LOCUS       V013753                 3634 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013753
VERSION     V013753
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3634
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label="AmpR promoter"
     CDS             201..1058
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1232..1820
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    2108..2129
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2144..2174
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             2277..3443
                     /gene="N"
                     /label="Nucleoprotein"
                     /note="Nucleoprotein from Human coronavirus 229E.
                     Accession#: P15130"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccaagctt gcatgcaggc ctctgcagtc gacgggcccg ggatccatgg
     2281 ctacagtcaa atgggctgat gcatctgaac cacaacgtgg tcgtcagggt agaatacctt
     2341 attctcttta tagccctttg cttgttgata gtgaacaacc ttggaaggtg atacctcgta
     2401 atttggtacc catcaacaag aaagacaaaa ataagcttat aggctattgg aatgttcaaa
     2461 aacgtttcag aactagaaag ggcaaacggg tggatttgtc acccaagctg catttttatt
     2521 atcttggcac aggaccccat aaagatgcaa aatttagaga gcgtgttgaa ggtgtcgtct
     2581 gggttgctgt tgatggtgct aaaactgaac ctacaggtta cggtgttagg cgcaagaatt
     2641 cagaaccaga gataccacac ttcaatcaaa agctcccaaa tggtgttact gttgttgaag
     2701 aacctgactc ccgtgctcct tcccggtctc agtcgaggtc gcagagtcgc ggtcgtggtg
     2761 aatccaaacc tcaatctcgg aatccttcaa gtgacagaaa ccataacagt caggatgaca
     2821 tcatgaaggc agttgctgcg gctcttaaat ctttaggttt tgacaagcct caggaaaaag
     2881 ataaaaagtc agcgaaaacg ggtactccta agccttctcg taatcagagt cctgcttctt
     2941 ctcaaacttc tgccaagagt cttgctcgtt ctcagagttc tgaaacaaaa gaacaaaagc
     3001 atgaaatgca aaagccacgg tggaaaagac agcctaatga tgatgtgaca tctaatgtca
     3061 cacaatgttt tggccccaga gaccttgacc acaactttgg aagtgcaggt gttgtggcca
     3121 atggtgttaa agctaaaggc tatccacaat ttgctgagct tgtgccgtca acagctgcta
     3181 tgctgtttga tagtcacatt gtttccaaag agtcaggcaa cactgtggtc ttgactttca
     3241 ctactagagt gactgtgccc aaagaccatc cacacttggg taagtttctt gaggagttaa
     3301 atgcattcac tagagaaatg caacaacatc ctcttcttaa ccctagtgca ctagaattca
     3361 acccatctca aacttcacct gcaactgctg aaccagtgcg tgatgaagtt tctattgaaa
     3421 ctgacataat tgatgaagta aactaacata tggtgcactc tcagtacaat ctgctctgat
     3481 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     3541 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     3601 cagaggtttt caccgtcatc accgaaacgc gcga
//
