LOCUS       V015609                 3771 bp    DNA     circular SYN 13-JUN-2020
DEFINITION  Exported.
ACCESSION   V015609
VERSION     V015609
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Suzuki M.
  TITLE     Bacterial ARG expression libraries
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Suzuki M.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) Contact:Masato Suzuki National Institute of
            Infectious Diseases, AMR Research Center; 4-2-1 Aobacho,
            Higashimurayama, Tokyo 189-0002, Japan
REFERENCE   3  (bases 1 to 3771)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (19-MAY-2020) Contact:Masato Suzuki National Institute of Infectious
            Diseases, AMR Research Center; 4-2-1 Aobacho, Higashimurayama, Tokyo
            189-0002, Japan"
FEATURES             Location/Qualifiers
     source          1..3771
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        466..494
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     CDS             742..1620
                     /gene="bla"
                     /label="Carbapenem-hydrolyzing beta-lactamase KPC"
                     /note="Carbapenem-hydrolyzing beta-lactamase KPC from
                     Klebsiella oxytoca. Accession#: Q848S6"
     primer_bind     complement(1681..1697)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1705..1721)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1729..1759)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1774..1795)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2083..2671)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2852..3658)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga tcacaccgtg gaaacggatg aaggcacgaa cccagttgac ataagcctgt
      481 tcggttcgta aactgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg
      541 accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt
      601 tttgtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt
      661 ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta
      721 ggcatcacaa agtacagcat catgtcactg tatcgccgtc tagttctgct gtcttgtctc
      781 tcatggccgc tggctggctt ttctgccacc gcgctgacca acctcgtcgc ggaaccattc
      841 gctaaactcg aacaggactt tggcggctcc atcggtgtgt acgcgatgga taccggctca
      901 ggcgcaactg taagttaccg cgctgaggag cgcttcccac tgtgcagctc attcaagggc
      961 tttcttgctg ccgctgtgct ggctcgcagc cagcagcagg ccggcttgct ggacacaccc
     1021 atccgttacg gcaaaaatgc gctggttccg tggtcaccca tctcggaaaa atatctgaca
     1081 acaggcatga cggtggcgga gctgtccgcg gccgccgtgc aatacagtga taacgccgcc
     1141 gccaatttgt tgctgaagga gttgggcggc ccggccgggc tgacggcctt catgcgctct
     1201 atcggcgata ccacgttccg tctggaccgc tgggagctgg agctgaactc cgccatccca
     1261 ggcgatgcgc gcgatacctc atcgccgcgc gccgtgacgg aaagcttaca aaaactgaca
     1321 ctgggctctg cactggctgc gccgcagcgg cagcagtttg ttgattggct aaagggaaac
     1381 acgaccggca accaccgcat ccgcgcggcg gtgccggcag actgggcagt cggagacaaa
     1441 accggaacct gcggagtgta tggcacggca aatgactatg ccgtcgtctg gcccactggg
     1501 cgcgcaccta ttgtgttggc cgtctacacc cgggcgccta acaaggatga caagcacagc
     1561 gaggccgtca tcgccgctgc ggctagactc gcgctcgagg gattgggcgt caacgggcag
     1621 taaatcggat cccgggcccg tcgactgcag aggcctgcat gcaagcttgg cgtaatcatg
     1681 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     1741 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     1801 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     1861 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     1921 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     1981 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     2041 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     2101 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     2161 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     2221 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     2281 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     2341 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     2401 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     2461 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     2521 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     2581 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     2641 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     2701 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     2761 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaagcc caatctgaat
     2821 aatgttacaa ccaattaacc aattctgatt agaaaaactc atcgagcatc aaatgaaact
     2881 gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg
     2941 aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga
     3001 ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat
     3061 caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca
     3121 tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat
     3181 caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt
     3241 taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat
     3301 caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct gtttttccgg
     3361 ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg
     3421 gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg
     3481 caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaagc
     3541 gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat
     3601 cagcatccat gttggaattt aatcgcggcc tcgacgtttc ccgttgaata tggctcataa
     3661 caccccttgt attactgttt atgtaagcag acagttttat tgttcatgat gatatatttt
     3721 tatcttgtgc aatgtaacat cagagatttt gagacacggg ccagagctgc a
//
