LOCUS       Exported                1835 bp DNA     circular SYN 11-SEP-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   11111111
  TITLE     Direct Submission
  JOURNAL   Exported Sep 11, 2025 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1835
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          join(344..1835,1..343)
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        375..479
                     /gene="bla"
                     /label=AmpR promoter
     CDS             480..1340
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      join(1511..1835,1..264)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
       61 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
      121 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
      181 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
      241 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
      301 ttttgctggc cttttgctca catgttcttt ccgatatcat tgggacgtca ggtggcactt
      361 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
      421 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
      481 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
      541 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
      601 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
      661 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
      721 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
      781 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
      841 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
      901 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
      961 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     1021 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     1081 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     1141 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     1201 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     1261 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     1321 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     1381 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     1441 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     1501 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     1561 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     1621 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     1681 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     1741 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     1801 taccggataa ggcgcagcgg tcgggctgaa cgggg
//
