LOCUS       V014794                11699 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014794
VERSION     V014794
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11699)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11699
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label="AmpR promoter"
     CDS             201..1058
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1232..1820
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    2108..2129
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2144..2174
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     CDS             6998..8311
                     /gene="pepE"
                     /label="Aminopeptidase E"
                     /note="Aminopeptidase E from Lactobacillus helveticus.
                     Accession#: P94870"
     CDS             complement(8729..8740)
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     CDS             10510..11157
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccaagctt agatctagtc ttataactat actgacaata gaaacattaa
     2281 caaatctaaa acagtcttaa ttctatcttg agaaagtatt ggtaataata ttattgtcga
     2341 taacgcgagc ataataaacg gctctgatta aattctgaag tttgttagat acaatgattt
     2401 cgttcgaagg aactacaaaa taaattataa ggaggcactc accatggatg aaatttaata
     2461 aaaaaagagt tgcaatagcc acgtttattg ctttgatatt tgtaagtttt tttacaatat
     2521 catcaatcca agataaccaa actaatgcag ccgaactttc ttccactcgt tcaattgata
     2581 tgagaagata tttagctgta cgtggcggtg caggggatgt tgctgaacct gatttaaacg
     2641 ctaagccaca agataattta tatttagccg tcaattccga atggttgtct aaagcagaaa
     2701 ttcctgcaga tcaaacttct gccggagtaa atactgaatt agatattaaa attgaaaaac
     2761 gcatgatgaa agattttgcg gatattgcgt ctggtaagga aaagatgcca gatattcgtg
     2821 acttcgataa ggcgattgcc ttgtataaga ttgcgaagaa ttttgataaa agggatgctg
     2881 aaaaggcaaa cccaattcaa aatgatttac aaaagatcct tgatttaatt aactttgata
     2941 aatttaagga taatgcaaca gagctcttca tgggtccata tgccttgcct tttgtatttg
     3001 atatagatgc tgacatgaaa aatacagatt ttaatgtctt gcattttggt ggtccaagca
     3061 catttttacc agatacaact acttataaga cacctgaagc caaaaaatta cttgatattt
     3121 tagaaaaaca aagtatcaac ttgttagaga tggcaggtat tggtaaagaa gaagcacgtg
     3181 tttatgttca agatgcttta gcctttgatc aaaaattatc caaggtcgtc aagtctactg
     3241 aagaatggtc agattatgct gcaatctata atccggtttc tttgactgaa ttcttagcta
     3301 agtttaagtc atttgatatg gctgactttt taaagacaat tttgcctgaa aaagttgaac
     3361 gagtaattgt aatggaacca cgtttccttg accatgctga tgaattaatt aatccggcaa
     3421 actttgatga aattaaaggc tggatgctgg ttaaatatat taatagcgta gctaagtact
     3481 tgtcacaaga tttccgtgcc gctgcatttc catttaacca agcaatttca ggtacgccag
     3541 aattgccttc tcaaattaag caagcctatc gtttagctaa tggtgctttt gatgaagttg
     3601 ttggtatttt ttatggtaag aaatactttg gtgaagaagc taaacacgat gtcgaagata
     3661 tgattcacaa tatgcttaaa gtatatgagc aaagaatcaa tgataataat tggttatctg
     3721 aagatactaa gaaaaaggca attattaaat taagagcttt agtacttaag attggttatc
     3781 cagaaaaaat cgaaaagatt tatgatcttt tgcaaattga cccagaaagg agtctttatg
     3841 aaaatgaagc tcaaatggca acggtacgca ccaagtatat gctcgataaa ttaactcagc
     3901 cagtagatcg ctcagtatgg ctcatgccag gaaacttgaa caatgcttgt tatgatccac
     3961 aaagaaatga tttaactttc ccagctggta ttttgcaagc gccattttat gatattcatc
     4021 aatcccgtgg tgcaaattac ggtggtatcg gtgcaactat tggtcatgaa gtttctcatg
     4081 cctttgataa tagtggtgct aaatttgatg aacacggaaa tatgaataac tggtggactg
     4141 atgaagactt cgctgaattc aataagcggg ttggccaaat ggttgatatt tttgatggct
     4201 tgcaatacgg tccagctaag attaacggta agcaagtagt aggagaaaat attgctgact
     4261 tggcagggct tgcttgtgct gttcaagctg gtaagaacga caatgttgat ttgaaagact
     4321 tgtttgaaaa ttatgcaaga agctggatgc aaaagcaacg tccagaagca attaaaactg
     4381 aagtgcaagt tgacgttcat gcaccacaac caactcgtgt aaatatccca gttcaatgtc
     4441 aagacgattt ctacactgca tttgatgtta agcctgatga tggcatgtgg cttgatcctg
     4501 aagatagaat tactatttgg taaatcgata gcccgcctaa tgagcgggct tttttttgat
     4561 cccccaaaga agcgcgtaat atcaggttta agagacaata agaaaaaaag attgaaaaaa
     4621 gtgacattaa attcttgaca gggagagata ggtttgatag aatataatag gtttgataga
     4681 atataatagg gtaccgagct cgaattcctg cagcccaaat catgaaaaaa aagattatct
     4741 cagctatttt aatgtctaca gtgatacttt ctgctgcagc cccgttgtca ggtgtttacg
     4801 ctatggcgat tccaacaaga agcgaagtcc cagaagattt gaagtgggat ttaacccgca
     4861 tctttaaaac agaccaagac tgggaaaatg cctttgacaa ggcaaaagat gacgtggcta
     4921 agctaagtga attaaaggga agcttggcta aatcaggcaa agatttgtat gaaggcttga
     4981 ccaagatttt ggcagttaaa cgtgatgtag aaaatattta cgtttatgcc actatgtcta
     5041 gcgatgttga tacttctaac tcacattatt tgggctacgt tagccgcgtg caaagcttgt
     5101 ccaatcaatt tgaagcaaca accagtttta ttaatcctga aattttgagt atccctgccg
     5161 aaaagtttga acaattcaaa aaagacgagc caagattagc tgattacgcc cactatttgg
     5221 aaatgatcac taacaagcgt cctcatactt tgccagcaga agaagaaaaa attatcgctg
     5281 acgcagggga tgctatgagc gtgtcagaga atacctttaa cgttttaacc aactctgaca
     5341 tggaatatgg ttatgtgcaa gatgaagacg gcaacatgga gcaattatcc aatggcttgt
     5401 attcattatt gattcagtcc caaaatcgtg acgtgcgtaa aggtgctttt aatactctct
     5461 atgccagcta tggtcaattc caaaactcgc ttgcctctac tctctccggc gttgtgaaaa
     5521 aacataacta caacgcacgc atgcacaagt atgattcagc tcgtgaagcc gcattagctg
     5581 ataacggcgt acctgttgaa gtttacgaca cattaattaa agaagttgat tcacaccttg
     5641 acttgcttca ccgttatgtc gcattgcgca agaaaatttt aggtcttaaa gacttacaaa
     5701 tgtgggacat gtacgtgccg ctaactggta agcctgcttt gtcttacaac tttgaagagg
     5761 ctaaaaaggt agctaaagaa gccatgaagc cacttggcga agactactta aagcatgttg
     5821 attatatttt taacaaccgt gtgattgatc ctgttgaatc taagggcaag gttactggtg
     5881 cttactctgg tggtgcttac gataccgatc catatgaact tttgaactgg gaagacaata
     5941 tcgattcact ctatacttta gttcatgaaa ctggacactc agttcactct tggtacaccc
     6001 gccacagtca gccttatatc tatggtaatt acccaatctt cgtggctgaa attgcttcaa
     6061 ccactaatga aaatattttg actgaatatt tcttggacca tatcactgat cctaagacgc
     6121 gcgcattcat cttgaaccac taccttgatt cattcaaggg tacattgttc cgccaaactc
     6181 aatttgcggt atttgaacaa tttatccacg aagcagatgc taagggcgaa ccattgactg
     6241 ccgatatttt ggatgatgtt tatggtcaaa ttaaccagca ttactacggc gacagtgttg
     6301 aacctggtgg cgatattgcg cttgaatggt cacgaattcc gcacttctac tacaacttct
     6361 acgtttatca atatgcgact ggatttgcgg ctgcaacggc tttggctaac aaggttgtgc
     6421 atggtagtca ggctgatagg gatgcatacc tgggctacct taagtcaggt tctagtgact
     6481 atcctactga gatcatgaag cgtgccggcg ttgacatgac taagcccgat tatttgaaag
     6541 atgctttcaa gacttttgaa aagagattga acgaattcga gagtttgatt ggtaagtaat
     6601 ctagagagct caagcttttc aaggcaatct gcctcctcat cctcttcatc ctcttcgtct
     6661 tggtagcttt ttaaatatgg gtcgatcgaa ttcggtcctc gggatatgat aagattaata
     6721 gttttagcta ttaatctttt tttattttta tttaagaatg gcttaataaa gcggttactt
     6781 tggatttttg tgagcttgga ctagaaaaaa acttcacaaa atgctatact aggtaggtaa
     6841 aaaaatattc ggaggaattt tgaatctttg aaccaaagga tccatgcagc gtaaaaagaa
     6901 agggctttct atcttactgg ctggaactgt agccttgggc gcccttgcgg tcctgcccgt
     6961 aggtgagatc caggcaaagg cggcgatcag tcaacagatg gctcatgaat taactgtgca
     7021 ggaacttgaa aagttttctg ctgattttaa taaaaatcct aaaaataaag tcgttgctcg
     7081 tgctgcccaa cgtagcggtg tacttgaagc ttcttataat gaccgcgttc aaagcgaatt
     7141 aacccgtgtc ttttcaactg aacttgatac tgacaacgtt actaaccaaa aacactcagg
     7201 tcgttgctgg ttatttgcca cattaaacgt tttgcgtcat gaatttggca agaaatacaa
     7261 ggcaaaagac tttactttct cacaagcata caacttcttc tgggacaaga ttgaacgtgc
     7321 taacatgttc tacaaccgta tcttagacag cgctgatatg ccacttgatt ctcgtcaagt
     7381 taagactgac ttagactttg caggtacaga tggtggtcaa ttccaaatgg ctgctgcctt
     7441 agttgaaaaa tatggtgtcg taccttcata tgctatgcct gaaaccttta acactaacaa
     7501 cactactggt tttgccactg cattaggcga caagcttaag aaggatgctt tggttcttag
     7561 aaaattaaag caagaaggca aagatgacga aatcaagaag actcgtgaaa aattcttgag
     7621 cgaagtttac caaatgactg ctattgctgt tggtgaacca cctaagaagt tcgatcttga
     7681 ataccgtgat gacgataaga agtaccactt agaaaaagac cttactccac ttgaattctt
     7741 gcacaagtac ttaggtggcg ttgactttga tgactacgtt gttttgacca acgcaccaga
     7801 ccacgaatat gacaagcttt atggtttacc agcagaagac aacgtctctg gttcaatcag
     7861 aattaaactt ttgaatgttc ctatggaata cttaaccgct gcttctattg ctcaattaaa
     7921 agacggtgaa gctgtttggt tcggtaatga tgtgcttcgt caaatggacc gtaagactgg
     7981 ctaccttgac actaaccttt acaagttgga tgacttattt ggcgttgacc ttaagatgtc
     8041 aaaggctgac agattaaaga ctggtgtcgg cgaagtttct cacgccatga ccttagtcgg
     8101 tgttgatgaa gacaacggtg aagttcgtca atggaaagtt gaaaactcat ggggcgacaa
     8161 gtccggtgca aagggttatt acgtaatgaa caatgaatgg ttcaacgatt acgtttatga
     8221 agttgtcgtt cacaagaagt atttgactga taagcaaaag aaacttgcag aaggcccaat
     8281 tactgatctt cctgcatggg attcacttgc ttaaactagt tctagagagc tcaagctttc
     8341 tttgaaccaa aattagaaaa ccaaggcttg aaacgttcaa ttgaaatggc aattaaacaa
     8401 attacagcac gtgttgcttt gattgatagc caaaaagcag cagttgataa agcaattact
     8461 gatattgctg aaaaattgta atttataaat aaaaatcacc ttttagaggt ggttttttta
     8521 tttataaatt attcgtttga tttcgctttc gatagaacaa tcaaatcgtt tctgagacgt
     8581 tttagcgttt atttcgttta gttatcggca taatcgttaa aacaggcgtt atcgtagcgt
     8641 aaaagccctt gagcgtagcg tggctttgca gcgaagatgt tgtctgttag attatgaaag
     8701 ccgatgactg aatgaaataa taagcgcagc gtccttctat ttcggttgga ggaggctcaa
     8761 gggagtttga gggaatgaaa ttccctcatg ggtttgattt taaaaattgc ttgcaatttt
     8821 gccgagcggt agcgctggaa aatttttgaa aaaaatttgg aatttggaaa aaaatggggg
     8881 gaaaggaagc gaattttgct tccgtactac gaccccccat taagtgccga gtgccaattt
     8941 ttgtgccaaa aacgctctat cccaactggc tcaagggttt gaggggtttt tcaatcgcca
     9001 acgaatcgcc aacgttttcg ccaacgtttt ttataaatct atatttaagt agctttattt
     9061 ttgtttttat gattacaaag tgatacacta attttataaa attatttgat tggagttttt
     9121 taaatggtga tttcagaatc gaaaaaaaga gttatgattt ctctgacaaa agagcaagat
     9181 aaaaaattaa cagatatggc gaaacaaaaa gatttttcaa aatctgcggt tgcggcgtta
     9241 gctatagaag aatatgcaag aaaggaatca gaacaaaaaa aataagcgaa agctcgcgtt
     9301 tttagaagga tacgagtttt cgctacttgt ttttgataag gtaattatat catggctatt
     9361 aaaaatacta aagctagaaa ttttggattt ttattatatc ctgactcaat tcctaatgat
     9421 tggaaagaaa aattagagag tttgggcgta tctatggctg tcagtccttt acacgatatg
     9481 gacgaaaaaa aagataaaga tacatggaat agtagtgatg ttatacgaaa tggaaagcac
     9541 tataaaaaac cacactatca cgttatatat attgcacgaa atcctgtaac aatagaaagc
     9601 gttaggaaca agattaagcg aaaattgggg aatagttcag ttgctcatgt tgagatactt
     9661 gattatatca aaggttcata tgaatatttg actcatgaat caaaggacgc tattgctaag
     9721 aataaacata tatacgacaa aaaagatatt ttgaacatta atgattttga tattgaccgc
     9781 tatataacac ttgatgaaag ccaaaaaaga gaattgaaga atttactttt agatatagtg
     9841 gatgactata atttggtaaa tacaaaagat ttaatggctt ttattcgcct taggggagcg
     9901 gagtttggaa ttttaaatac gaatgatgta aaagatattg tttcaacaaa ctctagcgcc
     9961 tttagattat ggtttgaggg caattatcag tgtggatata gagcaagtta tgcaaaggtt
    10021 cttgatgctg aaacggggga aataaaatga caaacaaaga aaaagagtta tttgctgaaa
    10081 atgaggaatt aaaaaaagaa attaaggact taaaagagcg tattgaaaga tacagagaaa
    10141 tggaagttga attaagtaca acaatagatt tattgagagg agggattatt gaataaataa
    10201 aagcccccct gacgaaagtc gacggcaata gttaccctta ttatcaagat aagaaagaaa
    10261 aggatttttc gctacgctca aatcctttaa aaaaacacaa aagaccacat tttttaatgt
    10321 ggtcttttat tcttcaacta aagcacccat tagttcaaca aacgaaaatt ggataaagtg
    10381 ggatattttt aaaatatata tttatgttac agtaatattg acttttaaaa aaggattgat
    10441 tctaatgaag aaagcagaca agtaagcctc ctaaattcac tttagataaa aatttaggag
    10501 gcatatcaaa tgaactttaa taaaattgat ttagacaatt ggaagagaaa agagatattt
    10561 aatcattatt tgaaccaaca aacgactttt agtataacca cagaaattga tattagtgtt
    10621 ttataccgaa acataaaaca agaaggatat aaattttacc ctgcatttat tttcttagtg
    10681 acaagggtga taaactcaaa tacagctttt agaactggtt acaatagcga cggagagtta
    10741 ggttattggg ataagttaga gccactttat acaatttttg atggtgtatc taaaacattc
    10801 tctggtattt ggactcctgt aaagaatgac ttcaaagagt tttatgattt atacctttct
    10861 gatgtagaga aatataatgg ttcggggaaa ttgtttccca aaacacctat acctgaaaat
    10921 gctttttctc tttctattat tccttggact tcatttactg ggtttaactt aaatatcaat
    10981 aataatagta attaccttct acccattatt acagcaggaa aattcattaa taaaggtaat
    11041 tcaatatatt taccgctatc tttacaggta catcattctg tttgtgatgg ttatcatgct
    11101 ggattgttta tgaactctat tcaggaattg tcagataggc ctaatgactg gcttttataa
    11161 tatgagataa tgccgactgt actttttaca gtcggttttc taatgtcact aacctgcccc
    11221 gttagttgaa gaaggttttt atattacagc tccagatctg gatccgatat ctagatgcat
    11281 tcgcgaggta ccgagctcga attcactggc cgtcgtttta caacgtcgtg actgggaaaa
    11341 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
    11401 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
    11461 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
    11521 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
    11581 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
    11641 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcga
//
