LOCUS       V013254                 3086 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013254
VERSION     V013254
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3086
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             447..809
                     /note="ORF8 protein from Severe acute respiratory syndrome
                     coronavirus 2. Accession#: P0DTC8"
                     /label="ORF8 protein"
     primer_bind     complement(865..881)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(889..905)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(913..943)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(958..979)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1267..1855)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2029..2886)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2887..2991)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga tatcggatcc cgacacatga aatttcttgt tttcttagga atcatcacaa
      481 ctgtagctgc atttcaccaa gaatgtagtt tacagtcatg tactcaacat caaccatatg
      541 tagttgatga cccgtgtcct attcacttct attctaaatg gtatattaga gtaggagcta
      601 gaaaatcagc acctttaatt gaattgtgcg tggatgaggc tggttctaaa tcacccattc
      661 agtacatcga tatcggtaat tatacagttt cctgtttacc ttttacaatt aattgccagg
      721 aacctaaatt gggtagtctt gtagtgcgtt gttcgttcta tgaagacttt ttagagtatc
      781 atgacgttcg tgttgtttta gatttcatct aagtgtcggg cccgtcgact gcagaggcct
      841 gcatgcaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
      901 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
      961 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     1021 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     1081 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     1141 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     1201 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     1261 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     1321 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     1381 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     1441 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     1501 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     1561 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     1621 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     1681 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     1741 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     1801 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     1861 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     1921 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     1981 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     2041 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     2101 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     2161 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     2221 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     2281 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     2341 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     2401 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     2461 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     2521 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     2581 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     2641 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     2701 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     2761 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     2821 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     2881 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     2941 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     3001 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     3061 taggcgtatc acgaggccct ttcgtc
//
