LOCUS       62056_22340        2982 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..2982
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(86..474)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     protein_bind    complement(495..528)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    complement(718..766)
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        complement(902..1011)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        1056..1147
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    1406..1540
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     terminator      1626..1673
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        1911..2013
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             2014..2670
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 tctgcggact ggctttctac gtgttccgct tcctttagca gcccttgcgc cctgagtgct
       61 tgcggcagcg tgaagctaat tcccatgtca gccgttaagt gttcctgtgt cactcaaaat
      121 tgctttgaga ggctctaagg gcttctcagt gcgttacatc cctggcttgt tgtccacaac
      181 cgttaaacct taaaagcttt aaaagcctta tatattcttt tttttcttat aaaacttaaa
      241 accttagagg ctatttaagt tgctgattta tattaatttt attgttcaaa catgagagct
      301 tagtacgtga aacatgagag cttagtacgt tagccatgag agcttagtac gttagccatg
      361 agggtttagt tcgttaaaca tgagagctta gtacgttaaa catgagagct tagtacgtga
      421 aacatgagag cttagtacgt actatcaaca ggttgaactg ctgatcaaca gatcctctac
      481 gcggccgcgg taccataact tcgtatagca tacattatac gaagttatct ggtttaaacg
      541 tacccgtagt ggctatggca gggcttgccg ccccgacgtt ggctgcgagc cctgggcctt
      601 cacccgaact tgggggttgg ggtggggaaa aggaagaaac gcgggcgtat tggtcccaat
      661 ggggtctcgg tggggtatcg acagagtgcc agccctggga ccgaaccccg cgtttatgaa
      721 caaacgaccc aacacccgtg cgttttattc tgtcttttta ttgccgtcat agcgcgggtt
      781 ccttccggta ttgtctcctt ccgtgtttca gttagcctcc cccatctccc ggtaccgcat
      841 gctatgcatc agctgctagc accatggctc gagatcccgg gtgatcaagt cttcgtcgag
      901 tgattgtaaa taaaatgtaa tttacagtat agtattttaa ttaatataca aatgatttga
      961 taataattct tatttaacta taatatattg tgttgggttg aattaaaggt ccgtatacta
     1021 gtatcgattc gcgacctact ccggaatatt aatagatcat ggagataatt aaaatgataa
     1081 ccatctcgca aataaataag tattttactg ttttcgtaac agttttgtaa taaaaaaacc
     1141 tataaatatt ccggattatt cataccgtcc caccatcggg cgcggatccc ggtccgaagc
     1201 gcgcggaatt caaaggccta cgtcgacgag ctcactagtc gcggccgctt tcgaatctag
     1261 agcctgcagt ctcgacaagc ttgtcgagaa gtactagagg atcataatca gccataccac
     1321 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     1381 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     1441 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     1501 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc tgatcactgc ttgagcctag
     1561 aagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
     1621 aataactatc ataaccccta ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
     1681 gaggaactat atccggatct gaacaggagg gacagctgat agaaacagaa gccactggag
     1741 cacctcaaaa acaccatcat acactaaatc agtaagttgg cagcatcacc cgacgcactt
     1801 tgcgccgaat aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc
     1861 cctgttgata ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac
     1921 gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag
     1981 ttatcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
     2041 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
     2101 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag
     2161 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
     2221 catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
     2281 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
     2341 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa
     2401 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc
     2461 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc
     2521 gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt
     2581 caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa
     2641 cagtactgcg atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct
     2701 taaacgcctg gttgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg
     2761 aaagcaaatt cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct
     2821 attgctggtt taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg
     2881 cctgaggcca gtttgctcag gctctccccg tggaggtaat aattgacgat atgatcattt
     2941 attctgcctc ccagctgaca ttcatccggg gtcagcaccg tt
//
