LOCUS       62056_22140        3687 bp DNA     circular SYN 13-JUN-2020
DEFINITION  Expression vector pUC57-Kan-blaOXA-48 DNA, complete seuence.
ACCESSION   LC548759
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Suzuki M.
  TITLE     Bacterial ARG expression libraries
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Suzuki M.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) Contact:Masato Suzuki AMR Research Center, 
            National Institute of Infectious Diseases; 4-2-1 Aobacho, 
            Higashimurayama, Tokyo 189-0002, Japan
REFERENCE   3  (bases 1 to 3687)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-MAY-2020) Contact:Masato Suzuki AMR Research Center, National 
            Institute of Infectious Diseases; 4-2-1 Aobacho, Higashimurayama, 
            Tokyo 189-0002, Japan"
FEATURES             Location/Qualifiers
     source          1..3687
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        466..494
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     gene            742..1539
                     /gene="blaOXA-48"
                     /label=blaOXA-48
     CDS             742..1539
                     /codon_start=1
                     /transl_table=11
                     /gene="blaOXA-48"
                     /product="class D beta-lactamase OXA-48"
                     /label=blaOXA-48
                     /protein_id="BCG50389.1"
                     /translation="MRVLALSAVFLVASIIGMPAVAKEWQENKSWNAHFTEHKSQGVVV
                     LWNENKQQGFTNNLKRANQAFLPASTFKIPNSLIALDLGVVKDEHQVFKWDGQTRDIAT
                     WNRDHNLITAMKYSVVPVYQEFARQIGEARMSKMLHAFDYGNEDISGNVDSFWLDGGIR
                     ISATEQISFLRKLYHNKLHVSERSQRIVKQAMLTEANGDYIIRAKTGYSTRIEPKIGWW
                     VGWVELDDNVWFFAMNMDMPTSDGLGLRQAITKEVLKQEKIIP"
     primer_bind     complement(1597..1613)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1621..1637)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1645..1675)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1690..1711)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1999..2587)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2768..3574)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga tcacaccgtg gaaacggatg aaggcacgaa cccagttgac ataagcctgt
      481 tcggttcgta aactgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg
      541 accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt
      601 tttgtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt
      661 ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta
      721 ggcatcacaa agtacagcat catgcgtgta ttagccttat cggctgtgtt tttggtggca
      781 tcgattatcg gaatgcctgc ggtagcaaag gaatggcaag aaaacaaaag ttggaatgct
      841 cactttactg aacataaatc acagggcgta gttgtgctct ggaatgagaa taagcagcaa
      901 ggatttacca ataatcttaa acgggcgaac caagcatttt tacccgcatc tacctttaaa
      961 attcccaata gcttgatcgc cctcgatttg ggcgtggtta aggatgaaca ccaagtcttt
     1021 aagtgggatg gacagacgcg cgatatcgcc acttggaatc gcgatcataa tctaatcacc
     1081 gcgatgaaat attcagttgt gcctgtttat caagaatttg cccgccaaat tggcgaggca
     1141 cgtatgagca agatgctaca tgctttcgat tatggtaatg aggacatttc gggcaatgta
     1201 gacagtttct ggctcgacgg tggtattcga atttcggcca cggagcaaat cagcttttta
     1261 agaaagctgt atcacaataa gttacacgta tcggagcgca gccagcgtat tgtcaaacaa
     1321 gccatgctga ccgaagccaa tggtgactat attattcggg ctaaaactgg atactcgact
     1381 agaatcgaac ctaagattgg ctggtgggtc ggttgggttg aacttgatga taatgtgtgg
     1441 ttttttgcga tgaatatgga tatgcccaca tcggatggtt tagggctgcg ccaagccatc
     1501 acaaaagaag tgctcaaaca ggaaaaaatt attccctaga tcggatcccg ggcccgtcga
     1561 ctgcagaggc ctgcatgcaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     1621 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     1681 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     1741 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     1801 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     1861 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     1921 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     1981 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     2041 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2101 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     2161 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     2221 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     2281 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     2341 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     2401 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     2461 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     2521 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     2581 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     2641 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     2701 ttaaaaatga agttttaaat caagcccaat ctgaataatg ttacaaccaa ttaaccaatt
     2761 ctgattagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat
     2821 caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt
     2881 tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac
     2941 aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga
     3001 cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc tttccagact tgttcaacag
     3061 gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg
     3121 attgcgcctg agcgagacga aatacgcgat cgctgttaaa aggacaatta caaacaggaa
     3181 tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag
     3241 gatattcttc taatacctgg aatgctgttt ttccggggat cgcagtggtg agtaaccatg
     3301 catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc
     3361 agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca
     3421 gaaacaactc tggcgcatcg ggcttcccat acaagcgata gattgtcgca cctgattgcc
     3481 cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc
     3541 gcggcctcga cgtttcccgt tgaatatggc tcataacacc ccttgtatta ctgtttatgt
     3601 aagcagacag ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga
     3661 gattttgaga cacgggccag agctgca
//
