LOCUS       V014063                 4296 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014063
VERSION     V014063
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4296
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label="AmpR promoter"
     CDS             201..1058
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1232..1820
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    2108..2129
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2144..2174
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             2277..3863
                     /gene="hly"
                     /label="Listeriolysin O"
                     /note="Listeriolysin O from Listeria monocytogenes serotype
                     1/2a (strain 08-5578). Accession#: D2NZ73"
     primer_bind     complement(3902..3918)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccaagctt gcatgcaggc ctctgcagtc gacgggcccg ggatccatga
     2281 aaaaaataat gctagttttt attacactta tattagttag tctaccaatt gcgcaacaaa
     2341 ctgaagcaaa ggatgcatct gcattcaata aagaaaattc aatttcatcc atggcaccac
     2401 cagcatctcc gcctgcaagt cctaagacgc caatcgaaaa gaaacacgcg gatgaaatcg
     2461 ataagtatat acaaggattg gattacaata aaaacaatgt attagtatac cacggagatg
     2521 cagtgacaaa tgtgccgcca agaaaaggtt acaaagatgg aaatgaatat attgttgtgg
     2581 agaaaaagaa gaaatccatc aatcaaaata atgcagacat tcaagttgtg aatgcaattt
     2641 cgagcctaac ctatccaggt gctctcgtaa aagcgaattc ggaattagta gaaaatcaac
     2701 cagatgttct ccctgtaaaa cgtgattcat taacactcag cattgatttg ccaggtatga
     2761 ctaatcaaga caataaaatc gttgtaaaaa atgccactaa atcaaacgtt aacaacgcag
     2821 taaatacatt agtggaaaga tggaatgaaa aatatgctca agcttatcca aatgtaagtg
     2881 caaaaattga ttatgatgac gaaatggctt acagtgaatc acaattaatt gcgaaatttg
     2941 gtacagcatt taaagctgta aataatagct tgaatgtaaa cttcggcgca atcagtgaag
     3001 ggaaaatgca agaagaagtc attagtttta aacaaattta ctataacgtg aatgttaatg
     3061 aacctacaag accttccaga tttttcggca aagctgttac taaagagcag ttgcaagcgc
     3121 ttggagtgaa tgcagaaaat cctcctgcat atatctcaag tgtggcgtat ggccgtcaag
     3181 tttatttgaa attatcaact aattcccata gtactaaagt aaaagctgct tttgatgctg
     3241 ccgtaagcgg aaaatctgtc tcaggtgatg tagaactaac aaatatcatc aaaaattctt
     3301 ccttcaaagc cgtaatttac ggaggttccg caaaagatga agttcaaatc atcgacggca
     3361 acctcggaga cttacgcgat attttgaaaa aaggcgctac ttttaatcga gaaacaccag
     3421 gagttcccat tgcttataca acaaacttcc taaaagacaa tgaattagct gttattaaaa
     3481 acaactcaga atatattgaa acaacttcaa aagcttatac agatggaaaa attaacatcg
     3541 atcactctgg aggatacgtt gctcaattca acatttcttg ggatgaagta aattatgatc
     3601 ctgaaggtaa cgaaattgtt caacataaaa actggagcga aaacaataaa agcaagctag
     3661 ctcatttcac atcgtccatc tatttgccag gtaacgcgag aaatattaat gtttacgcta
     3721 aagaatgcac tggtttagct tgggaatggt ggagaacggt aattgatgac cggaacttac
     3781 cacttgtgaa aaatagaaat atctccatct ggggcaccac gctttatccg aaatatagta
     3841 ataaagtaga taatccaatc gaataatcta gatgcattcg cgaggtaccg agctcgaatt
     3901 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     3961 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     4021 gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc
     4081 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
     4141 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     4201 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     4261 gtcagaggtt ttcaccgtca tcaccgaaac gcgcga
//
