LOCUS       40924_45178        3548 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Fluorescent tagging vector pUC57-Ps12-tag-CFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Norris MH, Kang Y, Hoang TT.
  TITLE     Stable site-specific fluorescent tagging tools optimized for 
            burkholderia species
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Norris MH, Kang Y, Hoang TT.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-APR-2010) Microbiology, University of Hawaii at Manoa,
            2538 Mccarthy Mall Snyder 310, Honolulu, HI 96822, USA
REFERENCE   3  (bases 1 to 3548)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3548)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-APR-2010) Microbiology, University of Hawaii at Manoa, 2538 
            Mccarthy Mall Snyder 310, Honolulu, HI 96822, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3548
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             523..1236
                     /codon_start=1
                     /label=TagCFP
                     /note="monomeric cyan fluorescent protein"
                     /translation="MSGGEELFAGIVPVLIELDGDVHGHKFSVRGEGEGDADYGKLEIK
                     FICTTGKLPVPWPTLVTTLAWGIQCFARYPEHMKMNDFFKSAMPEGYIQERTIHFQDDG
                     KYKTRGEVKFEGDTLVNRVELKGEGFKEDGNILGHKLEYSAISDNVYIMPDKANNGLEA
                     NFKIRHNIEGGGVQLADHYQTNVPLGDGPVLIPINHYLSCQSAISKDRNEARDHMVLLE
                     SFSAYCHTHGMDELYR"
     primer_bind     complement(1327..1343)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1351..1367)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1375..1405)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1420..1441)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1729..2317)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2491..3348)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3349..3453)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga tccaattcta gaagctgttg actcgcttgg gattttcgga atatcatgcc
      481 gggttccggg accatgggat cgagcttcga aaggacaagc atatgagcgg gggcgaggag
      541 ctgttcgctg gcatcgtgcc cgtgctgatc gagctggacg gcgacgtgca cggccacaag
      601 ttcagcgtgc gcggtgaggg agagggcgac gccgactacg gcaagctgga gatcaagttc
      661 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tggtgaccac cctcgcctgg
      721 ggcatccagt gcttcgcccg ctaccccgag cacatgaaga tgaacgactt cttcaagagc
      781 gccatgcccg agggctacat ccaggagcgc accatccact tccaggacga cggcaagtac
      841 aagacccgcg gcgaggtgaa gttcgagggc gacaccctgg tgaaccgcgt cgagctgaag
      901 ggcgagggct tcaaggagga cggcaacatc ctgggccaca agttggagta cagcgccatc
      961 agcgacaacg tgtacatcat gcccgacaag gccaacaacg gcctggaggc gaacttcaag
     1021 atccgccaca acatcgaggg cggcggcgtg cagctggccg accactacca gaccaacgtg
     1081 cccctgggcg atggccccgt gctgatcccc atcaaccact acctgagctg ccagtccgcc
     1141 atcagcaagg accgcaacga agcccgcgac cacatggtgc tcctggagtc cttcagcgcc
     1201 tactgccaca cccacggcat ggacgagctg taccgctaat ttaagaagga gatataccat
     1261 ggaaattgga tcggatcccg ggcccgtcga ctgcagaggc ctgcatgcaa gcttggcgta
     1321 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     1381 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     1441 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     1501 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     1561 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     1621 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     1681 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     1741 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     1801 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     1861 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     1921 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     1981 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     2041 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     2101 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     2161 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     2221 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     2281 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     2341 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     2401 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     2461 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     2521 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     2581 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     2641 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     2701 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     2761 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     2821 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     2881 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     2941 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     3001 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     3061 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     3121 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     3181 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     3241 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     3301 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     3361 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     3421 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     3481 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     3541 ctttcgtc
//
