LOCUS       Exported                4542 bp DNA     circular SYN 14-JUN-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4542)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4542)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4542
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(891..1242)
                     /direction=LEFT
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     CDS             1256..2086
                     /codon_start=1
                     /label=pRO1600 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
                     /translation="VASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     primer_bind     2236..2252
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(2256..2312)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2322..2337)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2345..2361)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2369..2399)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2414..2435)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2723..3311)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3485..4342)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4343..4447)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcggc
      241 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
      301 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
      361 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtggat ctcgcggtat
      421 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
      481 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
      541 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
      601 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
      661 cccttaacgt gagttttcgt tccactgagc gtcagacccc aattacacgc cactggctgt
      721 gcttgctggg gtgacggtgg caacggtggc ggccttgctg ggctatcgcg ttggaaagaa
      781 acgagggaaa ggggactgat aaaccggtct tagcccctcc ccttggtgtc caaccgctct
      841 gtaggcctct caggcgccgc tggtgccgct ggttggacgc caagggtgaa tccgcctcga
      901 taccctgatt actcgcttcc tgcgccctct caggcggcga taggggactg gtaaaacggg
      961 gattgcccag acgcctcccc cgccccttca ggggcacaaa tgcggcccca acggggccac
     1021 gtagtggtgc gttttttgcg tttccaccct tttcttcctt ttccctttta aaccttttag
     1081 gacgtctaca ggccacgtaa tccgtggcct gtagagttta aaaagggacg gatttgttgc
     1141 cattaaggga cggatttgtt gttaagaagg gacggatttg ttgttgtaaa gggacggatt
     1201 tgttgtattg tgggacgcag atacagtgtc cccttataca caaggaatgt cgaacgtggc
     1261 ctcaccccca atggtttaca aaagcaatgc cctggtcgag gccgcgtatc gcctcagtgt
     1321 tcaggaacag cggatcgttc tggcctgtat tagccaggtg aagaggagcg agcctgtcac
     1381 cgatgaagtg atgtattcag tgacggcgga ggacatagcg acgatggcgg gtgtccctat
     1441 cgaatcttcc tacaaccagc tcaaagaagc ggccctgcgc ctgaaacggc gggaagtccg
     1501 gttaacccaa gagcccaatg gcaaggggaa aagaccgagt gtgatgatta ccggctgggt
     1561 gcaaacaatc atctaccggg agggtgaggg ccgtgtagaa ctcaggttca ccaaagacat
     1621 gctgccgtac ctgacggaac tcaccaaaca gttcaccaaa tacgccttgg ctgacgtggc
     1681 caagatggac agcacccacg cgatcaggct ttacgagctg ctcatgcaat gggacagcat
     1741 cggccagcgc gaaatagaaa ttgaccagct gcgaaagtgg tttcaactgg aaggccggta
     1801 tccctcgatc aaggacttca agttgcgagt gcttgatcca gccgtgacgc agatcaacga
     1861 gcacagcccg ctacaggtgg agtgggcgca gcgaaagacc gggcgcaagg tcacacatct
     1921 gttgttcagt tttggaccga agaagcccgc caaggcggtg ggtaaggccc cagcgaagcg
     1981 caaggccggg aagatttcag atgctgagat cgcgaaacag gctcgccctg gtgagacatg
     2041 ggaagcggcc cgcgctcgac taacccagat gccgctggat ctggcctaga ggtgcaccac
     2101 ctgctttcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     2161 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
     2221 cccagtcacg acgttgtaaa acgacggcca gtgccaagct tgcatgcctg caggtcgact
     2281 ctagaggatc cccgggtacc gagctcgaat tcgtaatcat gtcatagctg tttcctgtgt
     2341 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     2401 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     2461 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     2521 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     2581 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     2641 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     2701 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     2761 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     2821 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     2881 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     2941 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     3001 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     3061 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     3121 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     3181 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     3241 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     3301 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     3361 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     3421 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     3481 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     3541 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     3601 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     3661 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     3721 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     3781 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     3841 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     3901 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     3961 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     4021 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     4081 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     4141 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     4201 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     4261 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     4321 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     4381 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     4441 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     4501 cattaaccta taaaaatagg cgtatcacga ggccctttcg tc
//
