LOCUS       62056_22175        4533 bp DNA     circular SYN 13-JUN-2020
DEFINITION  Expression vector pUC57-Kan-mcr-5 DNA, complete seuence.
ACCESSION   LC548749
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Suzuki M.
  TITLE     Expression vector pUC57-Kan-mcr-5
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4533)
  AUTHORS   Suzuki M.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) Contact:Masato Suzuki National Institute of 
            Infectious Diseases, AMR Research Center; 4-2-1 Aobacho, 
            Higashimurayama, Tokyo 189-0002, Japan
REFERENCE   3  (bases 1 to 4533)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-MAY-2020) Contact:Masato Suzuki National Institute of Infectious
            Diseases, AMR Research Center; 4-2-1 Aobacho, Higashimurayama, Tokyo
            189-0002, Japan"
FEATURES             Location/Qualifiers
     source          1..4533
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        466..494
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     gene            742..2385
                     /gene="mcr-5"
                     /label=mcr-5
     CDS             742..2385
                     /codon_start=1
                     /transl_table=11
                     /gene="mcr-5"
                     /product="lipid A phosphoethanolamine transferase MCR-5"
                     /label=mcr-5
                     /protein_id="BCG50369.1"
                     /translation="MRLSAFITFLKMRPQVRTEFLTLFISLVFTLLCNGVFWNALLAGR
                     DSLTSGTWLMLLCTGLLITGLQWLLLLLVATRWSVKPLLILLAVMTPAAVYFMRNYGVY
                     LDKAMLRNLMETDVREASELLQWRMLPYLLVAAVSVWWIARVRVLRTGWKQAVMMRSAC
                     LAGALAMISMGLWPVMDVLIPTLRENKPLRYLITPANYVISGIRVLTEQASSSADEARE
                     VVAADAHRGPQEQGRRPRALVLVVGETVRAANWGLSGYERQTTPELAARDVINFSDVTS
                     CGTDTATSLPCMFSLNGRRDYDERQIRRRESVLHVLNRSDVNILWRDNQSGCKGVCDGL
                     PFENLSSAGHPTLCHGERCLDEILLEGLAEKITTSRSDMLIVLHMLGNHGPAYFQRYPA
                     SYRRWSPTCDTTDLASCSHEALVNTYDNAVLYTDHVLARTIDLLSGIRSHDTALLYVSD
                     HGESLGEKGLYLHGIPYVIAPDEQIKVPMIWWQSSQVYADQACMQTHASRAPVSHDHLF
                     HTLLGMFDVKTAAYTPELDLLATCRKGQPQ"
     primer_bind     complement(2443..2459)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2467..2483)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2491..2521)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2536..2557)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2845..3433)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3614..4420)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga tcacaccgtg gaaacggatg aaggcacgaa cccagttgac ataagcctgt
      481 tcggttcgta aactgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg
      541 accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt
      601 tttgtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt
      661 ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta
      721 ggcatcacaa agtacagcat catgcggttg tctgcattta tcactttctt gaaaatgcgc
      781 ccgcaagtgc gcactgaatt tttgactctg ttcatcagcc ttgtgttcac cctgctgtgc
      841 aatggcgtgt tttggaatgc ccttcttgct ggacgcgact ccctaacttc tggaacatgg
      901 ctaatgctcc tttgcactgg gttgctgatc accgggctgc aatggttgtt gctccttctg
      961 gtggccacgc gctggagtgt caagccacta ctgattctgc ttgctgtcat gacgcccgcc
     1021 gccgtttatt tcatgcgcaa ctacggggtt tatctcgaca aggccatgct gcggaatctg
     1081 atggagacgg acgtcaggga agccagtgag ctgttgcaat ggagaatgct gccctacttg
     1141 ttggttgcag ccgtatccgt gtggtggatt gcgagagtca gggttttacg aacgggctgg
     1201 aaacaagcgg taatgatgcg cagcgcttgt ctggctggcg ctctcgccat gatttccatg
     1261 ggtctgtggc cagtcatgga tgtgctgata cccacgcttc gtgaaaacaa gccgcttcgc
     1321 tatttgatca ctcctgcaaa ctacgtcatc tcgggcattc gggttttgac tgaacaggcg
     1381 tcatcgtcag cagacgaagc aagggaagtc gttgcagccg atgcgcatcg agggcctcaa
     1441 gaacaaggcc gccgtcctcg tgctctcgta ctggttgtcg gggaaaccgt cagggcggct
     1501 aattgggggt tgagcggcta tgaacgacaa accacccctg agttggccgc acgcgacgtg
     1561 atcaattttt ccgatgtcac cagttgcggg acggatacgg ctacatccct tccctgcatg
     1621 ttttccctca atggtcggcg cgactacgac gaacgccaga ttcgtcggcg cgagtccgtg
     1681 ctgcacgttt taaaccgtag tgacgtcaac attctctggc gcgataacca gtcgggctgt
     1741 aaaggcgtct gtgatggact gccctttgaa aacctgtctt cggcaggcca tcccacactg
     1801 tgccatggcg agcgctgcct ggatgaaatt ctgctcgaag ggttggccga gaagataaca
     1861 acaagccgca gcgatatgct gatcgttctg catatgctgg gcaatcacgg cccagcgtat
     1921 ttccagcgct atcccgcaag ctaccgacgc tggtcgccaa cctgcgacac caccgatctg
     1981 gccagctgtt cgcatgaagc cttggtgaac acctacgaca acgccgtgct ttacaccgat
     2041 catgtgcttg cccgtaccat tgacctgctg tccggcatcc gctcacacga cacggcgctg
     2101 ctgtacgttt ccgatcatgg ggaatcgctc ggcgagaaag gcctgtatct ccatggcata
     2161 ccttacgtca tcgcgccgga tgagcagatc aaggtgccga tgatctggtg gcagtcgagt
     2221 caggtttatg ccgaccaagc ctgtatgcaa actcatgcct ctcgggcacc ggtaagtcac
     2281 gatcacctgt ttcacacctt gctcgggatg ttcgacgtga aaaccgctgc ctacacgcca
     2341 gagttggacc ttctggcaac atgcagaaaa ggacaaccac aatgaatcgg atcccgggcc
     2401 cgtcgactgc agaggcctgc atgcaagctt ggcgtaatca tggtcatagc tgtttcctgt
     2461 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     2521 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     2581 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     2641 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     2701 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     2761 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     2821 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     2881 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     2941 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     3001 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     3061 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     3121 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     3181 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     3241 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     3301 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     3361 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     3421 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     3481 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     3541 tttaaattaa aaatgaagtt ttaaatcaag cccaatctga ataatgttac aaccaattaa
     3601 ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag
     3661 gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga
     3721 ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact cgtccaacat
     3781 caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat
     3841 gagtgacgac tgaatccggt gagaatggca aaagtttatg catttctttc cagacttgtt
     3901 caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca
     3961 ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct gttaaaagga caattacaaa
     4021 caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata ttttcacctg
     4081 aatcaggata ttcttctaat acctggaatg ctgtttttcc ggggatcgca gtggtgagta
     4141 accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg
     4201 tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat
     4261 gtttcagaaa caactctggc gcatcgggct tcccatacaa gcgatagatt gtcgcacctg
     4321 attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat
     4381 ttaatcgcgg cctcgacgtt tcccgttgaa tatggctcat aacacccctt gtattactgt
     4441 ttatgtaagc agacagtttt attgttcatg atgatatatt tttatcttgt gcaatgtaac
     4501 atcagagatt ttgagacacg ggccagagct gca
//
