LOCUS       62056_22165        4515 bp DNA     circular SYN 13-JUN-2020
DEFINITION  Expression vector pUC57-Kan-mcr-3 DNA, complete seuence.
ACCESSION   LC548747
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4515)
  AUTHORS   Suzuki M.
  TITLE     Bacterial ARG expression libraries
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4515)
  AUTHORS   Suzuki M.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) Contact:Masato Suzuki National Institute of 
            Infectious Diseases, AMR Research Center; 4-2-1 Aobacho, 
            Higashimurayama, Tokyo 89-0002, Japan
REFERENCE   3  (bases 1 to 4515)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-MAY-2020) Contact:Masato Suzuki National Institute of Infectious
            Diseases, AMR Research Center; 4-2-1 Aobacho, Higashimurayama, Tokyo
            89-0002, Japan"
FEATURES             Location/Qualifiers
     source          1..4515
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        466..494
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     gene            742..2367
                     /gene="mcr-3"
                     /label=mcr-3
     CDS             742..2367
                     /codon_start=1
                     /transl_table=11
                     /gene="mcr-3"
                     /product="lipid A phosphoethanolamine transferase MCR-3"
                     /label=mcr-3
                     /protein_id="BCG50365.1"
                     /translation="MPSLIKIKIVPLMFFLALYFAFMLNWRGVLHFYEILYKLEDFKFG
                     FAISLPILLVAALNFVFVPFSIRYLIKPFFALLIALSAIVSYTMMKYRVLFDQNMIQNI
                     FETNQNEALAYLSLPIIVWVTIAGFIPAILLFFVEIEYEEKWFKGILTRALSMFASLIV
                     IAVIAALYYQDYVSVGRNNSNLQREIVPANFVNSTVKYVYNRYLAEPIPFTTLGDDAKR
                     DTNQSKPTLMFLVVGETARGKNFSMNGYEKDTNPFTSKSGGVISFNDVRSCGTATAVSV
                     PCMFSNMGRKEFDDNRARNSEGLLDVLQKTGISIFWKENDGGCKGVCDRVPNIEIEPKD
                     HPKFCDKNTCYDEVVLQDLDSEIAQMKGDKLVGFHLIGSHGPTYYKRYPDAHRQFTPDC
                     PRSDIENCTDEELTNTYDNTIRYTDFVIGEMIAKLKTYEDKYNTALLYVSDHGESLGAL
                     GLYLHGTPYQFAPDDQTRVPMQVWMSPGFTKEKGVDMACLQQKAADTRYSHDNIFSSVL
                     GIWDVKTSVYEKGLDIFSQCRNVQ"
     primer_bind     complement(2425..2441)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2449..2465)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2473..2503)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2518..2539)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2827..3415)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3596..4402)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga tcacaccgtg gaaacggatg aaggcacgaa cccagttgac ataagcctgt
      481 tcggttcgta aactgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg
      541 accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt
      601 tttgtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt
      661 ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta
      721 ggcatcacaa agtacagcat catgccttcc cttataaaaa taaaaattgt tccgcttatg
      781 ttctttttgg cactgtattt tgcatttatg ctgaactggc gtggagttct ccatttttac
      841 gaaatccttt acaaattaga agattttaag tttggtttcg ccatttcatt accaatattg
      901 cttgttgcag cgcttaactt tgtatttgtt ccattttcga tacggtattt aataaagcct
      961 ttttttgcac ttcttatcgc acttagtgca atcgttagtt acacaatgat gaagtataga
     1021 gtcttgtttg atcaaaacat gattcagaat atttttgaaa ccaatcaaaa tgaggcgtta
     1081 gcatatttaa gcttaccaat tatagtatgg gttactattg ctggttttat ccctgccatt
     1141 ttacttttct ttgttgaaat tgaatatgag gaaaaatggt tcaaagggat tctaactcgt
     1201 gccctatcga tgtttgcatc acttatagtg attgcggtta ttgcagcact atactatcaa
     1261 gattatgtgt cagtggggcg caacaattca aacctccagc gtgagattgt tccagccaat
     1321 ttcgttaata gtaccgttaa atacgtttac aatcgttatc ttgctgaacc aatcccattt
     1381 acaactttag gtgatgatgc aaaacgggat actaatcaaa gtaagcccac gttgatgttt
     1441 ctggtcgttg gtgaaaccgc tcgtggtaaa aatttctcga tgaatggcta tgagaaagac
     1501 accaatccat ttaccagtaa atctggtggc gtgatctcct ttaatgatgt tcgttcgtgt
     1561 gggactgcaa ccgctgtatc cgtcccctgc atgttctcca atatggggag aaaggagttt
     1621 gatgataatc gcgctcgcaa tagcgagggc ctgctagatg tgttgcaaaa aacggggatc
     1681 tccatttttt ggaaggagaa cgatggaggc tgcaaaggcg tctgcgaccg agtacctaac
     1741 atcgaaatcg aaccaaagga tcaccctaag ttctgcgata aaaacacatg ctatgacgag
     1801 gttgtccttc aagacctcga tagtgaaatt gctcaaatga aaggggataa gctggttggc
     1861 ttccacctga taggtagcca tggcccaacc tactacaagc gctaccctga tgctcatcgt
     1921 cagttcaccc ctgactgtcc acgcagtgat attgaaaact gcacagatga agagctcacc
     1981 aacacctatg acaacaccat ccgctacacc gatttcgtga ttggagagat gattgccaag
     2041 ttgaaaacct acgaagataa gtacaacacc gcgttgctct acgtctccga tcatggtgaa
     2101 tcactgggag cattagggct ttacctacac ggtacaccgt accagtttgc accggatgat
     2161 cagacccgtg ttcctatgca ggtgtggatg tcacctggat ttaccaaaga gaaaggcgtt
     2221 gatatggcgt gtttgcagca gaaagccgct gatactcgtt actcacacga taatattttc
     2281 tcatctgtat tgggtatctg ggacgtcaaa acatcagttt acgaaaaggg tctagatatt
     2341 ttcagtcaat gtcgtaatgt tcaataaatc ggatcccggg cccgtcgact gcagaggcct
     2401 gcatgcaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     2461 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     2521 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     2581 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     2641 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     2701 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     2761 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     2821 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     2881 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     2941 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     3001 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     3061 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     3121 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     3181 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     3241 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     3301 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     3361 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     3421 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     3481 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     3541 ttttaaatca agcccaatct gaataatgtt acaaccaatt aaccaattct gattagaaaa
     3601 actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt
     3661 tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg
     3721 caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt
     3781 tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg
     3841 gtgagaatgg caaaagttta tgcatttctt tccagacttg ttcaacaggc cagccattac
     3901 gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag
     3961 cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc
     4021 ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta
     4081 atacctggaa tgctgttttt ccggggatcg cagtggtgag taaccatgca tcatcaggag
     4141 tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga
     4201 ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg
     4261 gcgcatcggg cttcccatac aagcgataga ttgtcgcacc tgattgcccg acattatcgc
     4321 gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc ggcctcgacg
     4381 tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa gcagacagtt
     4441 ttattgttca tgatgatata tttttatctt gtgcaatgta acatcagaga ttttgagaca
     4501 cgggccagag ctgca
//
