LOCUS       40924_45288        9659 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pUCP18-RedS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9659)
  AUTHORS   Lesic B, Rahme LG.
  TITLE     Use of the lambda Red recombinase system to rapidly generate mutants
            in Pseudomonas aeruginosa
  JOURNAL   BMC Mol. Biol. 9, 20 (2008)
  PUBMED    18248677
REFERENCE   2  (bases 1 to 9659)
  AUTHORS   Lesic B, Hazan R, Rahme LG.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2007) Surgery, Mass General Hospital, 50 Blossom 
            Street, Boston, MA 02114, USA
REFERENCE   3  (bases 1 to 9659)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9659)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Mol. 
            Biol. 9, 20 (2008)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-JUL-2007) Surgery, Mass General Hospital, 50 Blossom Street, 
            Boston, MA 02114, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9659
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..1058
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1232..1820
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2144..2174
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2365..3240)
                     /codon_start=1
                     /label=araC
                     /note="L-arabinose regulatory protein"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        3267..3551
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     CDS             3610..4023
                     /codon_start=1
                     /label=Gam
                     /note="inhibitor of the host RecBCD nuclease in the lambda 
                     Red system"
                     /translation="MDINTETEIKQKHSLTPFPVFLISPAFRGRYFHSYFRSSAMNAYY
                     IQDRLEAQSWARHYQQLAREEKEAELADDMEKGLPQHLFESLCIDHLQRHGASKKSITR
                     AFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     CDS             4032..4814
                     /codon_start=1
                     /label=Beta
                     /note="single-stranded DNA binding recombinase in the 
                     lambda Red system"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQFI
                     ALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNESC
                     TCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLA
                     FGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLP
                     LCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
     CDS             4814..5491
                     /codon_start=1
                     /label=Exo
                     /note="5' to 3' double-stranded DNA exonuclease in the 
                     lambda
                     Red system"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKPR
                     SGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTESP
                     IIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQYSMW
                     VTRKNAWYFANYDPRMKREGLHYVVIERDEKYMASFDEIVPEFIEKMDEALAEIGFVFG
                     EQWR"
     primer_bind     complement(5521..5537)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(5701..6531)
                     /codon_start=1
                     /label=pRO1600 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
                     /translation="VASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     rep_origin      6545..6896
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     CDS             7980..9398
                     /codon_start=1
                     /label=SacB
                     /note="secreted levansucrase that renders bacterial growth 
                     sensitive to sucrose"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgaattcgag ctcggtaccc ggggatcctc tagagtcgac ctgcataatg
     2281 tgcctgtcaa atggacgaag cagggattct gcaaacccta tgctactccg tcaagccgtc
     2341 aattgtctga ttcgttacca attatgacaa cttgacggct acatcattca ctttttcttc
     2401 acaaccggca cggaactcgc tcgggctggc cccggtgcat tttttaaata cccgcgagaa
     2461 atagagttga tcgtcaaaac caacattgcg accgacggtg gcgataggca tccgggtggt
     2521 gctcaaaagc agcttcgcct ggctgatacg ttggtcctcg cgccagctta agacgctaat
     2581 ccctaactgc tggcggaaaa gatgtgacag acgcgacggc gacaagcaaa catgctgtgc
     2641 gacgctggcg atatcaaaat tgctgtctgc caggtgatcg ctgatgtact gacaagcctc
     2701 gcgtacccga ttatccatcg gtggatggag cgactcgtta atcgcttcca tgcgccgcag
     2761 taacaattgc tcaagcagat ttatcgccag cagctccgaa tagcgccctt ccccttgccc
     2821 ggcgttaatg atttgcccaa acaggtcgct gaaatgcggc tggtgcgctt catccgggcg
     2881 aaagaacccc gtattggcaa atattgacgg ccagttaagc cattcatgcc agtaggcgcg
     2941 cggacgaaag taaacccact ggtgatacca ttcgcgagcc tccggatgac gaccgtagtg
     3001 atgaatctct cctggcggga acagcaaaat atcacccggt cggcaaacaa attctcgtcc
     3061 ctgatttttc accaccccct gaccgcgaat ggtgagattg agaatataac ctttcattcc
     3121 cagcggtcgg tcgataaaaa aatcgagata accgttggcc tcaatcggcg ttaaacccgc
     3181 caccagatgg gcattaaacg agtatcccgg cagcagggga tcattttgcg cttcagccat
     3241 acttttcata ctcccgccat tcagagaaga aaccaattgt ccatattgca tcagacattg
     3301 ccgtcactgc gtcttttact ggctcttctc gctaaccaaa ccggtaaccc cgcttattaa
     3361 aagcattctg taacaaagcg ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta
     3421 taatcacggc agaaaagtcc acattgatta tttgcacggc gtcacacttt gctatgccat
     3481 agcattttta tccataagat tagcggatcc tacctgacgc tttttatcgc aactctctac
     3541 tgtttctcca tacccgtttt tttgggctag cgaaaagatg tttcgtgaag ccgtcgacgc
     3601 ttataaaaaa tggatattaa tactgaaact gagatcaagc aaaagcattc actaaccccc
     3661 tttcctgttt tcctaatcag cccggcattt cgcgggcgat attttcacag ctatttcagg
     3721 agttcagcca tgaacgctta ttacattcag gatcgtcttg aggctcagag ctgggcgcgt
     3781 cactaccagc agctcgcccg tgaagagaaa gaggcagaac tggcagacga catggaaaaa
     3841 ggcctgcccc agcacctgtt tgaatcgcta tgcatcgatc atttgcaacg ccacggggcc
     3901 agcaaaaaat ccattacccg tgcgtttgat gacgatgttg agtttcagga gcgcatggca
     3961 gaacacatcc ggtacatggt tgaaaccatt gctcaccacc aggttgatat tgattcagag
     4021 gtataaaacg aatgagtact gcactcgcaa cgctggctgg gaagctggct gaacgtgtcg
     4081 gcatggattc tgtcgaccca caggaactga tcaccactct tcgccagacg gcatttaaag
     4141 gtgatgccag cgatgcgcag ttcatcgcat tactgatcgt tgccaaccag tacggcctta
     4201 atccgtggac gaaagaaatt tacgcctttc ctgataagca gaatggcatc gttccggtgg
     4261 tgggcgttga tggctggtcc cgcatcatca atgaaaacca gcagtttgat ggcatggact
     4321 ttgagcagga caatgaatcc tgtacatgcc ggatttaccg caaggaccgt aatcatccga
     4381 tctgcgttac cgaatggatg gatgaatgcc gccgcgaacc attcaaaact cgcgaaggca
     4441 gagaaatcac ggggccgtgg cagtcgcatc ccaaacggat gttacgtcat aaagccatga
     4501 ttcagtgtgc ccgtctggcc ttcggatttg ctggtatcta tgacaaggat gaagccgagc
     4561 gcattgtcga aaatactgca tacactgcag aacgtcagcc ggaacgcgac atcactccgg
     4621 ttaacgatga aaccatgcag gagattaaca ctctgctgat cgccctggat aaaacatggg
     4681 atgacgactt attgccgctc tgttcccaga tatttcgccg cgacattcgt gcatcgtcag
     4741 aactgacaca ggccgaagca gtaaaagctc ttggattcct gaaacagaaa gccgcagagc
     4801 agaaggtggc agcatgacac cggacattat cctgcagcgt accgggatcg atgtgagagc
     4861 tgtcgaacag ggggatgatg cgtggcacaa attacggctc ggcgtcatca ccgcttcaga
     4921 agttcacaac gtgatagcaa aaccccgctc cggaaagaag tggcctgaca tgaaaatgtc
     4981 ctacttccac accctgcttg ctgaggtttg caccggtgtg gctccggaag ttaacgctaa
     5041 agcactggcc tggggaaaac agtacgagaa cgacgccaga accctgtttg aattcacttc
     5101 cggcgtgaat gttactgaat ccccgatcat ctatcgcgac gaaagtatgc gtaccgcctg
     5161 ctctcccgat ggtttatgca gtgacggcaa cggccttgaa ctgaaatgcc cgtttacctc
     5221 ccgggatttc atgaagttcc ggctcggtgg tttcgaggcc ataaagtcag cttacatggc
     5281 ccaggtgcag tacagcatgt gggtgacgcg aaaaaatgcc tggtactttg ccaactatga
     5341 cccgcgtatg aagcgtgaag gcctgcatta tgtcgtgatt gagcgggatg aaaagtacat
     5401 ggcgagtttt gacgagatcg tgccggagtt catcgaaaaa atggacgagg cactggctga
     5461 aattggtttt gtatttgggg agcaatggcg atgacgcatc ctcacgataa taagcttggc
     5521 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     5581 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     5641 cccttcccaa cagttgcgca gcctgaaagg caggccgggc cgtggtggcc acggcctcta
     5701 ggccagatcc agcggcatct gggttagtcg agcgcgggcc gcttcccatg tctcaccagg
     5761 gcgagcctgt ttcgcgatct cagcatctga aatcttcccg gccttgcgct tcgctggggc
     5821 cttacccacc gccttggcgg gcttcttcgg tccaaaactg aacaacagat gtgtgacctt
     5881 gcgcccggtc tttcgctgcg cccactccac ctgtagcggg ctgtgctcgt tgatctgcgt
     5941 cacggctgga tcaagcactc gcaacttgaa gtccttgatc gagggatacc ggccttccag
     6001 ttgaaaccac tttcgcagct ggtcaatttc tatttcgcgc tggccgatgc tgtcccattg
     6061 catgagcagc tcgtaaagcc tgatcgcgtg ggtgctgtcc atcttggcca cgtcagccaa
     6121 ggcgtatttg gtgaactgtt tggtgagttc cgtcaggtac ggcagcatgt ctttggtgaa
     6181 cctgagttct acacggccct caccctcccg gtagatgatt gtttgcaccc agccggtaat
     6241 catcacactc ggtcttttcc ccttgccatt gggctcttgg gttaaccgga cttcccgccg
     6301 tttcaggcgc agggccgctt ctttgagctg gttgtaggaa gattcgatag ggacacccgc
     6361 catcgtcgct atgtcctccg ccgtcactga atacatcact tcatcggtga caggctcgct
     6421 cctcttcacc tggctaatac aggccagaac gatccgctgt tcctgaacac tgaggcgata
     6481 cgcggcctcg accagggcat tgcttttgta aaccattggg ggtgaggcca cgttcgacat
     6541 tccttgtgta taaggggaca ctgtatctgc gtcccacaat acaacaaatc cgtcccttta
     6601 caacaacaaa tccgtccctt cttaacaaca aatccgtccc ttaatggcaa caaatccgtc
     6661 cctttttaaa ctctacaggc cacggattac gtggcctgta gacgtcctaa aaggtttaaa
     6721 agggaaaagg aagaaaaggg tggaaacgca aaaaacgcac cactacgtgg ccccgttggg
     6781 gccgcatttg tgcccctgaa ggggcggggg aggcgtctgg gcaatccccg ttttaccagt
     6841 cccctatcgc cgcctgagag ggcgcaggaa gcgagtaatc agggtatcga ggcggattca
     6901 cccttggcgt ccaaccagcg gcaccagcgg cgcctgagag gcctacagag cggttggaca
     6961 ccaaggggag gggctaagac cggtttatca gtcccctttc cctcgtttct ttccaacgcg
     7021 atagcccagc aaggccgcca ccgttgccac cgtcacccca gcaagcacag ccagtggcgt
     7081 gtaattgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     7141 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     7201 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     7261 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     7321 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagatcc
     7381 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     7441 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     7501 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgccg cctgatgcgg
     7561 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggcaact ttatgcccat
     7621 gcaacagaaa ctataaaaaa tacagagaat gaaaagaaac agatagattt tttagttctt
     7681 taggcccgta gtctgcaaat ccttttatga ttttctatca aacaaaagag gaaaatagac
     7741 cagttgcaat ccaaacgaga gtctaataga atgaggtcga aaagtaaatc gcgcgggttt
     7801 gttactgata aagcaggcaa gacctaaaat gtgtaaaggg caaagtgtat actttggcgt
     7861 caccccttac atattttagg tcttttttta ttgtgcgtaa ctaacttgcc atcttcaaac
     7921 acaggagggc tggaagaagc agaccgctaa cacagtacat aaaaaaggag acatgaacga
     7981 tgaacatcaa aaagtttgca aaacaagcaa cagtattaac ctttactacc gcactgctgg
     8041 caggaggcgc aactcaagcg tttgcgaaag aaacgaacca aaagccatat aaggaaacat
     8101 acggcatttc ccatattaca cgccatgata tgctgcaaat ccctgaacag caaaaaaatg
     8161 aaaaatatca agttcctgaa ttcgattcgt ccacaattaa aaatatctct tctgcaaaag
     8221 gcctggacgt ttgggacagc tggccattac aaaacgctga cggcactgtc gcaaactatc
     8281 acggctacca catcgtcttt gcattagccg gagatcctaa aaatgcggat gacacatcga
     8341 tttacatgtt ctatcaaaaa gtcggcgaaa cttctattga cagctggaaa aacgctggcc
     8401 gcgtctttaa agacagcgac aaattcgatg caaatgattc tatcctaaaa gaccaaacac
     8461 aagaatggtc aggttcagcc acatttacat ctgacggaaa aatccgttta ttctacactg
     8521 atttctccgg taaacattac ggcaaacaaa cactgacaac tgcacaagtt aacgtatcag
     8581 catcagacag ctctttgaac atcaacggtg tagaggatta taaatcaatc tttgacggtg
     8641 acggaaaaac gtatcaaaat gtacagcagt tcatcgatga aggcaactac agctcaggcg
     8701 acaaccatac gctgagagat cctcactacg tagaagataa aggccacaaa tacttagtat
     8761 ttgaagcaaa cactggaact gaagatggct accaaggcga agaatcttta tttaacaaag
     8821 catactatgg caaaagcaca tcattcttcc gtcaagaaag tcaaaaactt ctgcaaagcg
     8881 ataaaaaacg cacggctgag ttagcaaacg gcgctctcgg tatgattgag ctaaacgatg
     8941 attacacact gaaaaaagtg atgaaaccgc tgattgcatc taacacagta acagatgaaa
     9001 ttgaacgcgc gaacgtcttt aaaatgaacg gcaaatggta cctgttcact gactcccgcg
     9061 gatcaaaaat gacgattgac ggcattacgt ctaacgatat ttacatgctt ggttatgttt
     9121 ctaattcttt aactggccca tacaagccgc tgaacaaaac tggccttgtg ttaaaaatgg
     9181 atcttgatcc taacgatgta acctttactt actcacactt cgctgtacct caagcgaaag
     9241 gaaacaatgt cgtgattaca agctatatga caaacagagg attctacgca gacaaacaat
     9301 caacgtttgc gccaagcttc ctgctgaaca tcaaaggcaa gaaaacatct gttgtcaaag
     9361 acagcatcct tgaacaagga caattaacag ttaacaaata aaaacgcaaa agaaaatgcc
     9421 gatatcctat tggcattttc ttttatttct tatcaacata aaggtgaatc ccatatggtg
     9481 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     9541 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     9601 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcga
//
