LOCUS       V013757                 3706 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013757
VERSION     V013757
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3706
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label="AmpR promoter"
     CDS             201..1058
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1232..1820
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    2108..2129
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2144..2174
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             2277..3467
                     /gene="N"
                     /label="Nucleoprotein"
                     /note="Nucleoprotein from Middle East respiratory
                     syndrome-related coronavirus (isolate United
                     Kingdom/H123990006/2012). Accession#: K9N4V7"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccaagctt gcatgcaggc ctctgcagtc gacgggcccg ggatccatgg
     2281 catcccctgc tgcacctcgt gctgtttcct ttgccgataa caatgatata acaaatacaa
     2341 acctatctcg aggtagagga cgtaatccaa aaccacgagc tgcaccaaat aacactgtct
     2401 cttggtacac tgggcttacc caacacggga aagtccctct tacctttcca cctgggcagg
     2461 gtgtacctct taatgccaat tctacccctg cgcaaaatgc tgggtattgg cggagacagg
     2521 acagaaaaat taataccggg aatggaatta agcaactggc tcccaggtgg tacttctact
     2581 acactggaac tggacccgaa gcagcactcc cattccgggc tgttaaggat ggcatcgttt
     2641 gggtccatga agatggcgcc actgatgctc cttcaacttt tgggacgcgg aaccctaaca
     2701 atgattcagc tattgttaca caattcgcgc ccggtactaa gcttcctaaa aacttccaca
     2761 ttgaggggac tggaggcaat agtcaatcat cttcaagagc ctctagctta agcagaaact
     2821 cttccagatc tagttcacaa ggttcaagat caggaaactc tacccgcggc acttctccag
     2881 gtccatctgg aatcggagca gtaggaggtg atctacttta ccttgatctt ctgaacagac
     2941 tacaagccct tgagtctggc aaagtaaagc aatcgcagcc aaaagtaatc actaagaaag
     3001 atgctgctgc tgctaaaaat aagatgcgcc acaagcgcac ttccaccaaa agtttcaaca
     3061 tggtgcaagc ttttggtctt cgcggaccag gagacctcca gggaaacttt ggtgatcttc
     3121 aattgaataa actcggcact gaggacccac gttggcccca aattgctgag cttgctccta
     3181 cagccagtgc ttttatgggt atgtcgcaat ttaaacttac ccatcagaac aatgatgatc
     3241 atggcaaccc tgtgtacttc cttcggtaca gtggagccat taaacttgac ccaaagaatc
     3301 ccaactacaa taagtggttg gagcttcttg agcaaaatat tgatgcctac aaaaccttcc
     3361 ctaagaagga aaagaaacaa aaggcaccaa aagaagaatc aacagaccaa atgtctgaac
     3421 ctccaaagga gcagcgtgtg caaggtagca tcactcagcg cactcgcacc cgtccaagtg
     3481 ttcagcctgg tccaatgatt gatgttaaca ctgattagca tatggtgcac tctcagtaca
     3541 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     3601 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     3661 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcga
//
