccaaggaaag tctacacgaa ccctttggca aaatcctgta
      901 tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta taatgacccc gaagcagggt
      961 tatgcagcgg aaagtatacc ttaaggaatc cccatgttct ttcctgcgtt atcccctgat
     1021 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     1081 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     1141 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     1201 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     1261 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     1321 acaggaaaca gctatgacca tgattacgaa ttcggcgcgc caagaaggtc tagaattaaa
     1381 gaggagaaat taagcatgcg taaaggagaa gaacttttca ctggagttgt cccaattctt
     1441 gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt
     1501 gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt
     1561 ccatggccaa cacttgtcac tactttcggt tatggtgttc aatgctttgc gagataccca
     1621 gatcatatga aacggcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa
     1681 agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa
     1741 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac
     1801 attcttggac acaaattgga atacaactat aactcacaca atgtatacat catggcagac
     1861 aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc
     1921 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta
     1981 ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga
     2041 gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa
     2101 ctatacaaat aagcttaatt agctgagctt ggactcctgt tgatagatcc agtaatgacc
     2161 tcagaactcc atctggattt gttcagaacg ctcggttgcc gccgggcgtt ttttattggt
     2221 gagaatccaa gctggatcct ctagagtcga cctgcaggca tgcaagcttg gcactggccg
     2281 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     2341 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     2401 aacagttgcg cagcctgaaa ggcaggccgg gccgtggtgg ccacggcctc taggccagat
     2461 ccagcggcat ctgggttagt cgagcgcggg ccgcttccca tgtctcacca gggcgagcct
     2521 gtttcgcgat ctcagcatct gaaatcttcc cggccttgcg cttcgctggg gccttaccca
     2581 ccgccttggc gggcttcttc ggtccaaaac tgaacaacag atgtgtgacc ttgcgcccgg
     2641 tctttcgctg cgcccactcc acctgtagcg ggctgtgctc gttgatctgc gtcacggctg
     2701 gatcaagcac tcgcaacttg aagtccttga tcgagggata ccggccttcc agttgaaacc
     2761 actttcgcag ctggtcaatt tctatttcgc gctggccgat gctgtcccat tgcatgagca
     2821 gctcgtaaag cctgatcgcg tgggtgctgt ccatcttggc cacgtcagcc aaggcgtatt
     2881 tggtgaactg tttggtgagt tccgtcaggt acggcagcat gtctttggtg aacctgagtt
     2941 ctacacggcc ctcaccctcc cggtagatga ttgtttgcac ccagccggta atcatcacac
     3001 tcggtctttt ccccttgcca ttgggctctt gggttaaccg gacttcccgc cgtttcaggc
     3061 gcagggccgc ttctttgagc tggttgtagg aagattcgat agggacaccc gccatcgtcg
     3121 ctatgtcctc cgccgtcact gaatacatca cttcatcggt gacaggctcg ctcctcttca
     3181 cctggctaat acaggccaga acgatccgct gttcctgaac actgaggcga tacgcggcct
     3241 cgaccagggc attgcttttg taaaccattg ggggtgaggc cacgttcgac attccttgtg
     3301 tataagggga cactgtatct gcgtcccaca atacaacaaa tccgtccctt tacaacaaca
     3361 aatccgtccc ttcttaacaa caaatccgtc ccttaatggc aacaaatccg tcccttttta
     3421 aactctacag gccacggatt acgtggcctg tagacgtcct aaaaggttta aaagggaaaa
     3481 ggaagaaaag ggtggaaacg caaaaaacgc accactacgt ggccccgttg gggccgcatt
     3541 tgtgcccctg aaggggcggg ggaggcgtct gggcaatccc cgttttacca gtcccctatc
     3601 gccgcctgag agggcgcagg aagcgagtaa tcagggtatc gaggcggatt cacccttggc
     3661 gtccaaccag cggcaccagc ggcgcgaatt gacataagcc tgttcggttc gtaaactgta
     3721 atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg
     3781 taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgtac agtctatgcc
     3841 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
     3901 caacgatgtt acgcagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt
     3961 taggtggctc aagtatgggc atcattcgca catgtaggct cggccctgac caagtcaaat
     4021 ccatgcgggc tgctcttgat cttttcggtc gtgagttcgg agacgtagcc acctactccc
     4081 aacatcagcc ggactccgat tacctcggga acttgctccg tagtaagaca ttcatcgcgc
     4141 ttgctgcctt cgaccaagaa gcggttgttg gcgctctcgc ggcttacgtt ctgcccaggt
     4201 ttgagcagcc gcgtagtgag atctatatct atgatctcgc agtctccggc gagcaccgga
     4261 ggcagggcat tgccaccgcg ctcatcaatc tcctcaagca tgaggccaac gcgcttggtg
     4321 cttatgtgat ctacgtgcaa gcagattacg gtgacgatcc cgcagtggct ctctatacaa
     4381 agttgggcat acgggaagaa gtgatgcact ttgatatcga cccaagtacc gccacctaac
     4441 aattcgttca agccgagatc ggcttcccgg ccgcggagtt gttcggtaaa ttgtcacaac
     4501 gccgcggcca attcgactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     4561 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     4621 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     4681 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     4741 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     4801 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     4861 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     4921 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     4981 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     5041 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     5101 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     5161 atctcatg
//
