LOCUS       V013755                 3790 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013755
VERSION     V013755
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label="AmpR promoter"
     CDS             201..1058
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1232..1820
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    2108..2129
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2144..2174
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             2277..3599
                     /gene="N"
                     /label="Nucleoprotein"
                     /note="Nucleoprotein from Human coronavirus HKU1 (isolate
                     N1). Accession#: Q5MQC6"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccaagctt gcatgcaggc ctctgcagtc gacgggcccg ggatccatgt
     2281 cttatactcc cggtcattat gctggaagta gaagctcctc tggaaatcgt tcaggaatcc
     2341 tcaagaaaac ttcttgggct gaccaatctg agcgaaatta ccaaaccttt aatagaggca
     2401 gaaaaaccca acctaaattc actgtgtcta ctcaaccaca aggaaatact atcccacatt
     2461 attcctggtt ctccgggatc actcaatttc aaaaaggtag agactttaaa ttttcagatg
     2521 gtcaaggagt tcccattgct ttcggagtac ccccttctga agcaaaagga tattggtata
     2581 gacacagccg gcgttctttt aaaacagctg atggtcaaca aaagcagttg ttaccgagat
     2641 ggtatttcta ctatctcggt accggcccat atgccaatgc atcctatggt gaatccctcg
     2701 aaggggtctt ctgggttgct aatcaccaag ctgacacttc tactccctcc gatgtttcgt
     2761 caagggatcc tactactcaa gaagctatcc ctactaggtt tccgcctggt acgattttgc
     2821 ctcaaggcta ttatgttgaa ggctcaggaa ggtctgcttc taatagtcga ccaggttcac
     2881 gttctcaatc acgtggaccc aataatcgtt cattaagtag aagtaattct aattttagac
     2941 attcagattc tatagtaaaa cctgatatgg ctgatgagat cgctaatctt gttttagcca
     3001 agcttggtaa agattctaaa cctcagcaag tcactaagca aaatgccaag gaaatcaggc
     3061 ataaaatttt aacaaaacct cgccaaaagc gaactcctaa taaacattgt aatgttcaac
     3121 agtgttttgg taaaagagga ccttctcaaa attttggtaa tgctgaaatg ttaaagcttg
     3181 gtactaatga tcctcagttt cctattcttg cagaattagc tcctacacca ggtgcttttt
     3241 tctttggttc taaattagac ttggttaaaa gagattccga ggctgactca cctgttaaag
     3301 atgtttttga acttcattat tctggttcta ttaggtttga tagtacttta ccaggctttg
     3361 agacaattat gaaagttctt gaagagaatt taaatgctta cgttaattct aatcagaaca
     3421 ctgattctga ttcgttgagt tctaaacctc agcgtaaaag aggtgttaaa caattaccag
     3481 aacagtttga ctctcttaat ttaagtgctg gtactcagca catttcaaat gattttactc
     3541 ctgaggatca tagtttactt gctactcttg atgatcctta tgtagaagac tctgttgctt
     3601 aacatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     3661 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     3721 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     3781 aaacgcgcga
//
