LOCUS       40924_45363        5169 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUCP30T-E2Crimson, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5169)
  AUTHORS   Barbier M, Damron FH.
  TITLE     Rainbow Vectors for Broad-Range Bacterial Fluorescence Labeling
  JOURNAL   PLoS ONE 11 (3), E0146827 (2016)
  PUBMED    26937640
REFERENCE   2  (bases 1 to 5169)
  AUTHORS   Barbier M, Damron FH.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2015) Microbiology, Immunology and Cell Biology, 
            West Virginia University, School of Medicine, One Medical Center 
            Drive, Morgantown, WV 26506, USA
REFERENCE   3  (bases 1 to 5169)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5169)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2016"; volume: "11"; issue: "3"; pages: "E0146827"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-OCT-2015) Microbiology, Immunology and Cell Biology, West 
            Virginia University, School of Medicine, One Medical Center Drive, 
            Morgantown, WV 26506, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      72..660
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     oriT            complement(732..840)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     protein_bind    1224..1245
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1260..1290
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1298..1314
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1322..1338
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1518..2192
                     /codon_start=1
                     /label=E2-Crimson
                     /note="far-red noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2009)"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGVGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFFYGSKAYIKHPADIPDYLKQSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTLIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERNYPRDGVLKGENHM
                     ALKLKGGGHYLCEFKSIYMAKKPVKLPGYHYVDYKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(2274..2290)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2454..3284)
                     /codon_start=1
                     /label=pRO1600 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
                     /translation="VASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     rep_origin      3298..3649
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     promoter        3690..3718
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             3907..4437
                     /codon_start=1
                     /label=GmR
                     /note="gentamycin acetyltransferase"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
       61 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
      121 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
      181 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
      241 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
      301 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
      361 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
      421 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
      481 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
      541 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
      601 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
      661 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacata
      721 agctagctta tcggccagcc tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa
      781 taagggacag tgaagaagga acacccgctc gcgggtgggc ctacttcacc tatcctgccc
      841 ggctgacgcc gttggataca ccaaggaaag tctacacgaa ccctttggca aaatcctgta
      901 tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta taatgacccc gaagcagggt
      961 tatgcagcgg aaagtatacc ttaaggaatc cccatgttct ttcctgcgtt atcccctgat
     1021 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     1081 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     1141 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     1201 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     1261 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     1321 acaggaaaca gctatgacca tgattacgaa ttcggcgcgc catgtttttc aacagactaa
     1381 gcgctggcaa gctgctggta ccactctccg tggtcctgta cgcccttttc gtggtaatat
     1441 tacctttaca gaattctttc cactcctcca atgttttagt tagaggtgcc gatgatgggg
     1501 atccactagt cgccaccatg gatagcactg agaacgtcat caagcccttc atgcgcttca
     1561 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gtgggcgagg
     1621 gcaagcccta cgagggcacc cagaccgcca agctgcaagt gaccaagggc ggccccctgc
     1681 ccttcgcctg ggacatcctg tccccccagt tcttctacgg ctccaaggcg tacatcaagc
     1741 accccgccga catccccgac tacctcaagc agtccttccc cgagggcttc aagtgggagc
     1801 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
     1861 acggcaccct catctaccac gtgaagttca tcggcgtgaa cttcccctcc gacggccccg
     1921 taatgcagaa gaagactctg ggctgggagc cctccactga gcgcaactac ccccgcgacg
     1981 gcgtgctgaa gggcgagaac cacatggcgc tgaagctgaa gggcggcggc cactacctgt
     2041 gtgagttcaa gtccatctac atggccaaga agcccgtgaa gctgcccggc taccactacg
     2101 tggactacaa gctcgacatc acctcccaca acgaggacta caccgtggtg gagcagtacg
     2161 agcgcgccga ggcccgccac cacctgttcc agactcacgg tatggacgaa ttgtacaagc
     2221 acgacgaatt gtaaggatcc tctagagtcg acctgcaggc atgcaagctt ggcactggcc
     2281 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     2341 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     2401 caacagttgc gcagcctgaa aggcaggccg ggccgtggtg gccacggcct ctaggccaga
     2461 tccagcggca tctgggttag tcgagcgcgg gccgcttccc atgtctcacc agggcgagcc
     2521 tgtttcgcga tctcagcatc tgaaatcttc ccggccttgc gcttcgctgg ggccttaccc
     2581 accgccttgg cgggcttctt cggtccaaaa ctgaacaaca gatgtgtgac cttgcgcccg
     2641 gtctttcgct gcgcccactc cacctgtagc gggctgtgct cgttgatctg cgtcacggct
     2701 ggatcaagca ctcgcaactt gaagtccttg atcgagggat accggccttc cagttgaaac
     2761 cactttcgca gctggtcaat ttctatttcg cgctggccga tgctgtccca ttgcatgagc
     2821 agctcgtaaa gcctgatcgc gtgggtgctg tccatcttgg ccacgtcagc caaggcgtat
     2881 ttggtgaact gtttggtgag ttccgtcagg tacggcagca tgtctttggt gaacctgagt
     2941 tctacacggc cctcaccctc ccggtagatg attgtttgca cccagccggt aatcatcaca
     3001 ctcggtcttt tccccttgcc attgggctct tgggttaacc ggacttcccg ccgtttcagg
     3061 cgcagggccg cttctttgag ctggttgtag gaagattcga tagggacacc cgccatcgtc
     3121 gctatgtcct ccgccgtcac tgaatacatc acttcatcgg tgacaggctc gctcctcttc
     3181 acctggctaa tacaggccag aacgatccgc tgttcctgaa cactgaggcg atacgcggcc
     3241 tcgaccaggg cattgctttt gtaaaccatt gggggtgagg ccacgttcga cattccttgt
     3301 gtataagggg acactgtatc tgcgtcccac aatacaacaa atccgtccct ttacaacaac
     3361 aaatccgtcc cttcttaaca acaaatccgt cccttaatgg caacaaatcc gtcccttttt
     3421 aaactctaca ggccacggat tacgtggcct gtagacgtcc taaaaggttt aaaagggaaa
     3481 aggaagaaaa gggtggaaac gcaaaaaacg caccactacg tggccccgtt ggggccgcat
     3541 ttgtgcccct gaaggggcgg gggaggcgtc tgggcaatcc ccgttttacc agtcccctat
     3601 cgccgcctga gagggcgcag gaagcgagta atcagggtat cgaggcggat tcacccttgg
     3661 cgtccaacca gcggcaccag cggcgcgaat tgacataagc ctgttcggtt cgtaaactgt
     3721 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
     3781 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttgta cagtctatgc
     3841 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
     3901 gcaacgatgt tacgcagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag
     3961 ttaggtggct caagtatggg catcattcgc acatgtaggc tcggccctga ccaagtcaaa
     4021 tccatgcggg ctgctcttga tcttttcggt cgtgagttcg gagacgtagc cacctactcc
     4081 caacatcagc cggactccga ttacctcggg aacttgctcc gtagtaagac attcatcgcg
     4141 cttgctgcct tcgaccaaga agcggttgtt ggcgctctcg cggcttacgt tctgcccagg
     4201 tttgagcagc cgcgtagtga gatctatatc tatgatctcg cagtctccgg cgagcaccgg
     4261 aggcagggca ttgccaccgc gctcatcaat ctcctcaagc atgaggccaa cgcgcttggt
     4321 gcttatgtga tctacgtgca agcagattac ggtgacgatc ccgcagtggc tctctataca
     4381 aagttgggca tacgggaaga agtgatgcac tttgatatcg acccaagtac cgccacctaa
     4441 caattcgttc aagccgagat cggcttcccg gccgcggagt tgttcggtaa attgtcacaa
     4501 cgccgcggcc aattcgactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     4561 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     4621 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     4681 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     4741 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     4801 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     4861 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     4921 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     4981 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     5041 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     5101 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     5161 aatctcatg
//
