LOCUS       40924_45188        3563 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Fluorescent tagging vector pUC57-PS12-turbo-yfp, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Norris MH, Kang Y, Hoang TT.
  TITLE     Stable site-specific fluorescent tagging tools optimized for 
            burkholderia species
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Norris MH, Kang Y, Hoang TT.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-APR-2010) Microbiology, University of Hawaii at Manoa,
            2538 Mccarthy Mall Snyder 310, Honolulu, HI 96822, USA
REFERENCE   3  (bases 1 to 3563)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3563)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-APR-2010) Microbiology, University of Hawaii at Manoa, 2538 
            Mccarthy Mall Snyder 310, Honolulu, HI 96822, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3563
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             523..1251
                     /codon_start=1
                     /label=TurboYFP
                     /note="enhanced derivative of PhiYFP"
                     /translation="MSSGALLFHGKIPYVVEMEGNVDGHTFSIRGKGYGDASVGKVDAQ
                     FICTTGDVPVPWSTLVTTLTYGAQCFAKYGPELKDFYKSCMPDGYVQERTITFEGDGNF
                     KTRAEVTFENGSVYNRVKLNGQGFKKDGHVLGKNLEFNFTPHCLYIWGDQANHGLKSAF
                     KICHEITGSKGDFIVADHTQMNTPIGGGPVHVPEYHHMSYHVKLSKDVTDHRDNMSLKE
                     TVRAVDCRKTYDFDAGSGDTS"
     primer_bind     complement(1342..1358)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1366..1382)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1390..1420)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1435..1456)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1744..2332)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2506..3363)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3364..3468)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga tccaattcta gaagctgttg actcgcttgg gattttcgga atatcatgcc
      481 gggttccggg accatgggat cgagcttcga aaggacaagc atatgagcag cggcgccctg
      541 ctgttccacg gcaagatccc ctacgtggtg gagatggagg gcaacgtgga cggccacacc
      601 ttcagcatcc gcggcaaggg ctacggcgac gccagcgtgg gcaaggtgga cgcccagttc
      661 atctgcacca ccggcgatgt gcccgtgccc tggagcaccc tggtgaccac cctgacctac
      721 ggcgcccagt gcttcgccaa gtacggcccc gagctgaagg acttctacaa gagctgcatg
      781 cccgacggct acgtgcagga gcgcaccatc accttcgagg gcgatggcaa cttcaagacc
      841 cgcgccgagg tgaccttcga gaacggcagc gtgtacaacc gcgtgaagct gaacggccag
      901 ggcttcaaga aggatggcca cgtgctgggc aagaacctgg agttcaattt caccccccac
      961 tgcctgtaca tctggggcga ccaggccaac cacggcctga agagcgcctt caagatttgc
     1021 cacgagatca ccggcagcaa gggcgacttc atcgtggccg atcacaccca gatgaacacc
     1081 cccatcggcg gcggccccgt gcacgtgccc gagtaccacc acatgagcta ccacgtgaag
     1141 ctgagcaagg acgtgaccga ccaccgcgac aacatgagcc tgaaggagac cgtgcgcgcc
     1201 gtggactgcc gcaagaccta cgacttcgac gccggttccg gagacaccag ctgatttaag
     1261 aaggagatat accatggaaa ttggatcgga tcccgggccc gtcgactgca gaggcctgca
     1321 tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     1381 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     1441 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     1501 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     1561 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     1621 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     1681 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     1741 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     1801 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     1861 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     1921 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     1981 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     2041 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     2101 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     2161 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     2221 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     2281 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     2341 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     2401 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     2461 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     2521 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     2581 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     2641 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     2701 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     2761 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     2821 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     2881 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     2941 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     3001 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     3061 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     3121 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     3181 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     3241 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     3301 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     3361 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     3421 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     3481 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     3541 gcgtatcacg aggccctttc gtc
//
