LOCUS       V016577                 3630 bp    DNA     circular SYN 13-JUN-2020
DEFINITION  Exported.
ACCESSION   V016577
VERSION     V016577
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Suzuki M.
  TITLE     Bacterial ARG expression libraries
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Suzuki M.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) Contact:Masato Suzuki National Institute of
            Infectious Diseases, AMR Research Center; 4-2-1 Aobacho,
            Higashimurayama, Tokyo 189-0002, Japan
REFERENCE   3  (bases 1 to 3630)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (19-MAY-2020) Contact:Masato Suzuki National Institute of Infectious
            Diseases, AMR Research Center; 4-2-1 Aobacho, Higashimurayama, Tokyo
            189-0002, Japan"
FEATURES             Location/Qualifiers
     source          1..3630
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        466..494
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     CDS             742..1479
                     /note="Metallo-beta-lactamase type 2 from Serratia
                     marcescens. Accession#: P52699"
                     /label="Metallo-beta-lactamase type 2"
     primer_bind     complement(1540..1556)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1564..1580)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1588..1618)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1633..1654)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1942..2530)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2711..3517)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga tcacaccgtg gaaacggatg aaggcacgaa cccagttgac ataagcctgt
      481 tcggttcgta aactgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg
      541 accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt
      601 tttgtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt
      661 ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta
      721 ggcatcacaa agtacagcat catgagcaag ttatctgtat tctttatatt tttgttttgc
      781 agcattgcta ccgcagcaga gtctttgcca gatttaaaaa ttgaaaagct tgatgaaggc
      841 gtttatgttc atacttcgtt tgaagaagtt aacgggtggg gcgttgttcc taaacatggt
      901 ttggtggttc ttgtaaatgc tgaggcttac ctaattgaca ctccatttac ggctaaagat
      961 actgaaaagt tagtcacttg gtttgtggag cgtggctata aaataaaagg cagcatttcc
     1021 tctcattttc atagcgacag cacgggcgga atagagtggc ttaattctcg atctatcccc
     1081 acgtatgcat ctgaattaac aaatgaactg cttaaaaaag acggtaaggt tcaagccaca
     1141 aattcattta gcggagttaa ctattggcta gttaaaaata aaattgaagt tttttatcca
     1201 ggcccgggac acactccaga taacgtagtg gtttggttgc ctgaaaggaa aatattattc
     1261 ggtggttgtt ttattaaacc gtacggttta ggcaatttgg gtgacgcaaa tatagaagct
     1321 tggccaaagt ccgccaaatt attaaagtcc aaatatggta aggcaaaact ggttgttcca
     1381 agtcacagtg aagttggaga cgcatcactc ttgaaactta cattagagca ggcggttaaa
     1441 gggttaaacg aaagtaaaaa accatcaaaa ccaagcaact aaatcggatc ccgggcccgt
     1501 cgactgcaga ggcctgcatg caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     1561 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     1621 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     1681 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     1741 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     1801 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     1861 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     1921 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     1981 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     2041 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     2101 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     2161 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     2221 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     2281 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     2341 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     2401 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     2461 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     2521 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     2581 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     2641 aaattaaaaa tgaagtttta aatcaagccc aatctgaata atgttacaac caattaacca
     2701 attctgatta gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat
     2761 tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc
     2821 agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa
     2881 tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag
     2941 tgacgactga atccggtgag aatggcaaaa gtttatgcat ttctttccag acttgttcaa
     3001 caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc
     3061 gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag
     3121 gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat
     3181 caggatattc ttctaatacc tggaatgctg tttttccggg gatcgcagtg gtgagtaacc
     3241 atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca
     3301 gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt
     3361 tcagaaacaa ctctggcgca tcgggcttcc catacaagcg atagattgtc gcacctgatt
     3421 gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta
     3481 atcgcggcct cgacgtttcc cgttgaatat ggctcataac accccttgta ttactgttta
     3541 tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca atgtaacatc
     3601 agagattttg agacacgggc cagagctgca
//
