LOCUS       Exported                2579 bp DNA     circular SYN 30-SEP-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pUC57-Kan
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2579)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 30, 2025 from SnapGene 6.0.2
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2579
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          join(749..2579,1..748)
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    146..167
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        182..212
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    220..236
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     244..260
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             256..603
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHAGLCSRRARDPISRCIREVPSSNSLAVVLQRRDWEN
                     PGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIW
                     CTLSTICSDAA"
     CDS             862..1671
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      1849..2437
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
       61 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
      121 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
      181 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
      241 acacaggaaa cagctatgac catgattaca ccaagcttgc atgcaggcct ctgcagtcga
      301 cgggcccggg atccgatatc tagatgtatt cgcgaggtac cgagctcgaa ttctctggcc
      361 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
      421 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
      481 caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat
      541 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca
      601 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
      661 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
      721 ttttcaccgt catcaccgaa acgcgcgatg cagctctggc ccgtgtctca aaatctctga
      781 tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata
      841 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta
      901 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
      961 tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa
     1021 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
     1081 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
     1141 ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat
     1201 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
     1261 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
     1321 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
     1381 gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact
     1441 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
     1501 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
     1561 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     1621 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg
     1681 gttaattggt tgtaacatta ttcagattgg gcttgattta aaacttcatt tttaatttaa
     1741 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     1801 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     1861 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     1921 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     1981 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     2041 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     2101 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     2161 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     2221 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     2281 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     2341 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     2401 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     2461 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     2521 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaa
//
