LOCUS       V013756                 3811 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013756
VERSION     V013756
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label="AmpR promoter"
     CDS             201..1058
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1232..1820
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    2108..2129
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2144..2174
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             2277..3620
                     /gene="N"
                     /label="Nucleoprotein"
                     /note="Nucleoprotein from Human coronavirus OC43.
                     Accession#: P33469"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccaagctt gcatgcaggc ctctgcagtc gacgggcccg ggatccatgt
     2281 cttttactcc tggtaagcaa tccagtagta gagcgtcctc tggaaatcgt tctggtaatg
     2341 gcatcctcaa gtgggccgat cagtccgacc agtttagaaa tgttcaaacc aggggtagaa
     2401 gagctcaacc caagcaaact gctacctctc agcaaccatc aggagggaat gttgtaccct
     2461 actattcttg gttctctgga attactcagt ttcaaaaggg aaaggagttt gagtttgtag
     2521 aaggacaagg tgtgcctatt gcaccaggag tcccagctac tgaagctaag gggtactggt
     2581 acagacacaa cagacgttct tttaaaacag ccgatggcaa ccagcgtcaa ctgctgccac
     2641 gatggtattt ttactatctg ggaacaggac cgcatgctaa agaccagtac ggcaccgata
     2701 ttgacggagt ctactgggtc gctagcaacc aggctgatgt caataccccg gctgacattg
     2761 tcgatcggga cccaagtagc gatgaggcta ttccgactag gtttccgcct ggcacggtac
     2821 tccctcaggg ttactatatt gaaggctcag gaaggtctgc tcctaattcc agatctactt
     2881 cgcgcacatc cagcagagcc tctagtgcag gatcgcgtag tagagccaat tctggcaata
     2941 gaacccctac ctctggtgta acacctgaca tggctgatca aattgctagt cttgttctgg
     3001 caaaacttgg caaggatgcc actaaacctc agcaagtaac taagcatact gccaaagaag
     3061 tcagacagaa aattttgaat aagccccgcc agaagaggag ccccaataaa caatgcactg
     3121 ttcagcagtg ttttggtaag agaggcccta atcagaattt tggtggtgga gaaatgttaa
     3181 aacttggaac tagtgaccca cagttcccca ttcttgcaga actcgcaccc acagctggtg
     3241 cgtttttctt tggatcaaga ttagagttgg ccaaagtgca gaatttatct gggaatcctg
     3301 acgagcccca gaaggatgtt tatgaattgc gctataacgg cgcaattagg tttgacagta
     3361 cactttcagg ttttgagacc ataatgaagg tgctgaatga gaatttgaat gcctatcaac
     3421 aacaagatgg tatgatgaat atgagtccaa aaccacagcg tcagcgtggt cataagaatg
     3481 gacaaggaga aaatgataat ataagtgttg cagtgcccaa aagccgcgtg cagcaaaata
     3541 agagtagaga gttgactgca gaggacatca gccttcttaa gaagatggat gagccctata
     3601 ctgaagacac ctcagaaata taacatatgg tgcactctca gtacaatctg ctctgatgcc
     3661 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     3721 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     3781 aggttttcac cgtcatcacc gaaacgcgcg a
//
