LOCUS       62056_22145        3690 bp DNA     circular SYN 13-JUN-2020
DEFINITION  Expression vector pUC57-Kan-blaVIM-2 DNA, complete seuence.
ACCESSION   LC548757
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Suzuki M.
  TITLE     Bacterial ARG expression libraries
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Suzuki M.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) Contact:Masato Suzuki National Institute of 
            Infectious Diseases, AMR Research Center; 4-2-1 Aobacho, 
            Higashimurayama, Tokyo 189-0002, Japan
REFERENCE   3  (bases 1 to 3690)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-MAY-2020) Contact:Masato Suzuki National Institute of Infectious
            Diseases, AMR Research Center; 4-2-1 Aobacho, Higashimurayama, Tokyo
            189-0002, Japan"
FEATURES             Location/Qualifiers
     source          1..3690
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        466..494
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     gene            742..1542
                     /gene="blaVIM-2"
                     /label=blaVIM-2
     CDS             742..1542
                     /codon_start=1
                     /transl_table=11
                     /gene="blaVIM-2"
                     /product="class B beta-lactamase VIM-2"
                     /label=blaVIM-2
                     /protein_id="BCG50385.1"
                     /translation="MFKLLSKLLVYLTASIMAIASPLAFSVDSSGEYPTVSEIPVGEVR
                     LYQIADGVWSHIATQSFDGAVYPSNGLIVRDGDELLLIDTAWGAKNTAALLAEIEKQIG
                     LPVTRAVSTHFHDDRVGGVDVLRAAGVATYASPSTRRLAEVEGNEIPTHSLEGLSSSGD
                     AVRFGPVELFYPGAAHSTDNLVVYVPSASVLYGGCAIYELSRTSAGNVADADLAEWPTS
                     IERIQQHYPEAQFVIPGHGLPGGLDLLKHTTNVVKAHTNRSVVE"
     primer_bind     complement(1600..1616)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1624..1640)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1648..1678)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1693..1714)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2002..2590)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2771..3577)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga tcacaccgtg gaaacggatg aaggcacgaa cccagttgac ataagcctgt
      481 tcggttcgta aactgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg
      541 accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt
      601 tttgtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt
      661 ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta
      721 ggcatcacaa agtacagcat catgttcaaa cttttgagta agttattggt ctatttgacc
      781 gcgtctatca tggctattgc gagtccgctc gctttttccg tagattctag cggtgagtat
      841 ccgacagtca gcgaaattcc ggtcggggag gtccggcttt accagattgc cgatggtgtt
      901 tggtcgcata tcgcaacgca gtcgtttgat ggcgcagtct acccgtccaa tggtctcatt
      961 gtccgtgatg gtgatgagtt gcttttgatt gatacagcgt ggggtgcgaa aaacacagcg
     1021 gcacttctcg cggagattga gaagcaaatt ggacttcctg taacgcgtgc agtctccacg
     1081 cactttcatg acgaccgcgt cggcggcgtt gatgtccttc gggcggctgg ggtggcaacg
     1141 tacgcatcac cgtcgacacg ccggctagcc gaggtagagg ggaacgagat tcccacgcac
     1201 tctctagaag gactctcatc gagcggggac gcagtgcgct tcggtccagt agaactcttc
     1261 tatcctggtg ctgcgcattc gaccgacaac ttagttgtgt acgtcccgtc tgcgagtgtg
     1321 ctctatggtg gttgtgcgat ttatgagttg tcacgcacgt ctgcggggaa cgtggccgat
     1381 gccgatctgg ctgaatggcc cacctccatt gagcggattc aacaacacta cccggaagca
     1441 cagttcgtca ttccggggca cggcctgccg ggcggtctag acttgctcaa gcacacaacg
     1501 aatgttgtaa aagcgcacac aaatcgctca gtcgttgagt agatcggatc ccgggcccgt
     1561 cgactgcaga ggcctgcatg caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     1621 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     1681 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     1741 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     1801 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     1861 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     1921 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     1981 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     2041 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     2101 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     2161 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     2221 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     2281 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     2341 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     2401 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     2461 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     2521 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     2581 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     2641 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     2701 aaattaaaaa tgaagtttta aatcaagccc aatctgaata atgttacaac caattaacca
     2761 attctgatta gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat
     2821 tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc
     2881 agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa
     2941 tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag
     3001 tgacgactga atccggtgag aatggcaaaa gtttatgcat ttctttccag acttgttcaa
     3061 caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc
     3121 gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag
     3181 gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat
     3241 caggatattc ttctaatacc tggaatgctg tttttccggg gatcgcagtg gtgagtaacc
     3301 atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca
     3361 gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt
     3421 tcagaaacaa ctctggcgca tcgggcttcc catacaagcg atagattgtc gcacctgatt
     3481 gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta
     3541 atcgcggcct cgacgtttcc cgttgaatat ggctcataac accccttgta ttactgttta
     3601 tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca atgtaacatc
     3661 agagattttg agacacgggc cagagctgca
//
