LOCUS       40924_45318        5039 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUCP20T-GFPmut3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Barbier M, Damron FH.
  TITLE     Rainbow Vectors for Broad-Range Bacterial Fluorescence Labeling
  JOURNAL   PLoS ONE 11 (3), E0146827 (2016)
  PUBMED    26937640
REFERENCE   2  (bases 1 to 5039)
  AUTHORS   Barbier M, Damron FH.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2015) Microbiology, Immunology and Cell Biology, 
            West Virginia University, School of Medicine, One Medical Center 
            Drive, Morgantown, WV 26506, USA
REFERENCE   3  (bases 1 to 5039)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5039)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2016"; volume: "11"; issue: "3"; pages: "E0146827"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-OCT-2015) Microbiology, Immunology and Cell Biology, West 
            Virginia University, School of Medicine, One Medical Center Drive, 
            Morgantown, WV 26506, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5039
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..1058
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1232..1820
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     oriT            complement(1892..2000)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     protein_bind    2384..2405
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2420..2450
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2458..2474
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2482..2498
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     RBS             2538..2549
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2556..3269
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     terminator      3292..3386
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(3433..3449)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(3613..4443)
                     /label=pRO1600 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
     rep_origin      4457..4808
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacata agctagctta tcggccagcc tcgcagagca ggattcccgt
     1921 tgagcaccgc caggtgcgaa taagggacag tgaagaagga acacccgctc gcgggtgggc
     1981 ctacttcacc tatcctgccc ggctgacgcc gttggataca ccaaggaaag tctacacgaa
     2041 ccctttggca aaatcctgta tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta
     2101 taatgacccc gaagcagggt tatgcagcgg aaagtatacc ttaaggaatc cccatgttct
     2161 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     2221 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     2281 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     2341 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     2401 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     2461 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgaa ttcggcgcgc
     2521 caagaaggtc tagaattaaa gaggagaaat taagcatgcg taaaggagaa gaacttttca
     2581 ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg
     2641 tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca
     2701 ctactggaaa actacctgtt ccatggccaa cacttgtcac tactttcggt tatggtgttc
     2761 aatgctttgc gagataccca gatcatatga aacagcatga ctttttcaag agtgccatgc
     2821 ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac
     2881 gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg
     2941 attttaaaga agatggaaac attcttggac acaaattgga atacaactat aactcacaca
     3001 atgtatacat catggcagac aaacaaaaga atggaatcaa agttaacttc aaaattagac
     3061 acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat actccaattg
     3121 gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga
     3181 aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga
     3241 ttacacatgg catggatgaa ctatacaaat aagcttaatt agctgagctt ggactcctgt
     3301 tgatagatcc agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc
     3361 gccgggcgtt ttttattggt gagaatccaa gctggatcct ctagagtcga cctgcaggca
     3421 tgcaagcttg gcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     3481 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     3541 ccgcaccgat cgcccttccc aacagttgcg cagcctgaaa ggcaggccgg gccgtggtgg
     3601 ccacggcctc taggccagat ccagcggcat ctgggttagt cgagcgcggg ccgcttccca
     3661 tgtctcacca gggcgagcct gtttcgcgat ctcagcatct gaaatcttcc cggccttgcg
     3721 cttcgctggg gccttaccca ccgccttggc gggcttcttc ggtccaaaac tgaacaacag
     3781 atgtgtgacc ttgcgcccgg tctttcgctg cgcccactcc acctgtagcg ggctgtgctc
     3841 gttgatctgc gtcacggctg gatcaagcac tcgcaacttg aagtccttga tcgagggata
     3901 ccggccttcc agttgaaacc actttcgcag ctggtcaatt tctatttcgc gctggccgat
     3961 gctgtcccat tgcatgagca gctcgtaaag cctgatcgcg tgggtgctgt ccatcttggc
     4021 cacgtcagcc aaggcgtatt tggtgaactg tttggtgagt tccgtcaggt acggcagcat
     4081 gtctttggtg aacctgagtt ctacacggcc ctcaccctcc cggtagatga ttgtttgcac
     4141 ccagccggta atcatcacac tcggtctttt ccccttgcca ttgggctctt gggttaaccg
     4201 gacttcccgc cgtttcaggc gcagggccgc ttctttgagc tggttgtagg aagattcgat
     4261 agggacaccc gccatcgtcg ctatgtcctc cgccgtcact gaatacatca cttcatcggt
     4321 gacaggctcg ctcctcttca cctggctaat acaggccaga acgatccgct gttcctgaac
     4381 actgaggcga tacgcggcct cgaccagggc attgcttttg taaaccattg ggggtgaggc
     4441 cacgttcgac attccttgtg tataagggga cactgtatct gcgtcccaca atacaacaaa
     4501 tccgtccctt tacaacaaca aatccgtccc ttcttaacaa caaatccgtc ccttaatggc
     4561 aacaaatccg tcccttttta aactctacag gccacggatt acgtggcctg tagacgtcct
     4621 aaaaggttta aaagggaaaa ggaagaaaag ggtggaaacg caaaaaacgc accactacgt
     4681 ggccccgttg gggccgcatt tgtgcccctg aaggggcggg ggaggcgtct gggcaatccc
     4741 cgttttacca gtcccctatc gccgcctgag agggcgcagg aagcgagtaa tcagggtatc
     4801 gaggcggatt cacccttggc gtccaaccag cggcaccagc ggcgcctgag aggtatggtg
     4861 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     4921 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     4981 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcga
//
