LOCUS       V016326                 4515 bp    DNA     circular SYN 18-SEP-2019
DEFINITION  Exported.
ACCESSION   V016326
VERSION     V016326
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4515)
  AUTHORS   Suzuki M.
  TITLE     Bacterial ARG expression libraries
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4515)
  AUTHORS   Suzuki M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2019) Contact:Masato Suzuki National Institute of
            Infectious Diseases, AMR Research Center; 4-2-1 Aobacho,
            Higashimurayama, Tokyo 189-0002, Japan
REFERENCE   3  (bases 1 to 4515)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (04-SEP-2019) Contact:Masato Suzuki National Institute of Infectious
            Diseases, AMR Research Center; 4-2-1 Aobacho, Higashimurayama, Tokyo
            189-0002, Japan"
FEATURES             Location/Qualifiers
     source          1..4515
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        466..494
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     CDS             742..2364
                     /gene="mcr1"
                     /label="Probable phosphatidylethanolamine transferase
                     Mcr-1"
                     /note="Probable phosphatidylethanolamine transferase Mcr-1
                     from Escherichia coli. Accession#: A0A0R6L508"
     primer_bind     complement(2425..2441)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2449..2465)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2473..2503)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2518..2539)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2827..3415)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3596..4402)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga tcacaccgtg gaaacggatg aaggcacgaa cccagttgac ataagcctgt
      481 tcggttcgta aactgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg
      541 accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt
      601 tttgtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt
      661 ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta
      721 ggcatcacaa agtacagcat catgatgcag catacttctg tgtggtaccg acgctcggtc
      781 agtccgtttg ttcttgtggc gagtgttgcc gttttcttga ccgcgaccgc caatcttacc
      841 ttttttgata aaatcagcca aacctatccc atcgcggaca atctcggctt tgtgctgacg
      901 atcgctgtcg tgctctttgg cgcgatgcta ctgatcacca cgctgttatc atcgtatcgc
      961 tatgtgctaa agcctgtgtt gattttgcta ttaatcatgg gcgcggtgac cagttatttt
     1021 actgacactt atggcacggt ctatgatacg accatgctcc aaaatgccct acagaccgac
     1081 caagccgaga ccaaggatct attaaacgca gcgtttatca tgcgtatcat tggtttgggt
     1141 gtgctaccaa gtttgcttgt ggcttttgtt aaggtggatt atccgacttg gggcaagggt
     1201 ttgatgcgcc gattgggctt gatcgtggca agtcttgcgc tgattttact gcctgtggtg
     1261 gcgttcagca gtcattatgc cagtttcttt cgcgtgcata agccgctgcg tagctatgtc
     1321 aatccgatca tgccaatcta ctcggtgggt aagcttgcca gtattgagta taaaaaagcc
     1381 agtgcgccaa aagataccat ttatcacgcc aaagacgcgg tacaagcaac caagcctgat
     1441 atgcgtaagc cacgcctagt ggtgttcgtc gtcggtgaga cggcacgcgc cgatcatgtc
     1501 agcttcaatg gctatgagcg cgatactttc ccacagcttg ccaagatcga tggcgtgacc
     1561 aattttagca atgtcacatc gtgcggcaca tcgacggcgt attctgtgcc gtgtatgttc
     1621 agctatctgg gcgcggatga gtatgatgtc gataccgcca aataccaaga aaatgtgctg
     1681 gatacgctgg atcgcttggg cgtaagtatc ttgtggcgtg ataataattc ggactcaaaa
     1741 ggcgtgatgg ataagctgcc aaaagcgcaa tttgccgatt ataaatccgc gaccaacaac
     1801 gccatctgca acaccaatcc ttataacgaa tgccgcgatg tcggtatgct cgttggctta
     1861 gatgactttg tcgctgccaa taacggcaaa gatatgctga tcatgctgca ccaaatgggc
     1921 aatcacgggc ctgcgtattt taagcgatat gatgaaaagt ttgccaaatt cacgccagtg
     1981 tgtgaaggta atgagcttgc caagtgcgaa catcagtcct tgatcaatgc ttatgacaat
     2041 gccttgcttg ccaccgatga tttcatcgct caaagtatcc agtggctgca gacgcacagc
     2101 aatgcctatg atgtctcaat gctgtatgtc agcgatcatg gcgaaagtct gggtgagaac
     2161 ggtgtctatc tacatggtat gccaaatgcc tttgcaccaa aagaacagcg cagtgtgcct
     2221 gcatttttct ggacggataa gcaaactggc atcacgccaa tggcaaccga taccgtcctg
     2281 acccatgacg cgatcacgcc gacattatta aagctgtttg atgtcaccgc ggacaaagtc
     2341 aaagaccgca ccgcattcat ccgctgaatc ggatcccggg cccgtcgact gcagaggcct
     2401 gcatgcaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     2461 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     2521 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     2581 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     2641 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     2701 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     2761 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     2821 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     2881 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     2941 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     3001 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     3061 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     3121 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     3181 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     3241 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     3301 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     3361 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     3421 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     3481 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     3541 ttttaaatca agcccaatct gaataatgtt acaaccaatt aaccaattct gattagaaaa
     3601 actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt
     3661 tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg
     3721 caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt
     3781 tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg
     3841 gtgagaatgg caaaagttta tgcatttctt tccagacttg ttcaacaggc cagccattac
     3901 gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag
     3961 cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc
     4021 ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta
     4081 atacctggaa tgctgttttt ccggggatcg cagtggtgag taaccatgca tcatcaggag
     4141 tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga
     4201 ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg
     4261 gcgcatcggg cttcccatac aagcgataga ttgtcgcacc tgattgcccg acattatcgc
     4321 gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc ggcctcgacg
     4381 tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa gcagacagtt
     4441 ttattgttca tgatgatata tttttatctt gtgcaatgta acatcagaga ttttgagaca
     4501 cgggccagag ctgca
//
