LOCUS       62056_22185        4509 bp DNA     circular SYN 13-JUN-2020
DEFINITION  Expression vector pUC57-Kan-mcr-7 DNA, complete seuence.
ACCESSION   LC548751
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Suzuki M.
  TITLE     Bacterial ARG expression libraries
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4509)
  AUTHORS   Suzuki M.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) Contact:Masato Suzuki National Institute of 
            Infectious Diseases, AMR Research Center; 4-2-1 Aobacho, 
            Higashimurayama, Tokyo 189-0002, Japan
REFERENCE   3  (bases 1 to 4509)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-MAY-2020) Contact:Masato Suzuki National Institute of Infectious
            Diseases, AMR Research Center; 4-2-1 Aobacho, Higashimurayama, Tokyo
            189-0002, Japan"
FEATURES             Location/Qualifiers
     source          1..4509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        466..494
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     gene            742..2361
                     /gene="mcr-7"
                     /label=mcr-7
     CDS             742..2361
                     /codon_start=1
                     /transl_table=11
                     /gene="mcr-7"
                     /product="lipid A phosphoethanolamine transferase MCR-7"
                     /label=mcr-7
                     /protein_id="BCG50373.1"
                     /translation="MRITLGVMKVNLLLVLFFALVLNWPFFLRFYSVISGLEHVRAGFV
                     ISVPLVLLAALNAVFIPFTFRWLLKPFFSLLILTGSIVSYAMLKYGVIFDASMIQNIVE
                     TNNSEATSYLNVPVVLWFLLTGVLPMVVLWSLKVRYPANWYKGLAIRAGALAFSLLFVG
                     GVAALYYQDYVSIGRNHRILGKQIVPANYVNGIYKYARDVVFATPIPYQPLGTDAKVVA
                     KGDKPTLMFLVVGETARGKNFSMNGYEKETNPFTSQAGGVISFKDVRSCGTATAVSVPC
                     MFSNMGRKEFDDNRARNSEGLLDVLQRSGVSIFWKENDGGCKGVCDRVPNIEIKPKDHP
                     QFCDKNTCYDEVVLQNLDDEVAQMKGDKLVGFHLIGSHRPPYHQRYPDKPPPFVPDCPR
                     SDIENCSDEELVNTYDNTIRYTDFVIAEMITKLKKYEDKYNTALIYLSDHGESLGAMGL
                     YLHGTPYKFAPDDQTRVPMQVWMSPGFAKEKGMDLNCLQQKAADNRYSHDNLFSSVLGI
                     WDVSTAVYDKQLDIFSQCRTVQ"
     primer_bind     complement(2419..2435)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2443..2459)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2467..2497)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2512..2533)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2821..3409)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3590..4396)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga tcacaccgtg gaaacggatg aaggcacgaa cccagttgac ataagcctgt
      481 tcggttcgta aactgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg
      541 accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt
      601 tttgtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt
      661 ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta
      721 ggcatcacaa agtacagcat catgcgcatc acgctcggtg tgatgaaggt gaatttgttg
      781 ctggtgctct ttttcgcact ggtgctgaac tggcctttct ttcttcgttt ttattctgtt
      841 atcagtggtc tggaacatgt ccgggccggt ttcgttatct cggttcctct ggtgctgctt
      901 gccgcactca acgccgtctt tatccccttt accttccgct ggttgctcaa gcccttcttt
      961 tcgttgttga tcctgacagg ctccatcgtc agttacgcca tgctcaaata cggcgtcatc
     1021 ttcgatgcca gcatgatcca gaacatagtg gagaccaaca acagtgaggc gacctcctac
     1081 ctgaatgtgc cggtcgtgct ctggttcctg ctgaccggtg tgttgcccat ggtggtgctc
     1141 tggtcgctga aggtgcgcta tccggcaaac tggtacaagg ggctggccat cagggctggt
     1201 gctctggcct tctcgctgct gttcgtggga ggcgttgccg cactttacta tcaggattac
     1261 gtctcgatcg gccgcaatca ccggatcctg ggcaagcaga tagtgccggc caactatgtc
     1321 aacggcatct acaaatatgc ccgcgacgtg gtatttgcta cccccatccc ttatcaaccg
     1381 ctggggactg atgccaaagt cgtcgccaaa ggggataaac cgaccctgat gtttctggtg
     1441 gtgggggaga cagcccgcgg caagaacttc tcgatgaacg gctacgagaa agagaccaac
     1501 ccctttacca gtcaggccgg gggcgtgatc tccttcaagg acgtgcgctc ttgcggcacg
     1561 gccacagcgg tgtcggtgcc ctgcatgttc tccaacatgg ggcgcaagga gtttgatgac
     1621 aaccgggccc gcaacagcga aggcctgctc gatgtgctgc aaagaagcgg ggtctccatc
     1681 ttctggaagg agaacgacgg cggctgcaaa ggggtgtgcg atcgggtgcc caacatcgag
     1741 atcaagccaa aagatcaccc acagttctgc gacaagaaca cctgctatga cgaggttgta
     1801 ctgcaaaatc tcgacgacga ggtggcgcag atgaagggcg acaagctggt cggtttccat
     1861 ctgatcggca gccaccgccc gccctaccac caacgctatc cggacaaacc acccccgttc
     1921 gtaccggact gcccgcgcag cgacatcgag aactgcagcg atgaagagct ggtcaacacc
     1981 tatgacaaca ccatccgcta caccgatttt gtcatagcag agatgattac caagctgaaa
     2041 aagtatgaag ataagtacaa cacggcgttg atctacctct ctgatcacgg cgagtcgctg
     2101 ggtgcgatgg ggctctatct gcatggcacg ccctacaagt ttgcccctga cgaccagacc
     2161 cgggtaccga tgcaggtctg gatgtcgccg ggctttgcca aagagaaggg gatggatctg
     2221 aactgcctgc agcaaaaagc ggcagacaat cgctactccc atgacaacct cttctcctct
     2281 gtgctcggga tctgggatgt cagcacggcg gtgtacgaca agcagctcga tattttcagc
     2341 cagtgccgca ccgtgcagta aatcggatcc cgggcccgtc gactgcagag gcctgcatgc
     2401 aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     2461 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     2521 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     2581 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     2641 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     2701 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     2761 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     2821 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     2881 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     2941 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     3001 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     3061 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     3121 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     3181 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     3241 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     3301 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     3361 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     3421 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     3481 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     3541 atcaagccca atctgaataa tgttacaacc aattaaccaa ttctgattag aaaaactcat
     3601 cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa
     3661 aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat
     3721 cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct
     3781 cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga
     3841 atggcaaaag tttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt
     3901 catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac
     3961 gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca
     4021 ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct
     4081 ggaatgctgt ttttccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga
     4141 taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct
     4201 catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat
     4261 cgggcttccc atacaagcga tagattgtcg cacctgattg cccgacatta tcgcgagccc
     4321 atttataccc atataaatca gcatccatgt tggaatttaa tcgcggcctc gacgtttccc
     4381 gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg
     4441 ttcatgatga tatattttta tcttgtgcaa tgtaacatca gagattttga gacacgggcc
     4501 agagctgca
//
