LOCUS       40924_45228        8054 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pUCAG.myocardin, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8054)
  AUTHORS   van Tuyn J, Pijnappels DA, de Vries AA, de Vries I, van der 
            Velde-van Dijke I, Knaan-Shanzer S, van der Laarse A, Schalij MJ, 
            Atsma DE.
  TITLE     Fibroblasts from human postmyocardial infarction scars acquire 
            properties of cardiomyocytes after transduction with a recombinant 
            myocardin gene
  JOURNAL   FASEB J. 21 (12), 3369-3379 (2007)
  PUBMED    17579192
REFERENCE   2  (bases 1 to 8054)
  AUTHORS   van Tuyn J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2006) MCB, LUMC, Einthovenweg 20, Leiden, ZH 2300 
            RC, Netherlands
REFERENCE   3  (bases 1 to 8054)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8054)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FASEB J."; 
            date: "2007"; volume: "21"; issue: "12"; pages: "3369-3379"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-DEC-2006) MCB, LUMC, Einthovenweg 20, Leiden, ZH 2300 RC, 
            Netherlands"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8054
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      68..448
                     /label=CMV.IE enhancer
                     /note="CMV.IE enhancer"
                     /regulatory_class="enhancer"
     enhancer        68..447
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        450..725
                     /label=chicken beta-actin promoter
     intron          726..1734
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     CDS             1852..4812
                     /codon_start=1
                     /gene="MYOCD"
                     /product="Homo sapiens myocardin"
                     /label=MYOCD
                     /note="similar to myocardin deposited in GenBank Accession 
                     AY764180"
                     /protein_id="ABM67537.1"
                     /translation="MTLLGSEHSLLIRSKFRSVLQLRLQQRRTQEQLANQGIIPPLKRP
                     AEFHEQRKHLDSDKAKNSLKRKARNRCNSADLVNMHILQASTAERSIPTAQMKLKRARL
                     ADDLNEKIALRPGPLELVEKNILPVDSAVKEAIKGNQVSFSKSTDAFAFEEDSSSDGLS
                     PDQTRSEDPQNSAGSPPDAKASDTPSTGSLGTNQDLASGSENDRNDSASQPSHQSDAGK
                     QGLGPPSTPIAVHAAVKSKSLGDSKNRHKKPKDPKPKVKKLKYHQYIPPDQKAEKSPPP
                     MDSAYARLLQQQQLFLQLQILSQQQQQQQHRFSYLGMHQAQLKEPNEQMVRNPNSSSTP
                     LSNTPLSPVKNSFSGQTGVSSFKPGPLPPNLDDLKVSELRQQLRIRGLPVSGTKTALMD
                     RLRPFQDCSGNPVPNFGDITTVTFPVTPNTLPNYQSSSSTSALSNGFYHFGSTSSSPPI
                     SPASSDLSVAGSLPDTFNDASPSFGLHPSPVHVCTEESLMSSLNGGSVPSELDGLDSEK
                     DKMLVEKQKVINELTWKLQQEQRQVEELRMQLQKQKRNNCSEKKPLPFLAASIKQEEAV
                     SSCPFASQVPVKRQSSSSECHPPACEAAQLQPLGNAHCVESSDQTNVLSSTFLSPQCSP
                     QHSPLGAVKSPQHISLPPSPNNPHFLPSSSGAQGEGHRVSSPISSQVCTAQNSGAHDGH
                     PPSFSPHSSSLHPPFSGAQADSSHGAGGNPCPKSPCVQQKMAGLHSSDKVGPKFSIPSP
                     TFSKSSSAISEVTQPPSYEDAVKQQMTRSQQMDELLDVLIESGEMPADAREDHSCLQKV
                     PKIPRSSRSPTAVLTKPSASFEQASSGSQIPFDPYATDSDEHLEVLLNSQSPLGKMSDV
                     TLLKIGSEEPHFDGIMDGFSGKAAEDLFNAHEILPGPLSPMQTQFSPSSVDSNGLQLSF
                     TESPWETMEWLDLTPPNSTPGFSALTTSSPSIFNIDFLDVTDLNLNSSMDLHLQQW"
     gene            1852..4812
                     /gene="MYOCD"
                     /label=MYOCD
     polyA_signal    5023..5078
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5455..5471)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5945..6049
                     /label=AmpR promoter
     CDS             6050..6907
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      7081..7669
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    7957..7978
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7993..8023
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8031..8047
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 caggaaacag ctatgaccat gattacgcca agcttgttta aacatttaaa tctcgagcca
       61 tggattcgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
      121 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      181 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      241 atagggactt tccattgacg tcaatgggtg gactatttac ggtaaactgc ccacttggca
      301 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      361 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      421 tacgtattag tcatcgctat taccatgggt cgaggtgagc cccacgttct gcttcactct
      481 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg
      541 tgcagcgatg ggggcggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg
      601 ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa
      661 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc
      721 gggcgggagt cgctgcgttg ccttcgcccc gtgccccgct ccgcgccgcc tcgcgccgcc
      781 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
      841 ctccgggctg taattagcgc ttggtttaat gacggctcgt ttcttttctg tggctgcgtg
      901 aaagccttaa agggctccgg gagggccctt tgtgcggggg ggagcggctc ggggggtgcg
      961 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggcccgc gctgcccggc ggctgtgagc
     1021 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcgtgtg cgcgagggga gcgcggccgg
     1081 gggcggtgcc ccgcggtgcg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
     1141 tgcgtggggg ggtgagcagg gggtgtgggc gcggcggtcg ggctgtaacc cccccctgca
     1201 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtgcggggcg
     1261 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1321 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccg gagcgccggc
     1381 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg
     1441 cagggacttc ctttgtccca aatctggcgg agccgaaatc tgggaggcgc cgccgcaccc
     1501 cctctagcgg gcgcgggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg
     1561 ccttcgtgcg tcgccgcgcc gccgtcccct tctccatctc cagcctcggg gctgccgcag
     1621 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     1681 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     1741 ggcaacgtgc tggttgttgt gctgtctcat cattttggca aagaattatc gcatgcctgc
     1801 agagctctag agtcgcctgg ccaagggacc agcggcttgc tgagactcaa catgacactc
     1861 ctggggtctg agcattcctt gctgattagg agcaagttca gatcagtttt acagttaaga
     1921 cttcaacaaa gaaggaccca ggaacaactg gctaaccaag gcataatacc accactgaaa
     1981 cgtccagctg aattccatga gcaaagaaaa catttggata gtgacaaggc taaaaattcc
     2041 ctgaagcgca aagccagaaa caggtgcaac agtgccgact tggttaatat gcacatactc
     2101 caagcttcca ctgccgagag gtccattcca actgctcaga tgaagctgaa aagagcccga
     2161 ctcgccgatg atctcaatga aaaaattgct ctacgaccag ggccactgga gctggtggaa
     2221 aaaaacattc ttcctgtgga ttctgctgtg aaagaggcca taaaaggtaa ccaggtgagt
     2281 ttctccaaat ccacggatgc ttttgccttt gaagaggaca gcagcagcga tgggctttct
     2341 ccggatcaga ctcgaagtga agacccccaa aactcagcgg gatccccgcc agacgctaaa
     2401 gcctcagata ccccttcgac aggttctctg gggacaaacc aggatcttgc ttctggctca
     2461 gaaaatgaca gaaatgactc agcctcacag cccagccacc agtcagatgc ggggaagcag
     2521 gggcttggcc cccccagcac ccccatagcc gtgcatgctg ctgtaaagtc caaatccttg
     2581 ggtgacagta agaaccgcca caaaaagccc aaggacccca agccaaaggt gaagaagctt
     2641 aaatatcacc agtacattcc cccagaccag aaggcagaga agtcccctcc acctatggac
     2701 tcagcctacg ctcggctgct ccagcaacag cagctgttcc tgcagctcca aatcctcagc
     2761 cagcagcagc agcagcagca acaccgattc agctacctag ggatgcacca agctcagctt
     2821 aaggaaccaa atgaacagat ggtcagaaat ccaaactctt cttcaacgcc actgagcaat
     2881 acccccttgt ctcctgtcaa aaacagtttt tctggacaaa ctggtgtctc ttctttcaaa
     2941 ccaggcccac tcccacctaa cctggatgat ctgaaggtct ctgaattaag acaacagctt
     3001 cgaattcggg gcttgcctgt gtcaggcacc aaaacggctc tcatggaccg gcttcgaccc
     3061 ttccaggact gctctggcaa cccagtgccg aactttgggg atataacgac tgtcactttt
     3121 cctgtcacac ccaacacgct gcccaattac cagtcttcct cttctaccag tgccctgtcc
     3181 aacggcttct accactttgg cagcaccagc tccagccccc cgatctcccc agcctcctct
     3241 gacctgtcag tcgctgggtc cctgccggac accttcaatg atgcctcccc ctccttcggc
     3301 ctgcacccgt ccccagtcca cgtgtgcacg gaggaaagtc tcatgagcag cctgaatggg
     3361 ggctctgttc cttctgagct ggatgggctg gactccgaga aggacaagat gctggtggag
     3421 aagcagaagg tgatcaatga actcacctgg aaactccagc aagagcagag gcaggtggag
     3481 gagctgagga tgcagcttca gaagcagaaa aggaataact gttcagagaa gaagccgctg
     3541 cctttcctgg ctgcctccat caagcaggaa gaggctgtct ccagctgtcc ttttgcatcc
     3601 caagtacctg tgaaaagaca aagcagcagc tcagagtgtc acccaccggc ttgtgaagct
     3661 gctcaactcc agcctcttgg aaatgctcat tgtgtggagt cctcagatca aaccaatgta
     3721 ctttcttcca catttctcag cccccagtgt tcccctcagc attcaccgct gggggctgtg
     3781 aaaagcccac agcacatcag tttgccccca tcacccaaca accctcactt tctgccctca
     3841 tcctccgggg cccagggaga agggcacagg gtctcctcgc ccatcagcag ccaggtgtgc
     3901 actgcacaga actcaggagc acacgatggc catcctccaa gcttctctcc ccattcttcc
     3961 agcctccacc cgcccttctc tggagcccaa gcagacagca gtcatggtgc cgggggaaac
     4021 ccttgtccca aaagcccatg tgtacagcaa aagatggctg gtttacactc ttctgataag
     4081 gtggggccaa agttttcaat tccatcccca actttttcta agtcaagttc agcaatttca
     4141 gaggtaacac agcctccatc ctatgaagat gccgtaaagc agcaaatgac ccggagtcag
     4201 cagatggatg aactcctgga cgtgcttatt gaaagcggag aaatgccagc agacgctaga
     4261 gaggatcact catgtcttca aaaagtccca aagataccca gatcttcccg aagtccaact
     4321 gctgtcctca ccaagccctc ggcttccttt gaacaagcct cttcaggcag ccagatcccc
     4381 tttgatccct atgccaccga cagtgatgag catcttgaag tcttattaaa ttcccagagc
     4441 cccctaggaa agatgagtga tgtcaccctt ctaaaaattg ggagcgaaga gcctcacttt
     4501 gatgggataa tggatggatt ctctgggaag gctgcagaag acctcttcaa tgcacatgag
     4561 atcttgccag gccccctctc tccaatgcag acacagtttt caccctcttc tgtggacagc
     4621 aatgggctgc agttaagctt cactgaatct ccctgggaaa ccatggagtg gctggacctc
     4681 actccgccaa attccacacc aggctttagc gccctcacca ccagcagccc cagcatcttc
     4741 aacatcgatt tcctggatgt cactgatctc aatttgaatt cttccatgga ccttcacttg
     4801 cagcagtggt agaatgccca atgcaccagt gctatggaag accaatggag aaatcccggg
     4861 atccacgcgt ggatcaattc actcctcagg tgcaggctgc ctatcagaag gtggtggctg
     4921 gtgtggccaa tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt
     4981 atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt
     5041 tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg
     5101 caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgccatat
     5161 gctggctgcc atgaacaaag gtggctataa agaggtcatc agtatatgaa acagccccct
     5221 gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt
     5281 tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc
     5341 agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct cttatgaaga
     5401 tccctcgacc tgcagcccaa gctgatcccg ggatttaaat gtttaaacga attcactggc
     5461 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     5521 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     5581 ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca
     5641 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
     5701 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     5761 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     5821 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     5881 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     5941 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     6001 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     6061 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     6121 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     6181 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     6241 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     6301 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     6361 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     6421 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     6481 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     6541 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     6601 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     6661 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     6721 ggctggctgg tttattgctg ataaatctgg agcccgtgag cgtgggtctc gcggtatcat
     6781 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     6841 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     6901 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     6961 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     7021 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     7081 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     7141 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     7201 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     7261 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     7321 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     7381 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     7441 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     7501 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     7561 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     7621 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     7681 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     7741 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     7801 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     7861 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     7921 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     7981 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     8041 ataacaattt caca
//
