LOCUS       V013754                 3733 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013754
VERSION     V013754
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3733
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label="AmpR promoter"
     CDS             201..1058
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1232..1820
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    2108..2129
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2144..2174
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             2277..3542
                     /gene="N"
                     /label="Nucleoprotein"
                     /note="Nucleoprotein from Severe acute respiratory syndrome
                     coronavirus. Accession#: P59595"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccaagctt gcatgcaggc ctctgcagtc gacgggcccg ggatccatgt
     2281 ctgataatgg accccaatca aaccaacgta gtgccccccg cattacattt ggtggaccca
     2341 cagattcaac tgacaataac cagaatggag gacgcaatgg ggcaaggcca aaacagcgcc
     2401 gaccccaagg tttacccaat aatactgcgt cttggttcac agctctcact cagcatggca
     2461 aggaggaact tagattccct cgaggccagg gcgttccaat caacaccaat agtggtccag
     2521 atgaccaaat tggctactac cgaagagcta cccgacgagt tcgtggtggt gacggcaaaa
     2581 tgaaagagct cagccccaga tggtacttct attacctagg aactggccca gaagcttcac
     2641 ttccctacgg cgctaacaaa gaaggcatcg tatgggttgc aactgaggga gccttgaata
     2701 cacccaaaga ccacattggc acccgcaatc ctaataacaa tgctgccacc gtgctacaac
     2761 ttcctcaagg aacaacattg ccaaaaggct tctacgcaga gggaagcaga ggcggcagtc
     2821 aagcctcttc tcgctcctca tcacgtagtc gcggtaattc aagaaattca actcctggca
     2881 gcagtagggg aaattctcct gctcgaatgg ctagcggagg tggtgaaact gccctcgcgc
     2941 tattgctgct agacagattg aaccagcttg agagcaaagt ttctggtaaa ggccaacaac
     3001 aacaaggcca aactgtcact aagaaatctg ctgctgaggc atctaaaaag cctcgccaaa
     3061 aacgtactgc cacaaaacag tacaacgtca ctcaagcatt tgggagacgt ggtccagaac
     3121 aaacccaagg aaatttcggg gaccaagacc taatcagaca aggaactgat tacaaacatt
     3181 ggccgcaaat tgcacaattt gctccaagtg cctctgcatt ctttggaatg tcacgcattg
     3241 gcatggaagt cacaccttcg ggaacatggc tgacttatca tggagccatt aaattggatg
     3301 acaaagatcc acaattcaaa gacaacgtca tactgctgaa caagcacatt gacgcataca
     3361 aaacattccc accaacagag cctaaaaagg acaaaaagaa aaagactgat gaagctcagc
     3421 ctttgccgca gagacaaaag aagcagccca ctgtgactct tcttcctgcg gctgacatgg
     3481 atgatttctc cagacaactt caaaattcca tgagtggagc ttctgctgat tcaactcagg
     3541 cataacatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     3601 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     3661 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     3721 ccgaaacgcg cga
//
