LOCUS       V016222                 3702 bp    DNA     circular SYN 13-JUN-2020
DEFINITION  Exported.
ACCESSION   V016222
VERSION     V016222
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Suzuki M.
  TITLE     Bacterial ARG expression libraries
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Suzuki M.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) Contact:Masato Suzuki National Institute of
            Infectious Diseases, AMR Research Center; 4-2-1 Aobacho,
            Higashimurayama, Tokyo 189-0002, Japan
REFERENCE   3  (bases 1 to 3702)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (19-MAY-2020) Contact:Masato Suzuki National Institute of Infectious
            Diseases, AMR Research Center; 4-2-1 Aobacho, Higashimurayama, Tokyo
            189-0002, Japan"
FEATURES             Location/Qualifiers
     source          1..3702
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        466..494
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     CDS             742..1551
                     /gene="blaNDM-1"
                     /label="Metallo-beta-lactamase type 2"
                     /note="Metallo-beta-lactamase type 2 from Klebsiella
                     pneumoniae. Accession#: C7C422"
     primer_bind     complement(1612..1628)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1636..1652)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1660..1690)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1705..1726)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2014..2602)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2783..3589)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga tcacaccgtg gaaacggatg aaggcacgaa cccagttgac ataagcctgt
      481 tcggttcgta aactgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg
      541 accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt
      601 tttgtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt
      661 ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta
      721 ggcatcacaa agtacagcat catggaattg cccaatatta tgcacccggt cgcgaagctg
      781 agcaccgcat tagccgctgc attgatgctg agcgggtgca tgcccggtga aatccgcccg
      841 acgattggcc agcaaatgga aactggcgac caacggtttg gcgatctggt tttccgccag
      901 ctcgcaccga atgtctggca gcacacttcc tatctcgaca tgccgggttt cggggcagtc
      961 gcttccaacg gtttgatcgt cagggatggc ggccgcgtgc tggtggtcga taccgcctgg
     1021 accgatgacc agaccgccca gatcctcaac tggatcaagc aggagatcaa cctgccggtc
     1081 gcgctggcgg tggtgactca cgcgcatcag gacaagatgg gcggtatgga cgcgctgcat
     1141 gcggcgggga ttgcgactta tgccaatgcg ttgtcgaacc agcttgcccc gcaagagggg
     1201 atggttgcgg cgcaacacag cctgactttc gccgccaatg gctgggtcga accagcaacc
     1261 gcgcccaact ttggcccgct caaggtattt taccccggcc ccggccacac cagtgacaat
     1321 atcaccgttg ggatcgacgg caccgacatc gcttttggtg gctgcctgat caaggacagc
     1381 aaggccaagt cgctcggcaa tctcggtgat gccgacactg agcactacgc cgcgtcagcg
     1441 cgcgcgtttg gtgcggcgtt ccccaaggcc agcatgatcg tgatgagcca ttccgccccc
     1501 gatagccgcg ccgcaatcac tcatacggcc cgcatggccg acaagctgcg ctgaatcgga
     1561 tcccgggccc gtcgactgca gaggcctgca tgcaagcttg gcgtaatcat ggtcatagct
     1621 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     1681 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     1741 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     1801 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     1861 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     1921 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     1981 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     2041 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     2101 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     2161 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     2221 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     2281 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     2341 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     2401 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     2461 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     2521 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     2581 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     2641 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     2701 ctagatcctt ttaaattaaa aatgaagttt taaatcaagc ccaatctgaa taatgttaca
     2761 accaattaac caattctgat tagaaaaact catcgagcat caaatgaaac tgcaatttat
     2821 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
     2881 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
     2941 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
     3001 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc
     3061 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
     3121 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac
     3181 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
     3241 tttcacctga atcaggatat tcttctaata cctggaatgc tgtttttccg gggatcgcag
     3301 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
     3361 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
     3421 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaag cgatagattg
     3481 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
     3541 tgttggaatt taatcgcggc ctcgacgttt cccgttgaat atggctcata acaccccttg
     3601 tattactgtt tatgtaagca gacagtttta ttgttcatga tgatatattt ttatcttgtg
     3661 caatgtaaca tcagagattt tgagacacgg gccagagctg ca
//
