LOCUS       40924_45233        6175 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pUCAG.nkx2.5, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6175)
  AUTHORS   van Tuyn J, Pijnappels DA, de Vries AA, de Vries I, van der 
            Velde-van Dijke I, Knaan-Shanzer S, van der Laarse A, Schalij MJ, 
            Atsma DE.
  TITLE     Fibroblasts from human postmyocardial infarction scars acquire 
            properties of cardiomyocytes after transduction with a recombinant 
            myocardin gene
  JOURNAL   FASEB J. 21 (12), 3369-3379 (2007)
  PUBMED    17579192
REFERENCE   2  (bases 1 to 6175)
  AUTHORS   van Tuyn J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2006) MCB, LUMC, Einthovenweg 20, Leiden, ZH 2300 
            RC, Netherlands
REFERENCE   3  (bases 1 to 6175)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6175)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FASEB J."; 
            date: "2007"; volume: "21"; issue: "12"; pages: "3369-3379"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-DEC-2006) MCB, LUMC, Einthovenweg 20, Leiden, ZH 2300 RC, 
            Netherlands"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6175
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      68..448
                     /label=CMV.IE enhancer
                     /note="CMV.IE enhancer"
                     /regulatory_class="enhancer"
     enhancer        68..447
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        450..725
                     /label=chicken beta-actin promoter
     intron          726..1734
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     CDS             1845..2816
                     /gene="NKX2-5"
                     /label=NKX2-5
                     /note="Homeobox protein Nkx-2.5 from Homo sapiens.
                     Accession#: P52952"
     polyA_signal    3144..3199
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3576..3592)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4066..4170
                     /label=AmpR promoter
     CDS             4171..5028
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5202..5790
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    6078..6099
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6114..6144
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6152..6168
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 caggaaacag ctatgaccat gattacgcca agcttgttta aacatttaaa tctcgagcca
       61 tggattcgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
      121 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      181 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      241 atagggactt tccattgacg tcaatgggtg gactatttac ggtaaactgc ccacttggca
      301 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      361 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      421 tacgtattag tcatcgctat taccatgggt cgaggtgagc cccacgttct gcttcactct
      481 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg
      541 tgcagcgatg ggggcggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg
      601 ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa
      661 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc
      721 gggcgggagt cgctgcgttg ccttcgcccc gtgccccgct ccgcgccgcc tcgcgccgcc
      781 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
      841 ctccgggctg taattagcgc ttggtttaat gacggctcgt ttcttttctg tggctgcgtg
      901 aaagccttaa agggctccgg gagggccctt tgtgcggggg ggagcggctc ggggggtgcg
      961 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggcccgc gctgcccggc ggctgtgagc
     1021 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcgtgtg cgcgagggga gcgcggccgg
     1081 gggcggtgcc ccgcggtgcg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
     1141 tgcgtggggg ggtgagcagg gggtgtgggc gcggcggtcg ggctgtaacc cccccctgca
     1201 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtgcggggcg
     1261 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1321 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccg gagcgccggc
     1381 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg
     1441 cagggacttc ctttgtccca aatctggcgg agccgaaatc tgggaggcgc cgccgcaccc
     1501 cctctagcgg gcgcgggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg
     1561 ccttcgtgcg tcgccgcgcc gccgtcccct tctccatctc cagcctcggg gctgccgcag
     1621 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     1681 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     1741 ggcaacgtgc tggttgttgt gctgtctcat cattttggca aagaattatc gcatgcctgc
     1801 agagctctag agtcacctgg cgctgtgaga ctggcgctgc caccatgttc cccagccctg
     1861 ctctcacgcc cacgcccttc tcagtcaaag acatcctaaa cctggagcag cagcagcgca
     1921 gcctggctgc cgccggagag ctctctgccc gcctggaggc gaccctggcg ccctcctcct
     1981 gcatgctggc cgccttcaag ccagaggcct acgctgggcc cgaggcggct gcgccgggcc
     2041 tcccagagct gcgcgcagag ctgggccgcg cgccttcacc ggccaagtgt gcgtctgcct
     2101 ttcccgccgc ccccgccttc tatccacgtg cctacagcga ccccgaccca gccaaggacc
     2161 ctagagccga aaagaaagag ctgtgcgcgc tgcagaaggc ggtggagctg gagaagacag
     2221 aggcggacaa cgcggagcgg ccccgggcgc gacggcggag gaagccgcgc gtgctcttct
     2281 cgcaggcgca ggtctatgaa ctggagcggc gcttcaagca acagcggtac ctgtcggccc
     2341 ccgaacgcga ccagctggcc agcgtgctga aactcacgtc cacgcaggtc aagatctggt
     2401 tccagaaccg gcgctacaag tgcaagcggc agcggcagga ccagactctg gagctggtgg
     2461 ggctgccccc gccgccgccg ccgcctgccc gcaggatcgc ggtgccagtg ctggtgcgcg
     2521 atggcaagcc atgcctaggg gactcggcgc cctacgcgcc tgcctacggc gtgggcctca
     2581 atccctacgg ttataacgcc taccccgcct atccgggtta cggcggcgcg gcctgcagcc
     2641 ctggctacag ctgcactgcc gcttaccccg ccgggccttc cccagcgcag ccggccactg
     2701 ccgccgccaa caacaacttc gtgaacttcg gcgtcgggga cttgaatgcg gttcagagcc
     2761 ccgggattcc gcagagcaac tcgggagtgt ccacgctgca tggtatccga gcctggtagg
     2821 gaagggaccc gcgtggcgcg accctgaccg atcccacctc aacagctccc tgactctcgg
     2881 ggggagaagg ggctcccaac atgaccctga gtcccctgga ttttgcattc actcctgcgg
     2941 agacctagga actgacgggc ccggtaccgc ggccgcactt aagttacgcg tggatcaatt
     3001 cactcctcag gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca atgccctggc
     3061 tcacaaatac cactgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc
     3121 ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg
     3181 gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca
     3241 gaatgagtat ttggtttaga gtttggcaac atatgccata tgctggctgc catgaacaaa
     3301 ggtggctata aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc
     3361 atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt
     3421 ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct
     3481 gactactccc agtcatagct gtccctcttc tcttatgaag atccctcgac ctgcagccca
     3541 agctgatccc gggatttaaa tgtttaaacg aattcactgg ccgtcgtttt acaacgtcgt
     3601 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     3661 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     3721 aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     3781 cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     3841 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     3901 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     3961 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     4021 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     4081 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     4141 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     4201 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     4261 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     4321 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     4381 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     4441 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     4501 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     4561 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     4621 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     4681 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     4741 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     4801 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     4861 gataaatctg gagcccgtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     4921 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     4981 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     5041 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     5101 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     5161 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     5221 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     5281 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     5341 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     5401 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     5461 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     5521 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     5581 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     5641 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     5701 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     5761 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     5821 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     5881 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     5941 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     6001 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     6061 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     6121 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcaca
//
